The Oxygen Radical Scavenger Pyrrolidine Dithiocarbamate Enhances Interleukin-1β-Induced Cyclooxygenase-2 Expression in Cerebral Microvascular Smooth Muscle Cells

Fang, Xiang; Chen, Ping; Moore, Steven A.

doi:10.1006/mvre.2002.2431

Cited by 7 publications

(7 citation statements)

References 31 publications

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“…The blots were then incubated with horseradish peroxidase‐linked donkey anti‐rabbit secondary antibody (1:2500, Santa Cruz Biotechnology). Antibody labelling was detected by ECL 23 …”

Section: Methodsmentioning

confidence: 99%

Enhanced levels of prostaglandin E₂ and matrix metalloproteinase‐2 correlate with the severity of airflow limitation in stable COPD

Chen

Hanaoka

et al. 2008

Respirology

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“…The blots were then incubated with horseradish peroxidase‐linked donkey anti‐rabbit secondary antibody (1:2500, Santa Cruz Biotechnology). Antibody labelling was detected by ECL 23 …”

Section: Methodsmentioning

confidence: 99%

Enhanced levels of prostaglandin E₂ and matrix metalloproteinase‐2 correlate with the severity of airflow limitation in stable COPD

Chen

Hanaoka

et al. 2008

Respirology

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“…To explore the effect of AP‐2α on COX‐2 expression in A549 cells, subconfluent A549 cells (70–80% confluent) in six‐well plates were transfected with various concentrations of the adenovirus vector encoding for the activator protein AP‐2α (AdAP‐2α) for 24 h using Effectene Transfection Reagent, according to the protocol provided by the manufacturer. AP‐2Δ plasmid (1.0 µg) or control plasmid vector AdBgII (1.0 µg) were transfected for 18 h. The cells were then stimulated with IL‐1β for another 6 h 4 …”

Section: Methodsmentioning

confidence: 99%

“…The blots were then incubated with a horseradish peroxidase‐linked donkey anti‐rabbit secondary antibody (1:2500). Antibody labelling was detected by ECL 4 …”

Section: Methodsmentioning

confidence: 99%

“…There are two COX isoforms: COX‐1 is a constitutive form and COX‐2 is an inducible form, which is induced by many cytokines, growth factors and by endotoxin. Increased expression of COX‐2 has been linked to inflammation and cancer 2,4 . Previous studies have shown that asthma and COPD are inflammatory diseases, and that COX‐2 plays an important role in their pathophysiology 5,6 …”

Section: Introductionmentioning

confidence: 99%

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Involvement of PKC, p38 MAPK and AP‐2 in IL‐1β‐induced expression of cyclooxygenase‐2 in human pulmonary epithelial cells

et al. 2006

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“…Protein content was determined by a microbicinchoninic acid assay (Pierce) with bovine serum albumin as standard. Western blot analysis was performed as described previously [6]. Briefly, the protein was separated by electrophoresis on a 10% polyacrylamide gel at 180 V for 45 min.…”

Section: Methodsmentioning

confidence: 99%

Atorvastatin reduces lipopolysaccharide-induced expression of cyclooxygenase-2 in human pulmonary epithelial cells

Duan

Zhao

et al. 2005

Respir Res

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Objective: To explore the effects of atorvastatin on expression of cyclooxygenase-2 (COX-2) in human pulmonary epithelial cells (A549).Methods: A549 cells were incubated in DMEM medium containing lipopolysaccharide (LPS) in the presence or absence of atorvastatin. After incubation, the medium was collected and the amount of prostaglandin E 2 (PGE 2 ) was measured by enzyme-linked immunosorbent assay (ELISA). The cells were harvested, and COX-2 mRNA and protein were analyzed by RT-PCR and western-blot respectively.Results: LPS increased the expression of COX-2 mRNA and production of PGE 2 in a dose-and time-dependent manner in A549. Induction of COX-2 mRNA and protein by LPS were inhibited by atorvastatin in a dose-dependent manner. Atorvastatin also significantly decreased LPS-induced production of PGE 2 . There was a positive correlation between reduced of COX-2 mRNA and decreased of PGE 2 (r = 0.947, P < 0.05). Conclusion:Atorvastatin down-regulates LPS-induced expression of the COX-2 and consequently inhibits production of PGE 2 in cultured A549 cells.

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The Oxygen Radical Scavenger Pyrrolidine Dithiocarbamate Enhances Interleukin-1β-Induced Cyclooxygenase-2 Expression in Cerebral Microvascular Smooth Muscle Cells

Cited by 7 publications

References 31 publications

Enhanced levels of prostaglandin E₂ and matrix metalloproteinase‐2 correlate with the severity of airflow limitation in stable COPD

Enhanced levels of prostaglandin E₂ and matrix metalloproteinase‐2 correlate with the severity of airflow limitation in stable COPD

Involvement of PKC, p38 MAPK and AP‐2 in IL‐1β‐induced expression of cyclooxygenase‐2 in human pulmonary epithelial cells

Atorvastatin reduces lipopolysaccharide-induced expression of cyclooxygenase-2 in human pulmonary epithelial cells

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The Oxygen Radical Scavenger Pyrrolidine Dithiocarbamate Enhances Interleukin-1β-Induced Cyclooxygenase-2 Expression in Cerebral Microvascular Smooth Muscle Cells

Cited by 7 publications

References 31 publications

Enhanced levels of prostaglandin E2 and matrix metalloproteinase‐2 correlate with the severity of airflow limitation in stable COPD

Enhanced levels of prostaglandin E2 and matrix metalloproteinase‐2 correlate with the severity of airflow limitation in stable COPD

Involvement of PKC, p38 MAPK and AP‐2 in IL‐1β‐induced expression of cyclooxygenase‐2 in human pulmonary epithelial cells

Atorvastatin reduces lipopolysaccharide-induced expression of cyclooxygenase-2 in human pulmonary epithelial cells

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Resources

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Enhanced levels of prostaglandin E₂ and matrix metalloproteinase‐2 correlate with the severity of airflow limitation in stable COPD

Enhanced levels of prostaglandin E₂ and matrix metalloproteinase‐2 correlate with the severity of airflow limitation in stable COPD