The paradigm of miRNA and siRNA influence in Oral-biome

Sinha, Adrija; Bhattacharjee, Rahul; Bhattacharya, Basudha; Nandi, Aditya; Shekhar, Ramya; Jana, Ankit; Saha, Koustav; Kumar, Lamha; Patro, Swadheena; Panda, Pritam Kumar; Kaushik, Nagendra Kumar; Suar, Mrutyunjay; Verma, Suresh K.

doi:10.1016/j.biopha.2023.114269

Cited by 12 publications

(6 citation statements)

References 187 publications

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“…To demonstrate that the dCas9-PD-Cu sensor can be used for the diagnosis of biological samples, we attempted the detection of nucleic acids derived from cultured bacteria. The direct detection of target RNA within a bulk solution of total RNA is extremely challenging due to the small portion of target RNA and the interference by nontarget RNAs . Therefore, we integrated loop-mediated amplification (LAMP), which can increase the copy number of target RNA in solution at isothermal conditions, to make diagnosis feasible at the bedside.…”

Section: Resultsmentioning

confidence: 99%

A Wireless, CRISPR-Polymer Dot Electrochemical Sensor for the Diagnosis of Bacterial Pneumonia and Multi-Drug Resistance

Im,

Robby,

Choi

et al. 2024

ACS Appl. Mater. Interfaces

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Rapid and accurate diagnosis is crucial for managing the global health threat posed by multidrug-resistant bacterial infections; however, current methods have limitations in either being time-consuming, labor-intensive, or requiring instruments with high costs. Addressing these challenges, we introduce a wireless electrochemical sensor integrating the CRISPR/Cas system with electroconductive polymer dot (PD) nanoparticles to rapidly detect bacterial pathogens from human sputum. To enhance the electroconductive properties, we synthesized copper-ion-immobilized PD (PD-Cu), followed by conjugation of the deactivated Cas9 protein (dCas9) onto PD-Cu-coated Si electrodes to generate the dCas9-PD-Cu sensor. The dCas9-PD-Cu sensor integrated with isothermal amplification can specifically detect target nucleic acids of multidrug-resistant bacteria, such as the antibiotic resistance genes kpc-2 and mecA. The dCas9-PD-Cu sensor exhibits high sensitivity, allowing for the detection of ∼54 femtograms of target nucleic acids, based on measuring the changes in resistivity of the Si electrodes through target capture by dCas9. Furthermore, a wireless sensing platform of the dCas9-PD-Cu sensor was established using a Bluetooth module and a microcontroller unit for detection using a smartphone. We demonstrate the feasibility of the platform in diagnosing multidrug-resistant bacterial pneumonia in patients' sputum samples, achieving 92% accuracy. The current study presents a versatile biosensor platform that can overcome the limitations of conventional diagnostics in the clinic.

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Section: Resultsmentioning

confidence: 99%

A Wireless, CRISPR-Polymer Dot Electrochemical Sensor for the Diagnosis of Bacterial Pneumonia and Multi-Drug Resistance

Im,

Robby,

Choi

et al. 2024

ACS Appl. Mater. Interfaces

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“…An important aspect that was discussed was the fact that the plasma levels of miR-184 decreased significantly after the surgical tumor resection, outlining the correlation between the tissue and plasma levels. Another research study showed that miR-184 levels are increased in the surgical margins of the tumor, rather than in other places, suggesting its potential to identify the residual disease [ 78 , 79 ]. Lin et al [ 80 ] conducted a study that reported a high level of miR-24 in the plasma of oral cancer patients compared to the healthy controls.…”

Section: Resultsmentioning

confidence: 99%

Circulating miRNA as a Biomarker in Oral Cancer Liquid Biopsy

et al. 2023

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Oral cancer is currently challenging the healthcare system, with a high incidence among the population and a poor survival rate. One of the main focuses related to this malignancy is the urge to implement a viable approach for improving its early diagnosis. By introducing the use of liquid biopsy and the identification of potential biomarkers, aiming for a noninvasive approach, new advancements offer promising perspectives in the diagnosis of oral cancer. The present review discusses the potential of circulating miRNAs as oral cancer biomarkers identified in body fluids such as serum, plasma, and saliva samples of oral cancer patients. Existing results reveal an important implication of different miRNA expressions involved in the initiation, development, progression, and metastasis rate of oral malignancy. Liquid biomarkers can play a crucial role in the development of the concept of personalized medicine, providing a wide range of clinical applications and future targeted therapies.

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“…The utilization of small RNA fragments, such as siRNA or miRNA, enables precise regulation of gene expression associated with pathologies. This method allows for temporarily silencing or blocking specific genes, becoming a key aspect in treating genetic diseases and cancers [ 141 ]. Among gene therapy technologies, antisense oligonucleotides (ASOs) represent another area of RNA application.…”

Section: Nucleic-acid-based Drug Delivery Systemsmentioning

confidence: 99%

Biomaterials in Drug Delivery: Advancements in Cancer and Diverse Therapies—Review

Drabczyk,

Kudłacik-Kramarczyk,

Jamroży

et al. 2024

IJMS

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Nano-sized biomaterials are innovative drug carriers with nanometric dimensions. Designed with biocompatibility in mind, they enable precise drug delivery while minimizing side effects. Controlled release of therapeutic substances enhances efficacy, opening new possibilities for treating neurological and oncological diseases. Integrated diagnostic-therapeutic nanosystems allow real-time monitoring of treatment effectiveness, which is crucial for therapy personalization. Utilizing biomaterials as nano-sized carriers in conjunction with drugs represents a promising direction that could revolutionize the field of pharmaceutical therapy. Such carriers represent groundbreaking drug delivery systems on a nanometric scale, designed with biocompatibility in mind, enabling precise drug delivery while minimizing side effects. Using biomaterials in synergy with drugs demonstrates significant potential for a revolutionary impact on pharmaceutical therapy. Conclusions drawn from the review indicate that nano-sized biomaterials constitute an innovative tool that can significantly improve therapy effectiveness and safety, especially in treating neurological and oncological diseases. These findings should guide researchers towards further studies to refine nano-sized biomaterials, assess their effectiveness under various pathological conditions, and explore diagnostic-therapeutic applications. Ultimately, these results underscore the promising nature of nano-sized biomaterials as advanced drug carriers, ushering in a new era in nanomedical therapy.

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The paradigm of miRNA and siRNA influence in Oral-biome

Cited by 12 publications

References 187 publications

A Wireless, CRISPR-Polymer Dot Electrochemical Sensor for the Diagnosis of Bacterial Pneumonia and Multi-Drug Resistance

A Wireless, CRISPR-Polymer Dot Electrochemical Sensor for the Diagnosis of Bacterial Pneumonia and Multi-Drug Resistance

Circulating miRNA as a Biomarker in Oral Cancer Liquid Biopsy

Biomaterials in Drug Delivery: Advancements in Cancer and Diverse Therapies—Review

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