The parabasalid symbiont community of Heterotermes aureus: Molecular and morphological characterization of four new species and reestablishment of the genus Cononympha

Jasso‐Selles, Daniel E.; Martini, Francesca; Freeman, Katalina D; Garcia, Mikaela D.; Merrell, Trevor L.; Scheffrahn, Rudolf H.; Gile, Gillian H.

doi:10.1016/j.ejop.2017.09.001

Cited by 20 publications

(37 citation statements)

References 41 publications

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“…; Jasso‐Selles et al. ; Koidzumi ). The evolutionary relationships among these taxa are unclear because morphology and ultrastructure are understudied in this group and molecular data are currently available only for the genera Spirotrichonympha , Holomastigotoides , and Cononympha (Gile et al.…”

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confidence: 99%

Molecular Identity of Holomastigotes (Spirotrichonymphea, Parabasalia) with Descriptions of Holomastigotes flavipes n. sp. and Holomastigotes tibialis n. sp.

Taerum

Jasso‐Selles

Wilson

et al. 2019

J Eukaryotic Microbiology

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Holomastigotes is a protist genus (Parabasalia: Spirotrichonymphea) that resides in the hindguts of “lower” termites. It can be distinguished from other parabasalids by spiral flagellar bands that run along the entire length of the cell, an anterior nucleus, a reduced or absent axostyle, the presence of spherical vesicles inside the cells, and the absence of ingested wood particles. Eight species have been described based on their morphology so far, although no molecular data were available prior to this study. We determined the 18S rRNA gene sequences of Holomastigotes from the hindguts of Hodotermopsis sjostedti, Reticulitermes flavipes, Reticulitermes lucifugus, and Reticulitermes tibialis. Phylogenetic analyses placed all sequences in an exclusive and well‐supported clade with the type species, Holomastigotes elongatum from R. lucifugus. However, the phylogenetic position of Holomastigotes within the Spirotrichonymphea was not resolved. We describe two new species, Holomastigotes flavipes n. sp. and Holomastigotes tibialis n. sp., inhabiting the hindguts of R. flavipes and R. tibialis, respectively.

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confidence: 99%

Molecular Identity of Holomastigotes (Spirotrichonymphea, Parabasalia) with Descriptions of Holomastigotes flavipes n. sp. and Holomastigotes tibialis n. sp.

Taerum

Jasso‐Selles

Wilson

et al. 2019

J Eukaryotic Microbiology

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“…Termite identification was confirmed by sequencing the mitochondrial 16S ribosomal RNA gene (mt16S), as previously described (Jasso‐Selles et al. ). PCR was performed using Econotaq Plus Green PCR Master Mix (Lucigen, Middleton, WI) and primers LR‐N 5′‐CGC CTG TTT ATC AAA AAC AT‐3′ and LR‐J 5′‐TTA CGC TGT TAT CCC TAA‐3′ (Kambhampati and Smith ; Simon et al.…”

Section: Methodsmentioning

confidence: 99%

“…Each single cell was washed twice in fresh Ringer's buffer and ejected into a 0.5 ml tube for DNA extraction and purification as previously described (Jasso‐Selles et al. ).…”

Section: Methodsmentioning

confidence: 99%

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Molecular Phylogenetic Position of Hoplonympha natator (Trichonymphea, Parabasalia): Horizontal Symbiont Transfer or Differential Loss?

Mee

Gaylor

Jasso‐Selles

et al. 2019

J Eukaryotic Microbiology

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Hoplonympha natator is an obligate symbiont of Paraneotermes simplicicornis (Kalotermitidae), from southwestern North America. Another Hoplonympha species inhabits Hodotermopsis sjostedti (Archotermopsidae), from montane Southeast Asia. The large phylogenetic and geographical distance between the hosts makes the distribution of Hoplonympha puzzling. Here, we report the phylogenetic position of H. natator from P. simplicicornis through maximum likelihood and Bayesian analysis of 18S rRNA genes. The two Hoplonympha species form a clade with a deep node, making a recent symbiont transfer unlikely. The distribution of Hoplonympha may be due to an ancient transfer or strict vertical inheritance with differential loss from other hosts.

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“…We support the use of images when specimens are lost or cannot be retained. For example, protist taxonomy benefits from ICZN (2017) when a novel cell is photographed and then must be destroyed to obtain genomic sequences (Jasso-Selles et al 2017). Because termites have three morphologically distinct caste groups (soldier, imago, worker) the type is selected from the available caste that most distinguishes it from congeners.…”

Section: Discussionmentioning

confidence: 99%

Described from images: The remarkable soldier of Procryptotermes dioscurae Harris (Isoptera: Kalotermitidae) from Socotra Island

Scheffrahn

Krecek

2017

Zootaxa

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Procryptotermes Holmgren is a modestly diverse kalotermitid genus endemic to numerous islands and archipelagos of the world’s tropical oceans (Scheffrahn & Křeček 2001). Mainland records from southern India (Bose 1979, Roonwal & Chhotani 1963, Thakur 1975), Queensland Australia (Watson and Abbey 1993), and the Yucatan Peninsula (Scheffrahn and Křeček 2001), also approximate oceanic coastlines. The soldier of Procryptotermes is distinguished from most other kalotermitids by long, sickle-shaped mandibles and a rather weak or absent frontal flange or protuberance (Krishna 1961). The winged imago of Procryptotermes is similar to that of Cryptotermes Banks in which the median vein is unsclerotized and intersects the radial sector near mid-wing. Of the fourteen described species of Procryptotermes (Scheffrahn & Křeček 2001), only one species, P. dioscurae Harris 1954, lacks a description of the soldier caste. It was described from three winged imagos collected from lights on Socotra Island, Yemen. Unlike soldiers, the imago caste is not present in the colony year round and often lacks species-level diagnostic characters. Therefore, the description of the soldier caste is highly desirable for most termite species.

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The parabasalid symbiont community of Heterotermes aureus: Molecular and morphological characterization of four new species and reestablishment of the genus Cononympha

Cited by 20 publications

References 41 publications

Molecular Identity of Holomastigotes (Spirotrichonymphea, Parabasalia) with Descriptions of Holomastigotes flavipes n. sp. and Holomastigotes tibialis n. sp.

Molecular Identity of Holomastigotes (Spirotrichonymphea, Parabasalia) with Descriptions of Holomastigotes flavipes n. sp. and Holomastigotes tibialis n. sp.

Molecular Phylogenetic Position of Hoplonympha natator (Trichonymphea, Parabasalia): Horizontal Symbiont Transfer or Differential Loss?

Described from images: The remarkable soldier of Procryptotermes dioscurae Harris (Isoptera: Kalotermitidae) from Socotra Island

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