The P-700-chlorophyl a-protein complex and two major light-harvesting complexes of Acrocarpia paniculata and other brown seaweeds

Barrett, J.; Anderson, Jan M.

doi:10.1016/0005-2728(80)90202-9

Cited by 77 publications

(45 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The absorption maxima of band II at 585,636 and 672 nm, compare closely to those seen in [4] in a chl a/&l c-containing fraction from the brown alga, Acrocarpia paniculata; however, the chl c/chl a ratio in the chl a-chl c-complex from Glenodinium sp. at 4.8/l is much greater than that found in the complex from A. paniculuta (0.33/l).…”

Section: Discussionsupporting

confidence: 57%

A chlorophyll c‐containing pigment—protein complex from the marine dinoflagellate, Glenodinium sp

et al. 1980

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 57%

A chlorophyll c‐containing pigment—protein complex from the marine dinoflagellate, Glenodinium sp

et al. 1980

View full text Add to dashboard Cite

“…Fluorescence emission and excitation at 77" K were recorded as in [ 131. Chl a and chl c were assayed in 80% acetone 1141. Chl a, cfif cl and chl c2 molar ratios were estimated in ethanol by fluorescence spectroscopy [12].…”

Section: Methodsmentioning

confidence: 99%

Isolation of a F694—chlorophylla—protein complex with low fluorescence yield from brown algae

Barrett

Thorne

1980

FEBS Letters

Self Cite

View full text Add to dashboard Cite

The isolation of intrinsic proteins from membranes has been accomplished largely by the use of neutral detergents [l]. Steroidal anionic detergents also have been used for the solubilization of cytochrome oxidase [2,3] cytochromes b 141 and erythrocyte glycoprotein [5 1. Digitonin, which is non-ionic has been used for preparations of PSI and PST1 macrocomplexes [6-83. For the isolation and analysis of the chlorophyll-protein complexes, Triton X-100 and sodium dodecylsulphate have been extensively employed [93. However, the former detergent may cause a blueshift in the chl-protein spectra and the latter agent can cause excessive dissociation of chl from their complexes with polypeptides.The P700 chl a-protein complex and two major light-harvesting complexes have been isolated from brown algae by Triton X-l 00 [lo]. Selective partial extraction of Acrocarpia ~unicu~ta thylakoids by Triton X-100 in low concentration also revealed the presence of a separate chl c?--protein of small AI, and a far-red spectral form of chl a. We have attempted to release selectively intrinsic proteins from the photosynthetic membranes of brown algae by sequential use of steroidal and alkyl detergents. The isolation of several new pigment-protein complexes has been reported in [ 111. This paper described the isolation of a large chl a-protein complex which has absorbance Abbreviations: chl, chiorophyll; PSI and PSII, photosystem I and II; Tricine, ~-~~~~ydroxymethyl)methyl glycine; LDAO, ~~yldimethylam~e oxide; Hepes. 4-(2 hydro~yethyl)-lpiperazine-ethane sulphonic acid t Visitor to Plant Industry, CSIRO, Canberra, to which address correspondence should be sent 24 and fluorescence properties consistent with it being a PSI1 reaction centre complex. Materials and methodsFronds of Acrocarpia paniculata were collected from submerged plants at low-tide off a rock-reef at Guerilla Bay, NSW China ~o~~erson~i was collected from channels between rock-clefts. Both the seaweeds were kept in cold, aerated seawater until used, usually within 24-48 h. Ruptured chloroplasts were prepared from these fronds as in [lo]. The molar ratio of chl a/&l c of these chloroplasts was 4.0-4.8.For selective solubilization of the cl-d-protein complexes, the ruptured chloroplasts (containing 8 mg total chl a t c) were successively extracted with 1% cholate (pH 8.0 and 9.0), 1% deoxycholate (pH 8.0 and 9.0) and finally with 1% LDAO (pH 8.0 and 9.0): all were in 100 mM Tricine buffer (pH 8.0). The weight ratio of cholate to chl a + c (w/w) was 60: 1 at the start. Extraction was at 4OC with stirring for 8 h, and the suspension was then centrifuged at 15 000 X g for 10 mm. The supernatants (40-50 ml) were concentrated to 1 ml using Centriflo CF 25 cones, and the concentrate was applied to a tube of a sucrose density gradient prepared as in [ 121. Centrifugation was carried out in a Beckman SW 41 rotor at 2'C at 273 000 X g for 60 h.Absorption spectra were obtained using a Cary 14 spectrophotometer. Fluorescence emission and excitation at 77" K were recorded as in [ 131. ...

