The Oxylipin Signaling Pathway Is Required for Increased Aphid Attraction and Retention on Virus-Infected Plants

Bera, Sayanta; Blundell, Robert; Liang, Danna; Crowder, David W.; Casteel, Clare L.

doi:10.1007/s10886-020-01157-7

Cited by 21 publications

(14 citation statements)

References 67 publications

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“…After 48 hr, A. pisum were removed by aspiration and plants were grown for 7 days before harvesting. While transcript abundances can vary over time, we waited 7 days to harvest plants as this is the amount of time for PEMV to be detectable and to allow for comparison to other studies that used this time period for analysis (Bera et al, 2020;Chisholm et al, 2018). Each of the treatments [2 soils (rhizobia or control) × 3 A. pisum (viruliferous, sham and none)] had four biological replicates.…”

Section: Analyses Of Transcripts Related To Phytohormone Signallingmentioning

confidence: 99%

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Reciprocal plant‐mediated antagonism between a legume plant virus and soil rhizobia

et al. 2021

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1. Beneficial plant-associated soil microbes can promote plant tolerance to stress and promote nutrient uptake. Yet, the benefits of microbes for plant health can be altered by above-ground stressors like herbivores and pathogens. However, few studies have assessed reciprocal plant-mediated interactions between beneficial soil microbes and multiple above-ground stressors.2. We assessed if soil rhizobia influenced complex interactions among pea host plants, a vector herbivore (aphids) and a plant virus (Pea enation mosaic virus, PEMV). We also examined how aphids and PEMV affected the function of soil rhizobia.3. We show that plants attacked by PEMV produced fewer root nodules and had lower fresh root nodule biomass per g of fresh plant root biomass, and decreased expression of genes associated with nodulation, suggesting PEMV inhibited nitrogen fixation by rhizobia. However, soil rhizobia decreased aphid abundance and PEMV titre on host plants, such that rhizobia decreased the susceptibility of plants to herbivores and pathogens. 4. Assays of amino acids, and gene expression related to hormone signalling, show interactions among rhizobia, plants, aphids and PEMV were mediated by plant defence and nutrients. Viruliferous aphids induced salicylic acid in plants, and salicylic acid suppressed the function of rhizobia. Aphids feeding on plants grown in rhizobia-inoculated soil also obtained fewer essential amino acids than those feeding on plants grown in un-inoculated sterilized soil.5. Mutually antagonistic plant-mediated interactions between soil microbes and above-ground stressors affected plant susceptibility and herbivore nutrient uptake. This suggests ecological effects of soil microbes and above-ground stressors for plant health will likely vary based on multi-trophic plant-mediated interactions among herbivores, pathogens and soil microbes.

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Section: Analyses Of Transcripts Related To Phytohormone Signallingmentioning

confidence: 99%

“…Arabidopsis spp., P. sativum, and other plants (Adio et al, 2011;Bera et al, 2020;Ghazijahani et al, 2014). Twelve replicates were used per treatment.…”

Section: These Concentrations Reflect Levels Used In Experiments Withmentioning

confidence: 99%

Reciprocal plant‐mediated antagonism between a legume plant virus and soil rhizobia

et al. 2021

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“…However, it is unknown if responses of P. sativum differ based on the number of stressors, and their order of attack. Moreover, molecular mechanisms that mediate interactions among these stressors are largely unknown (Chisholm et al, 2019; Bera et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

“…We addressed these knowledge gaps by assessing the response of Pisum sativum plants to attack from a piercing-sucking vector herbivore, the pea aphid ( Acrythosiphon pisum ), a chewing non-vector herbivore, the pea leaf weevil ( Sitona lineatus ), and an aphid-borne pathogen, Pea-enation mosaic virus (PEMV). These organisms co-occur in ecosystems of eastern Washington and northern Idaho, USA, and interactions between them can affect plant traits and signaling pathways affecting insects and pathogens (Chisholm, Eigenbrode, Clark, Basu, & Crowder, 2019; Bera, Blundell, Liang, Crowder, & Casteel, 2020). However, the order in which herbivores and pathogens arrive on hosts, which varies across sites (Chisholm et al, 2019), may impact plant traits and defenses.…”

