The pan-cancer analysis identified DIAPH3 as a diagnostic biomarker of clinical cancer

Abstract

Objective: This study aimed to determine prognostic biomarkers of cervical cancer by pan-cancer analysis. Materials and Methods: Common differentially expressed genes in Gene Expression Omnibus and The Cancer Genome Atlas (TCGA) database were demonstrated using R software analysis, and these genes were enriched by the Kyoto Encyclopedia of Genes and Genomes and Gene Ontology. Genes with prognostic value were identified by least absolute contraction and selection regression, Cox regression, and survival analysis, and pan-cancer analysis was conducted using the Tumor Immune Estimation Resource database and TCGA database. Western blot, qRT-PCR, and immunohistochemistry were used to preliminarily verify its expression in cervical cancer (S1). Results: The prognostic marker Diaphanous Related Formin 3 (DIAPH3) was obtained from us. The enrichment analysis revealed that DIAPH3 was involved in tumor proliferation, invasion, and inflammation. The pan-cancer analysis revealed that it was highly expressed in various cancers. Immune infiltration analysis revealed that its expression was related to B cells, effector T cells, and macrophage infiltration; however, immune checkpoint correlation analysis and tumor mutation burden analysis revealed the correlation between gene expression and immunotherapy. The expression of DIAPH3 in cervical cancer was significantly different from that in normal cervical tissues. Conclusion: The expression of DIAPH3 in cervical cancer was significantly increased, which may be related to the proliferation, metastasis, immune invasion, and immunotherapy of cervical cancer.

Unveiling novel cell clusters and biomarkers in glioblastoma and its peritumoral microenvironment at the single-cell perspective

“…miRNA-363-3p overexpression inhibits proliferation, invasion and metastasis of thyroid cancer cells, which is reversed by SYT1 overexpression [ 30 ]. Increased expression of DIAPH3 in cervical cancer may be associated with proliferation of cervical cancer [ 31 ]. DIAPH3 expression is also associated with low survival of osteosarcoma patients and can promote proliferation and metastasis of osteosarcoma [ 32 ].…”

mDia2 is an important mediator of MRTF-A-dependent regulation of breast cancer cell migration

“…A number of cancer-related studies have previously documented a role of DIAPH3 in modulating tumor cell aggressiveness albeit in a context-and study-specific manner. For example, pan-cancer analyses revealed that DIAPH3 is upregulated in multiple types of human cancer (breast, cervical, head and neck squamous carcinoma, glioblastoma and several others) and serves as a prognostic manner in certain cancers (Chen et al, 2023). DIAPH3 expression is upregulated in osteosarcoma, and silencing DIAPH3 expression suppresses proliferation, migration/invasion and metastasis of osteosarcoma cells (Zhang et al, 2021).…”