The pan HLA DR-binding epitope improves adjuvant-assisted immunization with a recombinant protein containing a malaria vaccine candidate

Rosa, Daniela Santoro; Tzelepis, Fanny; Cunha, Mariana; Soares, Irene da Silva; Rodrigues, Maurício M.

doi:10.1016/j.imlet.2004.01.006

Cited by 63 publications

(85 citation statements)

References 20 publications

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“…Some studies revealed that PADRE peptide is capable of attaching to the alternates of human MHC class II molecules DR and stimulate helper-mediated immune responses in human species, as well as mice. It can also enhance the potency of vaccines in preclinical models (33,34). Therefore, in the present study, the sequence of tPA and PADRE were added respectively after the Kozak sequence to the N-terminal sequence of truncated ORF2 (aa 112 -607) gene.…”

Section: Discussionmentioning

confidence: 99%

Cloning and Expression of a Secretory form of Truncated ORF2 (aa 112-607) from Hepatitis E Virus in the pVAX1 Vector

Makvandi

Taherkhani

Azizolahi

2017

Jundishapur J Microbiol

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Background: The hepatitis E virus (HEV) accounts for the hepatitis E infection with a high mortality rate in pregnant women. Therefore, the design of the novel and effective vaccines seems essential and the DNA vaccine approach could be useful to achieve this anticipated goal. Objectives: The aim of this study was the cloning of a secretory form of truncated open reading frame 2 (ORF2) of HEV containing amino acids (aa) 112 -607 into the eukaryotic expression pVAX1 Vector and evaluating of the expression of this recombinant protein in eukaryotic cells. Methods: The truncated ORF2 gene (aa 112 -607) was cloned in the pVAX1 plasmid by restriction enzyme digest and confirmed by digestion and sequencing. Then, the recombinant plasmid was transfected into eukaryotic cells to express the recombinant protein.The expressed protein in the cell lysate and supernatant was evaluated by immunofluorescence assay (IFA) and western blot assay. Results: Colony polymerase chain reactions (PCR), restriction enzyme digestion, along with DNA sequencing of the recombinant plasmid were performed for confirmation of cloning tPA-PADRE-truncated ORF2 gene (aa 112 -607) into pVAX1 eukaryotic expression vector. The appearance of the truncated ORF2 protein (56 kDa) in the eukaryotic cells was accepted by the western blot assay, reverse transcription polymerase chain reaction (RT-PCR) method, as well as IFA. Conclusions: All outcomes of the present research showed that pVAX1-tPA-PADRE-truncated ORF2 (aa 112 -607, 56 kDa) recombinant plasmid was able to express truncated ORF2 from HEV as a potential candidate vaccine.

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Section: Discussionmentioning

confidence: 99%

Cloning and Expression of a Secretory form of Truncated ORF2 (aa 112-607) from Hepatitis E Virus in the pVAX1 Vector

Makvandi

Taherkhani

Azizolahi

2017

Jundishapur J Microbiol

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“…9 In vitro, PADRE induces CD4 þ T cell responses in virtually all individuals. 8 It has been used as an adjuvant in vaccination studies in cervical carcinoma, 10 malaria, 11 CMV, 12 polio 13 and Alzheimers disease. 14 …”

Section: Introductionmentioning

confidence: 99%

Clinical evaluation of BCR-ABL peptide immunisation in chronic myeloid leukaemia: results of the EPIC study

et al. 2007

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“…Estudos desenvolvidos por nossa equipe em camundongos mostram uma intensa estimulação de células T em resposta ao re-estímulo in vitro com a PvMSP119-PADRE através do ensaio proliferação celular por incorporação com 3H-Timidina, sem comprometer a resposta de anticorpos (Cunha et al, 2001, Rosa et al, 2004.…”

Section: Msp119unclassified

“…Estudos anteriores demonstraram que a proteína MSP119 ligada ao epítopo PADRE é altamente imunogênica e capaz de estimular proliferação in vitro de células T de camundongos C57BL/6 imunizados com MSP119-PADRE na presença de um adjuvante forte, ACF/AIF (Rosa et al, 2004). No presente trabalho, não foi possível observar proliferação de células T no experimento de imunização com a coadministração dos 3 antígenos na presença de AIF.…”

Section: Avaliação Da Resposta Proliferativa Induzida Pela Imunizaçãounclassified

“…No Brasil, o P. vivax é a espécie mais frequente, responsável por 87% dos casos (WHO, 2012), e apesar de poucos, alguns relatos de complicações tornam-se preocupante (Alexandre et al, 2010, Raposo et al, 2013 Ao longo desses anos, nosso grupo vem estudando a imunogenicidade de várias proteínas recombinantes de P. vivax. Dentre elas, destacam-se as proteínas derivadas de AMA1, MSP1 e MSP3, bastante exploradas em estudos pré-clínicos com diferentes formulações de adjuvantes (Cunha et al, 2001;Rosa et al, 2004;Bargieri et al, 2008;Múfalo et al, 2008, Gentil et al, 2010Vicentin, 2012;Bitencourt et al, 2013 (Cunha et al, 2001;Rosa et al, 2004).…”

Section: Efeito Comparativo Da Imunização De Camundongos C57bl/6 E Baunclassified

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Caracterização da resposta imune celular induzida pela imunização experimental com antígenos recombinantes de Plasmodium vivax

Casale¹

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The pan HLA DR-binding epitope improves adjuvant-assisted immunization with a recombinant protein containing a malaria vaccine candidate

Cited by 63 publications

References 20 publications

Cloning and Expression of a Secretory form of Truncated ORF2 (aa 112-607) from Hepatitis E Virus in the pVAX1 Vector

Cloning and Expression of a Secretory form of Truncated ORF2 (aa 112-607) from Hepatitis E Virus in the pVAX1 Vector

Clinical evaluation of BCR-ABL peptide immunisation in chronic myeloid leukaemia: results of the EPIC study

Caracterização da resposta imune celular induzida pela imunização experimental com antígenos recombinantes de Plasmodium vivax

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