The paraoxonase L55M and Q192R gene polymorphisms and myocardial infarction in a Tunisian population

Kallel, Amani; Sediri, Yousra; Sbai, Mohamed Ali; Mourali, Mohamed Sami; Feki, Moncef; Elasmi, Monia; Taieb, Samah Haj; Sanhaji, Haïfa; Souheil, Omar; Mechmèche, Rachid; Jemaa, Riadh; Kaabachi, Naziha

doi:10.1016/j.clinbiochem.2010.08.029

Cited by 19 publications

(18 citation statements)

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“…The M/L55 polymorphism is also a significant determinant of the serum concentration of paraoxonase [10]. Several casecontrol studies have shown a positive association between PON1 R192 allele and coronary heart disease, while several other studies have shown no association [11]. This study aims to investigate the effect of cigarette smoking on PON1 activity according to PON1 L55M and PON1 Q192R gene polymorphisms and the correlation between this parameter and two biological tobacco markers: plasma thiocyanate (SCN -) and cotininuria.…”

Section: Introductionmentioning

confidence: 99%

Effect of cigarette smoking on paraoxonase 1 activity according to PON1 L55M and PON1 Q192R gene polymorphisms

Mouhamed

Ezzaher

Mechri

et al. 2011

Environ Health Prev Med

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Objective This study aims to investigate the effect of cigarette smoking on paraoxonase 1 (PON1) activity according to PON1 L55M and PON1 Q192R gene polymorphisms. Materials and methods Our sample included 300 voluntary subjects: 138 nonsmokers and 162 current smokers aged 38.47 ± 21.91 and 35.55 ± 16.03 years, respectively. PON1 activity was determined by kinetic methods. L55M and Q192R gene polymorphisms of PON1 were determined by multiplex polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP). Results We found in smokers a significant decrease of PON1 activity before and after adjustment. We noted a significant association between smoking status and lower PON1 activity [odds ratio (OR) = 3.03, confidence interval 95% = 1.5-5.9, p = 0.001]. In smokers, there was significant association between PON1 activity and PON1 L55M polymorphisms (p = 0.01). Also, the 55MM genotype presented the lowest paraoxonase activity, while the 55LL genotype showed the highest one. After adjustment for confounding variables, smokers with PON1 L55M polymorphism had the highest risk for lower PON1 activity; however, PON1 Q192R genotype might protect smokers from decrease in PON1 activity. We found significant interaction between the effect of cigarette smoking and both PON1 L55M and PON1 Q192R polymorphisms on lower PON1 activity. Conclusions Cigarette smoking was significantly associated with decrease in PON1 activity. Moreover, PON1 L55M polymorphism predisposes smokers to decreased PON1 activity in contrast to PON1 Q192R genotype.

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Section: Introductionmentioning

confidence: 99%

Effect of cigarette smoking on paraoxonase 1 activity according to PON1 L55M and PON1 Q192R gene polymorphisms

Mouhamed

Ezzaher

Mechri

et al. 2011

Environ Health Prev Med

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“…Low serum PON1 activity alleles are associated with a decreased transfer of lipids between lipoprotein particles (Ombres et al 1998). Conversely, several other studies inconsistent results regarding the link between PON1 Q192R polymorphism and CVD (Kallel et al, 2010). The RR genotype was more frequent in patients with CVD and was independently associated with the disease risk.…”

Section: Discussion:-mentioning

confidence: 78%

The Impact of Rs662 Genotyping on the Serum Activity of the Paraoxonase -1 Enzyme in Iraqi Patients With Cardiovascular Diseases.