Section: Introductionmentioning

confidence: 99%

Plant responses to multiple antagonists are mediated by order of attack and phytohormone crosstalk

Basu

Clark

Bera

et al. 2021

Preprint

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Plants are often attacked by multiple antagonists, and traits of the attacking organisms, and their order of arrival onto hosts, may affect plant defenses. However, few studies have assessed how multiple antagonists, and varying attack order, affect plant defense or nutrition. To address this, we assessed defensive and nutritional responses of Pisum sativum plants after attack by a vector herbivore (Acrythosiphon pisum), a non-vector herbivore (Sitona lineatus), and a pathogen (Pea enation mosaic virus, PEMV). We show PEMV-infectious A. pisum induced several pathogen-specific plant defense signals, but these defenses were inhibited when S. lineatus was present in peas infected with PEMV. Sitona lineatus also increased abundance of plant amino acids, but only when they attacked after PEMV-infectious A. pisum. Feeding by S. lineatus also promoted expression of several anti-herbivore defenses, but these defenses were inhibited when PEMV was also present. Our results suggest that diverse communities of biotic antagonists alter defense and nutritional traits of plants through complex pathways that depend on the identity of attackers and their order of arrival onto hosts. Moreover, simply examining the jasmonic and salicylic acid pathways may fail to reveal more complex pathways by which plants respond to biotic stress.

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“…We addressed these knowledge gaps by assessing the response ofPisum sativum plants to attack from a piercing-sucking vector herbivore, the pea aphid (Acrythosiphon pisum ), a chewing non-vector herbivore, the pea leaf weevil (Sitona lineatus ), and an aphid-borne pathogen, Pea-enation mosaic virus (PEMV). These organisms co-occur in ecosystems of eastern Washington and northern Idaho, USA, and interactions between them can affect plant traits and signaling pathways affecting insects and pathogens (Chisholm, Eigenbrode, Clark, Basu, & Crowder, 2019;Bera, Blundell, Liang, Crowder, & Casteel, 2020). However, the order in which herbivores and pathogens arrive on hosts, which varies across sites (Chisholm et al, 2019), may impact plant traits and defenses.…”

Section: Introductionmentioning

confidence: 99%

Plant responses to multiple antagonists are mediated by order of attack and phytohormone crosstalk

Basu

Clark

Bera

et al. 2021

Preprint

View full text Add to dashboard Cite

Plants are often attacked by multiple antagonists, and traits of the attacking organisms, and their order of arrival onto hosts, may affect plant defenses. However, few studies have assessed how multiple antagonists, and varying attack order, affect plant defense or nutrition. To address this, we assessed defensive and nutritional responses of Pisum sativum plants after attack by a vector herbivore (Acrythosiphon pisum), a non-vector herbivore (Sitona lineatus), and a pathogen (Pea enation mosaic virus, PEMV). We show PEMV-infectious A. pisum induced several pathogen-specific plant defense signals, but these defenses were inhibited when S. lineatus was present in peas infected with PEMV. In contrast, feeding by S. lineatus induced anti-herbivore defense signals, but these defenses were enhanced by PEMV. Sitonalineatus also increased abundance of plant amino acids, but only when they attacked after PEMV-infectious A. pisum. Our results suggest that diverse communities of biotic antagonists alter defense and nutritional traits of plants through complex pathways that depend on the identity of attackers and their order of arrival onto hosts. Moreover, we show interactions among a group of biotic stressors can vary along a spectrum from antagonism to enhancement/synergism based on the identity and order of attackers, and these interactions are mediated by a multitude of phytohormone pathways.

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The Oxylipin Signaling Pathway Is Required for Increased Aphid Attraction and Retention on Virus-Infected Plants

Cited by 21 publications

References 67 publications

Reciprocal plant‐mediated antagonism between a legume plant virus and soil rhizobia

Reciprocal plant‐mediated antagonism between a legume plant virus and soil rhizobia

Plant responses to multiple antagonists are mediated by order of attack and phytohormone crosstalk

Plant responses to multiple antagonists are mediated by order of attack and phytohormone crosstalk

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