The parameters of reproduction, sizes, and activities of hydrolases in Daphnia magna straus of successive generations affected by Roundup herbicide

Papchenkova, G. A.; Голованова, И. Л.; Ушакова, Н. В.

doi:10.1134/s1995082909030158

Cited by 22 publications

(14 citation statements)

References 7 publications

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“…This pattern may reflect that maternal effects in daphnids could minimize the lag between exposure to cyanobacteria and inducible defences, consequently improving offspring fitness (Marshall & Uller, 2007). However, several studies have also demonstrated that maternal effects could reduce offspring tolerance and enhance the lag between environmental stresses and inducible defences (Scheirs, De Bruyn & Verhagen, 2000;Papchenkova, Golovanova & Ushakova, 2009). For example, Daphnia offspring had higher sensitivity to glyphosate herbicide if their mother had been exposed to that herbicide, reflected in inhibited fecundity, lower quality of progeny and smaller body size (Papchenkova et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Maternal consumption of non‐toxic Microcystis by Daphnia magna induces tolerance to toxic Microcystis in offspring

Lyu

Guan

et al. 2015

Freshwater Biology

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1. Freshwater cyanobacterial blooms are a worldwide environmental issue. These blooms often comprise both non-toxic and toxic species and strains. Populations of some zooplankton, including Daphnia, have been shown to adapt locally to toxic Microcystis through maternal effects. However, Microcystis populations vary spatially and temporally in the absolute and relative abundances of non-toxic and toxic genotypes. 2. We examined variation in induction of tolerance to toxic cyanobacteria in offspring from two Daphnia magna clones (JS and AH) fed diets containing Scenedesmus by itself or in combination with either a non-toxic or a toxic clone of Microcystis aeruginosa. The diets containing Microcystis included relatively more cyanobacteria within each week of the 3 week experiment (week 1-10%, week 2-20%, week 3-40%). Daphnia neonates were collected from these three treatments at the end of the third week and fed a diet containing Scenedesmus and toxic Microcystis for 3 weeks before a suite of lifehistory, physiological and biochemical measurements were made on the surviving animals. 3. Neonates from mothers fed toxic and non-toxic Microcystis showed enhanced growth and reproduction compared to neonates produced from mothers fed only Scenedesmus. Our results showed that Daphnia neonates could be induced to tolerate toxic cyanobacteria when their mothers were fed diets containing non-toxic or toxic strains of cyanobacteria. Furthermore, elevated RNA-DNA ratios, superoxide dismutase activity and catalase activity in neonates fed diets containing non-toxic or toxic Microcystis clones suggested that the mechanisms behind these changes involved processes associated with metabolism and antioxidation. 4. Our study shows that non-toxic cyanobacteria can induce tolerance to toxic cyanobacteria and further elucidates the general importance of maternal effects on tolerance of cyanobacteria in herbivorous zooplankton.

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Section: Discussionmentioning

confidence: 99%

Maternal consumption of non‐toxic Microcystis by Daphnia magna induces tolerance to toxic Microcystis in offspring

Lyu

Guan

et al. 2015

Freshwater Biology

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“…25 and 50 mg/l a.i. for four generations, showed a significantly reduced fecundity but no adverse effect on the survival of mother animals (Papchenkova et al 2009). …”

Section: Discussionmentioning

confidence: 99%

Clone- and age-dependent toxicity of a glyphosate commercial formulation and its active ingredient in Daphnia magna

2012

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Low levels of glyphosate based herbicide induced significant negative effects on the aquatic invertebrate Daphnia magna. Glyphosate herbicides such as brands of Roundup, are known to be toxic to daphnids. However, published findings on acute toxicity show significant discrepancies and variation across several orders of magnitude. To test the acute effects of both glyphosate and a commercial formulation of Roundup (hereafter Roundup), we conducted a series of exposure experiments with different clones and age-classes of D. magna. The results demonstrated EC50 (48) values in the low ppm-range for Roundup as well as for the active ingredient (a.i.) isopropylamine salt of glyphosate (glyphosate IPA) alone. Roundup showed slightly lower acute toxicity than glyphosate IPA alone, i.e. EC50 values of 3.7–10.6 mg a.i./l, as compared to 1.4–7.2 mg a.i./l for glyphosate IPA. However, in chronic toxicity tests spanning the whole life-cycle, Roundup was more toxic. D. magna was exposed to sublethal nominal concentrations of 0.05, 0.15, 0.45, 1.35 and 4.05 mg a.i./l for 55 days. Significant reduction of juvenile size was observed even in the lowest test concentrations of 0.05 mg a.i./l, for both glyphosate and Roundup. At 0.45 mg a.i./l, growth, fecundity and abortion rate was affected, but only in animals exposed to Roundup. At 1.35 and 4.05 mg a.i./l of both glyphosate and Roundup, significant negative effects were seen on most tested parameters, including mortality. D. magna was adversely affected by a near 100 % abortion rate of eggs and embryonic stages at 1.35 mg a.i./l of Roundup. The results indicate that aquatic invertebrate ecology can be adversely affected by relevant ambient concentrations of this major herbicide. We conclude that glyphosate and Roundup toxicity to aquatic invertebrates have been underestimated and that current European Commission and US EPA toxicity classification of these chemicals need to be revised.