show abstract

“…Although reaction center preparations with PSI photochemical activities have been described from most of these algal groups (1,3,4,15,23), and in some cases PSI1 reaction centers (14), the major light-harvesting pigment-protein complexes are poorly defined with the exception of the water soluble peridinin-Chl aproteins of the dinoflagellates (13,24,25,27).…”

Section: Introductionmentioning

confidence: 99%

“…In part, this is because the biochemistry, genetics, and cell biology of marine algal groups such as the diatoms, chrysophytes, cryptophytes, dinoflagellates, and brown algae are unknown or poorly investigated. Further, the photosynthetic membranes of these algae appear to be far less tractable and more sensitive (cf 1-4, 15, 23) to detergents which have been commonly used in the isolation of green plant pigment-protein complexes (18,31).Although reaction center preparations with PSI photochemical activities have been described from most of these algal groups (1,3,4,15,23), and in some cases PSI1 reaction centers (14), the major light-harvesting pigment-protein complexes are poorly defined with the exception of the water soluble peridinin-Chl aproteins of the dinoflagellates (13,24,25,27). …”

mentioning

confidence: 99%

A Diatom Light-Harvesting Pigment-Protein Complex

Friedman¹,

Alberte²

1984

Plant Physiol.

View full text Add to dashboard Cite

A light-harvesting pigment-protein complex was isolated from the diatom Phaeodactylm ticornntam using the zwitterionic detergent CHAPS (3-(3-cholamidopropyl)dimethyammonioj1-propanesulfonate).Detergent-solubilized membranes were fractionated by sucrose density gradient centrifugation into three components. The medium density fraction contained chlorophyll a, chlorophyll c, and fucoxanthin. This fraction was purified by DEAE-ion exchange chromatography, and contained chlorophyll a, chlorophyll c, and fucoxanthin in a molar ratio of The isolation and characterization of pigment-protein complexes containing the major light-harvesting carotenoids such as fucoxanthin, peridinin, and siphonoxanthin (17) has not met with the same success or attention as the green plant Chl-proteins. In part, this is because the biochemistry, genetics, and cell biology of marine algal groups such as the diatoms, chrysophytes, cryptophytes, dinoflagellates, and brown algae are unknown or poorly investigated. Further, the photosynthetic membranes of these algae appear to be far less tractable and more sensitive (cf 1-4, 15, 23) to detergents which have been commonly used in the isolation of green plant pigment-protein complexes (18,31).Although reaction center preparations with PSI photochemical activities have been described from most of these algal groups (1,3,4,15,23), and in some cases PSI1 reaction centers (14), the major light-harvesting pigment-protein complexes are poorly defined with the exception of the water soluble peridinin-Chl aproteins of the dinoflagellates (13,24,25,27).

show abstract

The P-700-chlorophyl a-protein complex and two major light-harvesting complexes of Acrocarpia paniculata and other brown seaweeds

Cited by 77 publications

References 31 publications

A chlorophyll c‐containing pigment—protein complex from the marine dinoflagellate, Glenodinium sp

A chlorophyll c‐containing pigment—protein complex from the marine dinoflagellate, Glenodinium sp

Isolation of a F694—chlorophylla—protein complex with low fluorescence yield from brown algae

A Diatom Light-Harvesting Pigment-Protein Complex

Contact Info

Product

Resources

About