The p110 Isoform of the CDP/Cux Transcription Factor Accelerates Entry into S Phase

Sansregret, Laurent; Goulet, Brigitte; Harada, Ryuhei; Wilson, B. J.; Leduy, Lam; Bertoglio, Jacques; Nepveu, Alain

doi:10.1128/mcb.26.6.2441-2455.2006

Cited by 61 publications

(98 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using transcription and cellbased assays, a role for p110 CUX1 was shown in many cellular processes, notably in cell cycle progression and cell proliferation 21,22 , strengthening of the spindle assembly checkpoint 19 , establishment of a transcriptional programme that enables efficient DNA damage responses 23 , and cell migration and invasion 13,42 . In addition, from RNA interference (RNAi)-mediated knockdown and genetic inactivation, CUX1 was shown to be required for the resistance to apoptotic signals in pancreatic cancer cells 14 , the repression cytokine genes associated with M1 macrophages 43 , and dendrite branching and spine development in cortical neurons 34 .…”

Section: The Knudson Two-hit Modelmentioning

confidence: 99%

“…However, some observations are relevant to the role of CUX1 in cancer. Mouse embryonic fibroblasts (MEFs) that were derived from Cux1 −/− mice showed a longer G1 phase and proliferated more slowly than their wild-type counterparts 21 . Myeloid hyperplasia was observed in bone marrow, the spleen and the peripheral blood of Cux1 −/− mice, an observation that fits well with frequent CUX1 LOH reported in MPDs 51 .…”

Section: The Knudson Two-hit Modelmentioning

confidence: 99%

“…Many transcriptional targets and cellular functions of CUX1 readily suggest mechanisms by which increased p110 or p75 CUX1 expression might promote tumour development and progression, including acceleration of S phase entry 21,22,41,62,63 , stimulation of cell migration and invasion 13,42,[64][65][66] , resistance to apoptosis 14 , and promotion of bipolar mitosis in the presence of supernumerary centrosomes 19 (reviewed in REF. 67).…”

Section: Mechanisms Of Action In Cancermentioning

confidence: 99%

“…Certainly, the idea that CUX1 represses genes that are involved in cell cycle progression runs contrary to the bulk of the evidence showing that CUX1 stimulates expression of histone genes and many genes involved in DNA replication, while repressing expression of the cyclin-dependent kinase inhibitors p21 and p27 (REFS 15,41,60,62,(68)(69)(70)(71)(72)(73)(74)(75). Moreover, in cell-based assays, MEFs from Cux1-knockout mice showed a longer G1 phase and proliferated more slowly than their wild-type counterparts; whereas, in many cell types, ectopic expression of p110 CUX1 accelerated S phase entry and stimulated proliferation 21,22 .…”

Section: Functions Of Cux1 That Suppress Tumour Developmentmentioning

confidence: 99%

“…Cell-based assays have shown transcriptional roles for CUX1 in cell cycle progression 21,22 , DNA damage responses 23 , and resistance to apoptotic signals 14 and other pathways (FIG. 2).…”

mentioning

confidence: 99%

See 4 more Smart Citations

CUX1, a haploinsufficient tumour suppressor gene overexpressed in advanced cancers

2014

Self Cite

View full text Add to dashboard Cite

Section: The Knudson Two-hit Modelmentioning

confidence: 99%

Section: The Knudson Two-hit Modelmentioning

confidence: 99%

Section: Mechanisms Of Action In Cancermentioning

confidence: 99%

Section: Functions Of Cux1 That Suppress Tumour Developmentmentioning

confidence: 99%

“…Cell-based assays have shown transcriptional roles for CUX1 in cell cycle progression 21,22 , DNA damage responses 23 , and resistance to apoptotic signals 14 and other pathways (FIG. 2).…”

mentioning

confidence: 99%

See 3 more Smart Citations

CUX1, a haploinsufficient tumour suppressor gene overexpressed in advanced cancers

2014

Self Cite

View full text Add to dashboard Cite

Developmental microRNA expression profiling of murine embryonic orofacial tissue

Mukhopadhyay

Brock

Pihur

et al. 2010

Birth Defects Research

View full text Add to dashboard Cite

BACKGROUND Orofacial development is a multifaceted process involving precise, spatio-temporal expression of a panoply of genes. MicroRNAs (miRNAs), the largest family of noncoding RNAs involved in gene silencing, represent critical regulators of cell and tissue differentiation. MicroRNA gene expression profiling is an effective means of acquiring novel and valuable information regarding the expression and regulation of genes, under the control of miRNA, involved in mammalian orofacial development. METHODS To identify differentially expressed miRNAs during mammalian orofacial ontogenesis, miRNA expression profiles from gestation day (GD) -12, -13 and -14 murine orofacial tissue were compared utilizing miRXplore microarrays from Miltenyi Biotech. Quantitative real-time PCR was utilized for validation of gene expression changes. Cluster analysis of the microarray data was conducted with the clValid R package and the UPGMA clustering method. Functional relationships between selected miRNAs were investigated using Ingenuity Pathway Analysis. RESULTS Expression of over 26% of the 588 murine miRNA genes examined was detected in murine orofacial tissues from GD-12–GD-14. Among these expressed genes, several clusters were seen to be developmentally regulated. Differential expression of miRNAs within such clusters were shown to target genes encoding proteins involved in cell proliferation, cell adhesion, differentiation, apoptosis and epithelial-mesenchymal transformation, all processes critical for normal orofacial development. CONCLUSIONS Using miRNA microarray technology, unique gene expression signatures of hundreds of miRNAs in embryonic orofacial tissue were defined. Gene targeting and functional analysis revealed that the expression of numerous protein-encoding genes, crucial to normal orofacial ontogeny, may be regulated by specific miRNAs.

show abstract

Ectopic expression of Cux1 is associated with reduced p27 expression and increased apoptosis during late stage cyst progression upon inactivation of Pkd1 in collecting ducts

Paul

Vassmer

Taylor

et al. 2011

Developmental Dynamics

View full text Add to dashboard Cite

Polycystic kidney diseases (PKD) are inherited disorders characterized by fluid-filled cysts primarily in the kidneys. We previously reported differences between the expression of Cux1, p21 and p27 in the cpk and Pkd1 null mouse models of PKD. Embryonic lethality of Pkd1 null mice limits its study to early stages of kidney development. Therefore, we examined mice with a collecting duct specific deletion in the Pkd1 gene. Cux1 was ectopically expressed in the cyst lining epithelial cells of newborn, P7 and P15 Pkd1CD mice. Cux1 expression correlated with cell proliferation in early stages of cystogenesis, however, as the disease progressed, fewer cyst lining cells showed increased cell proliferation. Rather, Cux1 expression in late stage cystogenesis was associated with increased apoptosis. Taken together, our results suggest that increased Cux1 expression associated with apoptosis is a common feature of late stage cyst progression in both the cpk and Pkd1CD mouse models of PKD.

show abstract

The p110 Isoform of the CDP/Cux Transcription Factor Accelerates Entry into S Phase

Cited by 61 publications

References 73 publications

CUX1, a haploinsufficient tumour suppressor gene overexpressed in advanced cancers

CUX1, a haploinsufficient tumour suppressor gene overexpressed in advanced cancers

Developmental microRNA expression profiling of murine embryonic orofacial tissue

Ectopic expression of Cux1 is associated with reduced p27 expression and increased apoptosis during late stage cyst progression upon inactivation of Pkd1 in collecting ducts

Contact Info

Product

Resources

About