The paracrine role of Sertoli cells on Leydig cell function

Lejeune, Hervé; Skalli, Mourad; Chatelain, Pierre; Avallet, O.; Saez, J.M.

doi:10.1007/bf00130513

Cited by 16 publications

(11 citation statements)

References 60 publications

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“…Kisspeptin may enhance steroidogenesis indirectly via Sertoli cells by thus far unknown paracrine communication. This idea is supported by previous studies, where Sertoli cell produced factors that influenced testosterone secretion by Leydig cells or affected the Leydig cell response to gonadotropins . The verification of this concept needs further studies to evaluate in detail the response of Sertoli cells and the factors released after kisspeptin stimulation.…”

Section: Discussionsupporting

confidence: 59%

Immunocytochemical localization of kisspeptin and kisspeptin receptor in the primate testis

Irfan

Ehmcke

Shahab

et al. 2016

J of Medical Primatology

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Section: Discussionsupporting

confidence: 59%

Immunocytochemical localization of kisspeptin and kisspeptin receptor in the primate testis

Irfan

Ehmcke

Shahab

et al. 2016

J of Medical Primatology

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“…Lastly, Leydig cells are known to produce TRH, which locally stimulates Leydig cell development and regulates testosterone secretion, independent of thyroid hormone state (Mendis-Handagama & Siril Ariyaratne 2005). Although IGSF1 was expressed only in low levels in Leydig cells, Sertoli cells can influence Leydig cell function through paracrine signaling (Lejeune et al 1992). It is unknown whether TRH receptor (Trhr1) mRNA expression in Leydig cells is decreased in Igsf1…”

Section: Figurementioning

confidence: 99%

Spatial and temporal expression of immunoglobulin superfamily member 1 in the rat

Joustra¹,

Meijer²,

Heinen³

et al. 2015

Journal of Endocrinology

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Loss-of-function mutations in the immunoglobulin superfamily member 1 (IGSF1) gene cause an X-linked syndrome of central hypothyroidism, macroorchidism, variable prolactin and GH deficiency, delayed pubertal testosterone rise, and obesity. To understand the pathophysiology of this syndrome, knowledge on IGSF1's place in normal development is imperative. Therefore, we investigated spatial and temporal protein and mRNA expression of IGSF1 in rats using immunohistochemistry, real-time quantitative PCR (qPCR), and in situ hybridization. We observed high levels of IGSF1 expression in the brain, specifically the embryonic and adult choroid plexus and hypothalamus (principally in glial cells), and in the pituitary gland (PIT1-lineage of GH, TSH, and PRL-producing cells). IGSF1 is also expressed in the embryonic and adult zona glomerulosa of the adrenal gland, islets of Langerhans of the pancreas, and costameres of the heart and skeletal muscle. IGSF1 is highly expressed in fetal liver, but is absent shortly after birth. In the adult testis, IGSF1 is present in Sertoli cells (epithelial stages XIII-VI), and elongating spermatids (stages X-XII). Specificity of protein expression was corroborated with Igsf1 mRNA expression in all tissues. The expression patterns of IGSF1 in the pituitary gland and testis are consistent with the pituitary hormone deficiencies and macroorchidism observed in patients with IGSF1 deficiency. The expression in the brain, adrenal gland, pancreas, liver, and muscle suggest IGSF1's function in endocrine physiology might be more extensive than previously considered.

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“…These SC prosurvival roles may extend to other cells within the testes (such as Leydig cells) or even outside the testes with transplantation or co-transplantation to diseased organs and co-culturing with other cells [4,5,6]. These protective roles of SCs for various cells are most probably based on resistance to apoptotic death signals, but the specific mechanisms of SC survival in normal and stressful environments are not clearly understood.…”

Section: Introductionmentioning

confidence: 99%

“…In consideration of these observations, it can be inferred that autophagy plays prosurvival roles for SCs under basal and pathological conditions. These roles may be essential for spermatogenesis and male fertility based on the various functions of SCs [4,5,6]. …”

Section: Introductionmentioning

confidence: 99%

Upregulated Autophagy in Sertoli Cells of Ethanol-Treated Rats Is Associated with Induction of Inducible Nitric Oxide Synthase (iNOS), Androgen Receptor Suppression and Germ Cell Apoptosis

Horibe

Eid

Ito

et al. 2017

IJMS

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This study was conducted to investigate the autophagic response of Sertoli cells (SCs) to acute ethanol toxicity using in vivo and in vitro models. Adult Wistar rats were intraperitoneally injected with either 5 g/kg ethanol or phosphate-buffered saline (for the control group) and sacrificed 0, 3, 6 and 24 h after injection. Compared to the control group, enhanced germ cell apoptosis was observed in the ethanol-treated rats (ETRs) in association with upregulation of iNOS and reduced expression of androgen receptor protein levels in SCs, which were resistant to apoptosis. Meanwhile, autophagy was upregulated in ETR SCs (peaking at 24 h) compared to the control group, as evidenced by transcription factor EB (TFEB) nuclear translocation, enhanced expression of microtubule-associated protein 1 light chain3-II (LC3-II), lysosome-associated membrane protein-2 (LAMP-2), pan cathepsin protein levels and reduced expression of p62. This upregulation of SC autophagy was confirmed ultrastructurally by enhanced formation of autophagic vacuoles and by immunofluorescent double labelling of autophagosomal and lysosomal markers. Study of cultured SCs confirmed enhanced autophagic response to ethanol toxicity, which was cytoprotective based on decreased viability of SCs upon blocking autophagy with 3-methyladenine (3-MA). The results highlighted the molecular mechanisms of prosurvival autophagy in ETR SCs for the first time, and may have significant implications for male fertility.

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The paracrine role of Sertoli cells on Leydig cell function

Cited by 16 publications

References 60 publications

Immunocytochemical localization of kisspeptin and kisspeptin receptor in the primate testis

Immunocytochemical localization of kisspeptin and kisspeptin receptor in the primate testis

Spatial and temporal expression of immunoglobulin superfamily member 1 in the rat

Upregulated Autophagy in Sertoli Cells of Ethanol-Treated Rats Is Associated with Induction of Inducible Nitric Oxide Synthase (iNOS), Androgen Receptor Suppression and Germ Cell Apoptosis

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