The p27cip/kip ortholog<i>dacapo</i>maintains the<i>Drosophila</i>oocyte in prophase of meiosis I

Hong, Amy; Lee-Kong, Steven A.; Iida, Takako; Sugimura, Isamu; Lilly, Mary A.

doi:10.1242/dev.00352

Cited by 76 publications

(83 citation statements)

References 46 publications

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“…The following antibodies were used (dilutions noted in parentheses): rabbit anti-GFP (1:1000) (Molecular Probes), mouse monoclonal anti-Orb 6H4 (1:10), anti-Sxl (1:10), anti-Cyclin A (1:10), anti-Cyclin B (1:20) and anti-BamC A7 (1:10) (Developmental Studies Hybridoma Bank, Iowa); rabbit polyclonal anti-Spectrin (1:1000) (Byers et al, 1987) (a gift from Ron Dubreuil); rabbit anti-Nanos (a gift from Akira Nakamura); rabbit polyclonal anti-C(3)G (1:3000) (Hong et al, 2003) and anti-Bruno (1:5000) (gifts from Mary Lilly); goat anti-Vasa (1:200) (Santa Cruz Biotechnology); and rabbit polyclonal anti-Rbp9 (1:5000) (a gift from J. Kim-Ha). Fluorescence-conjugated secondary antibodies were used at a 1:200 dilution.…”

Section: Immunohistochemistry and In Situ Hybridizationmentioning

confidence: 99%

DrosophilaAtaxin 2-binding protein 1 marks an intermediate step in the molecular differentiation of female germline cysts

Tastan

Maines

et al. 2010

Development

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SUMMARYIn the Drosophila ovary, extrinsic signaling from the niche and intrinsic translational control machinery regulate the balance between germline stem cell maintenance and the differentiation of their daughters. However, the molecules that promote the continued stepwise development of ovarian germ cells after their exit from the niche remain largely unknown. Here, we report that the early development of germline cysts depends on the Drosophila homolog of the human ataxin 2-binding protein 1 (A2BP1) gene. Drosophila A2BP1 protein expression is first observed in the cytoplasm of 4-, 8-and 16-cell cysts, bridging the expression of the early differentiation factor Bam with late markers such as Orb, Rbp9 and Bruno encoded by arrest. The expression of A2BP1 is lost in bam, sans-fille (snf) and mei-P26 mutants, but is still present in other mutants such as rbp9 and arrest. A2BP1 alleles of varying strength produce mutant phenotypes that include germline counting defects and cystic tumors. Phenotypic analysis reveals that strong A2BP1 alleles disrupt the transition from mitosis to meiosis. These mutant cells continue to express high levels of mitotic cyclins and fail to express markers of terminal differentiation. Biochemical analysis reveals that A2BP1 isoforms bind to each other and associate with Bruno, a known translational repressor protein. These data show that A2BP1 promotes the molecular differentiation of ovarian germline cysts.

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Section: Immunohistochemistry and In Situ Hybridizationmentioning

confidence: 99%

DrosophilaAtaxin 2-binding protein 1 marks an intermediate step in the molecular differentiation of female germline cysts

Tastan

Maines

et al. 2010

Development

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“…CycE overexpressions were carried out using a line harbouring two copies of the UAS-CycE construct, one on the second and one on the third chromosome, respectively. The dap 4 is a null allele of dap (Hong et al, 2003). The A101 lines carry a P(LacZ, ry+) enhancer trap of neuralized and specifically expresses nuclear β-gal in pI and its progeny cells (Usui and Kimura, 1993).…”

Section: Fly Strainsmentioning

confidence: 99%

“…In an attempt to override the Dap-mediated inhibition, we analysed the G1-phase in pIIIb in a null dap background using the dap 4 strain (Hong et al, 2003) in which some homozygous escapers are viable until the pupal stage. Short duration BrdU incorporations were performed to analyse the BrdU incorporation pattern in three-cell clusters.…”

Section: The Delayed Entry Into S-phase In Piiib Cells Arises From Damentioning

confidence: 99%

Cell cycle diversity involves differential regulation of Cyclin E activity in theDrosophilabristle cell lineage

2005

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In the Drosophila bristle lineage, five differentiated cells arise from a precursor cell after a rapid sequence of asymmetric cell divisions (one every 2 hours). We show that, in mitotic cells, this rapid cadence of cell divisions is associated with cell cycles essentially devoid of the G1-phase. This feature is due to the expression of Cyclin E that precedes each cell division, and the differential expression of the S-transition negative regulator, Dacapo. Thus, apart from endocycles (G/S), which occurred in two out of five terminal cells, two other cell cycles coexist in this lineage: (1) an atypical cell cycle (S/G2/M), in which the S-phase is initiated during the preceding telophase; and (2) a canonical cell cycle (G1/S/G2/M) with a brief G1 phase. These two types of cell cycle result from either the absence or very transient expression of Dap, respectively. Finally, we show that the fate determinant factor, Tramtrack, downregulates Cyclin E expression and is probably involved in the exit of the cells from the cell cycle.

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“…Increasing CycE/Cdk2 kinase activity by removing the Cdk-inhibitor dacapo directs oocytes to take on nurse cell characteristics. A mutation that reduces CycE/Cdk2 kinase activity levels results in more cells with oocyte character (Hong et al, 2003;Lilly and Spradling, 1996). Study of bag of marbles (bam) further illustrates the close relationship between cell cycle control and differentiation in the female germ line.…”

Section: Introductionmentioning

confidence: 99%

twin, aCCR4homolog, regulates cyclin poly(A) tail length to permitDrosophilaoogenesis

et al. 2005

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Cyclins regulate progression through the cell cycle. Control of cyclin levels is essential in Drosophila oogenesis for the four synchronous divisions that generate the 16 cell germ line cyst and for ensuring that one cell in each cyst, the oocyte, is arrested in meiosis, while the remaining fifteen cells become polyploid nurse cells. Changes in cyclin levels could be achieved by regulating transcription, translation or protein stability. The proteasome limits cyclin protein levels in the Drosophila ovary, but the mechanisms regulating RNA turnover or translation remain largely unclear. Here, we report the identification of twin, a homolog of the yeast CCR4 deadenylase. We show that twin is important for the number and synchrony of cyst divisions and oocyte fate. Consistent with the deadenylase activity of CCR4 in yeast, our data suggest that Twin controls germ line cyst development by regulating poly(A) tail lengths of several targets including Cyclin A (CycA) RNA. twin mutants exhibit very low expression of Bag-of-marbles (Bam), a regulator of cyst division, indicating that Twin/Ccr4 activity is necessary for wild-type Bam expression. Lowering the levels of CycA or increasing the levels of Bam suppresses the defects we observe in twin ovaries, implicating CycA and Bam as downstream effectors of Twin. We propose that Twin/Ccr4 functions during early oogenesis to coordinate cyst division, oocyte fate specification and egg chamber maturation.

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The p27cip/kip orthologdacapomaintains theDrosophilaoocyte in prophase of meiosis I

Cited by 76 publications

References 46 publications

DrosophilaAtaxin 2-binding protein 1 marks an intermediate step in the molecular differentiation of female germline cysts

DrosophilaAtaxin 2-binding protein 1 marks an intermediate step in the molecular differentiation of female germline cysts

Cell cycle diversity involves differential regulation of Cyclin E activity in theDrosophilabristle cell lineage

twin, aCCR4homolog, regulates cyclin poly(A) tail length to permitDrosophilaoogenesis

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