Aim/hypothesis. Diabetic neuropathy is accompanied by a range of positive (paresthaesia, dysesthaesia, pain) and negative (hypesthaesia, anesthaesia) neurological symptoms suggesting widespread alterations in axonal excitability. The nature and the mechanisms underlying these alterations in axonal excitability are not well understood. The aim of this study was to examine the extent of changes in membrane properties of an identified neuronal structure -the large myelinated sensory axons in early experimental diabetes in rats. Methods. Intra-axonal microelectrode recordings from large sensory myelinated axons from the isolated sural nerve in short-term streptozotocin-induced diabetic rats were used to study membrane properties using standard current-clamp technique.Results. In addition to decreased conduction velocity we found several differences in physiological properties of sensory axons from diabetic rats: decreased resting membrane potential, decreased single action potential amplitude associated with slower rate of rise and decrease in inward rectification associated with slight alteration in outwardly rectifying conductances indicating impaired potassium conductances. Conclusion/Interpretation. These results extend previous indirect evidence that potassium and sodium ionic conductances, most notably the inward rectifier (IR, I h ), are altered in large sensory axons of diabetic rats. . The pathogenesis of diabetic neuropathies and consequently their treatment remain elusive, in spite of a number of hypotheses advanced in the past decades. These hypotheses deal with several major areas, such as metabolic, ischaemia and oxidative stress, non-specific glycosylation, and disturbances in trophic factors [1,2,3,4]. The histopathological findings of advanced diabetic neuropathy (DN) are characterized by axonal degeneration, demyelination and atrophy that are associated with highly diverse disturbances of peripheral nerve function [2,5,6]. Different positive and negative neurological signs associated with DN suggest modification in axonal membrane excitability. Indeed, one of the earliest detectable physical signs of early DN is a decrease in conduction velocity as signsThe peripheral neuropathy associated with diabetes is one of the most common polyneuropathies, and at least 50% of diabetic patients will develop a form of