Salih¹,

Hasan²,

Hashim³

2017

IJAR

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Background: Cardiovascular diseases (CVDs) are diseases that affect the heart or vessels. Coronary artery disease (CAD) and peripheral (PAD) are types of CVD. PON1 is a HDL-associated lipolactonase is considered to be atheroprotective by preventing LDL and cell membranes oxidation. A common polymorphism due to an amino acid substitution (Glutamine  Arginine) at codon 192 is considered to be a major determinant of variation in serum PON1 activity. Recent studies have suggested that the PON1192 polymorphism is an independent risk factor for CVD Objectives: The effect of the PON1 activity and PON1 Q192R (rs662) polymorphisms on the CVD include patients with PAD and CAD. Subject and Methods: This case control study consisted of 180 subjects were divided into 100 patients with CVD (50 patients with PAD and 50 patients with CAD), and 80 healthy controls. Serum levels for lipid profile and PON1 enzyme were measured by spectrophotometer using paraoxon as a substrate. The genetic polymorphism of PON1 was detected by TaqMan-based allele discrimination RT-PCR Results: The means ±SEM of serum PON1 activity were highly significantly decreased in PAD and CAD as compared to control group. The heterozygote (Q192R) genotype of PON1 polymorphism is abundant among all of the studied groups (68%for PAD, 56% for CAD and 65%for control, respectively) . The CAD patients carry the high risk RR genotyping (odd ratio of 2.69, with the confidence interval of (1.12-6.47; p=0.02).The PAD who carry the RR genotype have a risk ratio 1.57 at confidence interval of 0.61-4.02. Conclusion: Reduction of PON1 activity is one of the risk factor of development of CVD . Patients who carry the RR genotyping were at high risk to develop CVD. Our results indicate that controversial relationships regarding the PON1 rs 662 (Q192R) polymorphism and CVD. Serum PON1 activity is more informative than the PON1 genotype in evaluating the severity and extent of cardiovascular disease in Iraqi patients.

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“…The authors reported lower levels of PON1 activity and a lower genotype RR frequency with significant statistic differences, compared to the Jewish population, suggesting that these findings could explain, partly, the higher risk for cardiovascular disease [17]. Kallel et al [18] found a higher R allele and RR genotype frequency in subjects with myocardial infarction, reporting an association between these factors and an increase in the risk for this pathology in Tunisian population. Other investigators, in India, found an association between the R allele and the coronary disease, as well.…”

Section: Figure 1 Factors That Increase Homocysteinementioning

confidence: 97%

“…The reason of this discrepancy is yet unclear, but it is possibly caused by differences in sample size, frequency of the polymorphisms in the population, and the study population. The biological mechanisms by which these polymorphisms could be associated to the disease are not clear yet and it is needed to develop more investigation trying to elucidate if paraoxonase is involved in preventing the lipid oxidation in the circulation and if there are other mechanisms involved in the physiopathology of the coronary artery disease [18]. For instance, it has been reported that Palestinian population have a higher frequency of the coronary disease and higher mortality associated than its Jewish population counterpart, with a higher frequency of diabetes and lower HDL levels.…”

Section: Figure 1 Factors That Increase Homocysteinementioning

confidence: 99%

Evaluation of Genetic and Biochemical Factors in Patients with Acute Coronary Syndrome

et al. 2017

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Introduction: The cardiovascular diseases are the principal cause of death worldwide and a public health problem in Colombia. From the cardiac related illnesses, the most frequent is the Acute Coronary Syndrome (ACS). One of its risk factor is high plasma levels of homocysteine. The enzyme Paraoxonase 1 (PON1) hydrolyzes Homocysteine-thiolactone, producing homocysteine, avoiding damage to endothelium related to Homocysteine-thiolactone. Two genetic variants in PON1 gene have been associated with levels of homocysteine and acute coronary syndrome. Objective: to determine the relationship between the polymorphisms Q192R and L55M from gene PON1 and the levels of homocysteine, HDL, apo A-1 and the enzymatic activity of Paraoxonase I in patients who suffered acute coronary syndrome. Methods: sixteen unrelated patients with Acute Coronary Syndrome (ACS) and sixteen healthy controls were enrolled. The paraoxonase activity, levels of homocysteine, HDL and apo A-1 were assessed. The DNA polymorphism analysis was performed using Polymerase Chain Reaction (PCR) and digestion with restriction enzymes. Results: levels of homocysteine are higher in cases (p<0.0001) and the genotypes 192RR and QR are associated with higher levels of homocysteine (p=0.0234). Conclusion: polymorphisms in <em>PON1</em> gene could be related to homocysteine levels in Acute Coronary Syndrome patients.

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The paraoxonase L55M and Q192R gene polymorphisms and myocardial infarction in a Tunisian population

Cited by 19 publications

References 10 publications

Effect of cigarette smoking on paraoxonase 1 activity according to PON1 L55M and PON1 Q192R gene polymorphisms

Effect of cigarette smoking on paraoxonase 1 activity according to PON1 L55M and PON1 Q192R gene polymorphisms

The Impact of Rs662 Genotyping on the Serum Activity of the Paraoxonase -1 Enzyme in Iraqi Patients With Cardiovascular Diseases.

Evaluation of Genetic and Biochemical Factors in Patients with Acute Coronary Syndrome

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