The OXR domain defines a conserved family of eukaryotic oxidation resistance proteins

Durand, Mathieu; Kolpak, Adrianne L.; Farrell, Timothy M.; Elliott, Nathan; Shao, Wenlin; Brown, Myles; Volkert, Michael R.

doi:10.1186/1471-2121-8-13

Cited by 63 publications

(80 citation statements)

References 36 publications

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“…The TLDc domain is of undetermined function, though a recent paper suggests its involvement in protection from oxidative damage (Durand et al 2007). Moreover, previous studies of the yeast Oxr1 gene, which also contains a TLDc domain, indicate that it functions to protect cells from oxidative damage (Volkert et al 2000, Elliott & Volkert 2004.…”

Section: Discussionmentioning

confidence: 99%

“…The only domain that is common to both Ncoa7 and Ncoa7B is the TLDc, which consists the major portion of Ncoa7B (59-221 aa). This domain is of undetermined function, though a recent paper suggests its involvement in protection from oxidative damage (Durand et al 2007). …”

Section: Resultsmentioning

confidence: 99%

“…From the alignment of TLDc domain containing proteins, the closest relative of NCOA7 is shown to be OXR1, which also has a corresponding, highly related short variant, C7C (Fischer et al 2001). OXR1 has been shown to be localized to the mitochondrion (Elliott & Volkert 2004), while NCOA7 is localized to the nucleus (Durand et al 2007). Of the other family members, KIAA1609 and Figure 5 (A) Multiple alignment of the TLDc domain.…”

Section: Discussionmentioning

confidence: 99%

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Molecular characterization and bioinformatics analysis of Ncoa7B, a novel ovulation-associated and reproduction system-specific Ncoa7 isoform

Shkolnik

Ben‐Dor²,

Galiani³

et al. 2008

Reproduction

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In the present work, we employed bioinformatics search tools to select ovulation-associated cDNA clones with a preference for those representing putative novel genes. Detailed characterization of one of these transcripts, 6C3, by real-time PCR and RACE analyses led to identification of a novel ovulation-associated gene, designated Ncoa7B. This gene was found to exhibit a significant homology to the Ncoa7 gene that encodes a conserved tissue-specific nuclear receptor coactivator. Unlike Ncoa7, Ncoa7B possesses a unique and highly conserved exon at the 5 0 end and encodes a protein with a unique N-terminal sequence. Extensive bioinformatics analysis has revealed that Ncoa7B has one identifiable domain, TLDc, which has recently been suggested to be involved in protection from oxidative DNA damage. An alignment of TLDc domain containing proteins was performed, and the closest relative identified was OXR1, which also has a corresponding, highly related short isoform, with just a TLDc domain. Moreover, Ncoa7B expression, as seen to date, seems to be restricted to mammals, while other TLDc family members have no such restriction. Multiple tissue analysis revealed that unlike Ncoa7, which was abundant in a variety of tissues with the highest expression in the brain, Ncoa7B mRNA expression is restricted to the reproductive system organs, particularly the uterus and the ovary. The ovarian expression of Ncoa7B was stimulated by human chorionic gonadotropin. Additionally, using real-time PCR, we demonstrated the involvement of multiple signaling pathways for Ncoa7B expression on preovulatory follicles.

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Section: Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Molecular characterization and bioinformatics analysis of Ncoa7B, a novel ovulation-associated and reproduction system-specific Ncoa7 isoform

Shkolnik

Ben‐Dor²,

Galiani³

et al. 2008

Reproduction

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“…The NCoA7 protein contains similar functional domains to the mitochondrial OXR1-protein, which is known to reduce oxidative damage in DNA structure. In contrast to OXR-1, NCoA7 is not stress-induced but reacts to estrogens and interacts with the estrogen receptor (10). Given that NCoA7 is not estrogen receptor-specific but binds to other nuclear receptors as well, this coactivator is involved in a variety of other signalling pathways (7,19).…”

Section: Allele Positivity Allele Positivity Genotype Frequencymentioning

confidence: 99%

“…Certain studies have indicated that NCoA7 may be a favourable prognostic indicator for neuroblastomas. On the other hand, other authors identified an isoform that appears to be ovulation-associated and reproduction system-specific; another study found NCoA7 to possess an activity that contributes to oxidation resistance (8,9,10). Given that NCoA7 is expressed in the mammary gland and thus plays a role in the response of this tissue to estrogens, molecular alterations in the NCoA7 protein may affect breast cancer risk.…”

Section: Introductionmentioning

confidence: 99%

A polymorphism in the nuclear receptor coactivator 7 gene and breast cancer susceptibility

et al. 2011

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Abstract. The nuclear receptor coactivator 7 (NCoA7) gene codes for an estrogen receptor-associated protein that plays a significant role in the cellular response to estrogens. Given that NCoA7 is expressed in the mammary gland, alterations in this gene may affect breast cancer risk. In this study, we compared the genotype and allele frequencies of the missense single nucleotide polymorphism (SNP) rs1567, located in the coding region of the NCoA7 gene and resulting in an amino acid exchange from asparagine to glutamine, in 305 women with sporadic breast cancer and 346 women without any malignancy. Statistical analysis of the observed frequencies did not reveal a significant difference between the cancer and control groups, nor did a comparison between histological breast cancer subgroups. In conclusion, the results of our phenotype-genotype association study indicate that NCoA7 SNP rs1567 does not affect breast cancer susceptibility.

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Crystal structure of the TLDc domain of oxidation resistance protein 2 from zebrafish

et al. 2012

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The oxidation resistance proteins (OXR) help to protect eukaryotes from reactive oxygen species. The sole C-terminal domain of the OXR, named TLDc is sufficient to perform this function. However, the mechanism by which oxidation resistance occurs is poorly understood. We present here the crystal structure of the TLDc domain of the oxidation resistance protein 2 from zebrafish. The structure was determined by X-ray crystallography to atomic resolution (0.97Å) and adopts an overall globular shape. Two antiparallel β-sheets form a central β-sandwich, surrounded by two helices and two one-turn helices. The fold shares low structural similarity to known structures.

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The OXR domain defines a conserved family of eukaryotic oxidation resistance proteins

Cited by 63 publications

References 36 publications

Molecular characterization and bioinformatics analysis of Ncoa7B, a novel ovulation-associated and reproduction system-specific Ncoa7 isoform

Molecular characterization and bioinformatics analysis of Ncoa7B, a novel ovulation-associated and reproduction system-specific Ncoa7 isoform

A polymorphism in the nuclear receptor coactivator 7 gene and breast cancer susceptibility

Crystal structure of the TLDc domain of oxidation resistance protein 2 from zebrafish

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