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“…In some cases, tolerance is not achieved or transgenerational effects are not clear, for example after exposure of F0 to the plastic additive and surfactant p-nonylphenol (Tanaka and Nakanishi, 2002) or the pharmaceuticals carbamazepine, diclofenac, 17␣-ethinylestradiol and metroprolol (Dietrich et al, 2010). Even a magnification of the detrimental effect has been shown, as was found with lauryl sulphate (Le Blanc, 1982), the pesticides tetradifon (Villarroel et al, 2000) and diazinon (Sánchez et al, 2000), the endocrine disruptors 17-oestradiol E2, diethylstilbestrol, bisphenol A and 4-nonylphenol (Brennan et al, 2006), uranium (Massarin et al, 2010) and the herbicide glyphosate (Papchenkova et al, 2009). It is important to remark that in this last set of studies, where a magnified effect was found in the subsequent generations, a clear effect was not found in the parental generations, highlighting the importance of multigenerational approaches in ecotoxicology to the understanding and evaluation of the impact of chemicals in the ecosystem.…”

Section: Discussionmentioning

confidence: 99%

Transgenerational effects of microcystin-LR onDaphnia magna

Ortiz-Rodríguez

Dao

Wiegand

2012

Journal of Experimental Biology

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SUMMARYAnthropogenic and climate factors increase the frequency of problematic cyanobacterial blooms in freshwater. Among other toxins, some cyanobacteria produce microcystins (MCs), which inhibit phosphatases type 1 and type 2A and provokes oxidative stress. Toxic cyanobacteria affect the growth, survival and reproduction of zooplankton, particularly those from the genus Daphnia, which have a central position in pelagic food webs. However, one possibility to ameliorate effects is to biotransform MC via glutathione S transferase (GST) to a less toxic glutathione conjugate. This process was hypothesised to underlie the ability of Daphnia to withstand MC and to explain the enhanced tolerance of the offspring from mothers exposed to toxic cyanobacteria. Thus we conducted multigenerational experiments with D. magna, exposing the parental generation to MC for 1 or 7days and determining the enzyme-mediated tolerance to MC in their offspring by assessing the acute effect of MC on biotransformation and antioxidant and metabolism enzymes, and through 21day chronic tests on toxicity and growth. Seven days of exposure of the parental generation to MC induced higher activity of GST and malate dehydrogenase in the offspring and enabled them to increment the catalase activity when challenged with MC, whereas 1day of exposure of the parental generation did not. Offspring from non-exposed and 1-day-exposed mothers suffered decreased survival when exposed to MC compared with offspring from 7-day-exposed mothers; survival was correlated with the elevated activity of GST, malate dehydrogenase and catalase, suggesting maternal transfer of activation factors. However, increased survival occurred at the expense of individual growth. These results suggest that transgenerational effects are provoked by MC in D. magna, which may explain the observed acquirement of enhanced tolerance over generations.Key words: microcystin, cyanobacterial toxin, transgenerational effect, Daphnia, zooplankton. THE JOURNAL OF EXPERIMENTAL BIOLOGY 2796 cyanobacteria on Daphnia are reflected by, for example, reduced growth, survival and reproduction (Lampert, 1981; Demott et al., 1991;Hietala et al., 1995;Nogueira et al., 2004;Trubetskova and Haney, 2006; Dao et al., 2010). However, one possible way to reduce toxic effects is to biotransform the toxin MC-LR by conjugation to the tripeptide glutathione (GSH) via the enzyme glutathione S transferase (GST) (Pflugmacher et al., 1998). MCs are conjugated to GSH through the terminal methylene of N-methyldehydroalanine (Kondo, 1992), which is the moiety that binds to the active center of protein phosphatases (MacKintosh, 1995). The resulting conjugate is less toxic (Metcalf et al., 2000) and more hydrophilic, for easier transportation or excretion (Pflugmacher et al., 2001). This MC-LR detoxification process has been described in several aquatic organisms ranging from plants to invertebrates (including Daphnia magna) to fish (Pflugmacher et al., 1998;Wiegand and Pflugmacher, 2005). Three major families of prote...

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The parameters of reproduction, sizes, and activities of hydrolases in Daphnia magna straus of successive generations affected by Roundup herbicide

Cited by 22 publications

References 7 publications

Maternal consumption of non‐toxic Microcystis by Daphnia magna induces tolerance to toxic Microcystis in offspring

Maternal consumption of non‐toxic Microcystis by Daphnia magna induces tolerance to toxic Microcystis in offspring

Clone- and age-dependent toxicity of a glyphosate commercial formulation and its active ingredient in Daphnia magna

Transgenerational effects of microcystin-LR onDaphnia magna

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