The oxidative stress response of the filamentous yeastTrichosporon cutaneumR57 to copper, cadmium and chromium exposure

Abstract

Despite the intensive research in the past decade on the microbial bioaccumulation of heavy metals, the significance of redox state for oxidative stress induction is not completely clarified. In the present study, we examined the effect of redox-active (copper and chromium) and redox-inactive (cadmium) metals on the changes in levels of oxidative stress biomarkers and antioxidant enzyme defence in Trichosporon cutaneum R57 cells. This filamentous yeast strain showed significant tolerance and bioaccumulation capability of heavy metals. Our findings indicated that the treatment by both redox-active and redox-inactive heavy metal induced oxidative stress events. Enhanced concentrations of Cu2+, Cr6+ and Cd2+ caused acceleration in the production of reactive oxygen species (ROS), increase in the level of oxidatively damaged proteins and accumulation of reserve carbohydrates (glycogen and trehalose). Cell response against heavy metal exposure also includes elevation in the activities of antioxidant enzymes, superoxide dismutase and catalase, which are key enzymes for directly scavenging of ROS. Despite the mentioned changes in the stress biomarkers, T. cutaneum did not show a significant growth diminution. Probably, activated antioxidant defence contributes to the yeast survival under conditions of heavy metal stress.

Cellular Response to Cu‐ and Zn‐Induced Oxidative Stress in Aspergillus fumigatus Isolated From Polluted Soils in Bulgaria

“…The present results about the increased level of carbonylation in Cu‐ and Zn‐exposed A. fumigatus are in accordance with previously published studies reporting similar data in fungal cells treated with heavy metals, such as copper, zinc, cadmium, etc. . As shown in Fig.…”

“…The increase in glycogen and trehalose levels in fungal response to adverse environmental conditions has been recorded as a cellular response to induced oxidative stress . Furthermore, the prevalence of heavy metals in the living environment instigates oxidative stress . Hence, it is not surprising to find enhanced levels of reserve carbohydrates in A. fumigatus cells treated with Cu and Zn ions.…”

“…Similar results have been reported for Phanerochaete chrysosporium treated with Cu and Zn ions , P. thomii treated with Cu ions , etc. The increasing trend is due to an elevated rate of production of ROS in the presence of those metal ions . The antioxidant enzymes present in fungal cells play an important role, resisting oxidative damage, which leads to adaptation and ultimate survival under heavy metal stress conditions .…”

Cadmium-Induced Cell Homeostasis Impairment is Suppressed by the Tor1 Deficiency in Fission Yeast

“…Additionally, our data clearly, although surprisingly, demonstrate that the Tor1 deficiency significantly protects cells against Cd-induced oxidative stress ( Figure 4 ). The redox-inactive Cd was reported to indirectly accelerate the production of reactive oxygen species (ROS) · O 2 − and H 2 O 2 in yeasts in a dose-dependent manner, resulting in the activation of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) [ 35 , 36 ]. In our study, no difference was observed in the catalase activity of both tested yeast strains before Cd treatment.…”

The Ustilago maydis null mutant strains of the RNA-binding protein UmRrm75 accumulate hydrogen peroxide and melanin

“…In fungi, like in many other aerobic organisms, one of the first cell detoxification responses is against ROS accumulation, which includes an increase in the activities of the principal antioxidant enzymes, such as superoxide dismutase (SOD) and catalase (CAT), which play key roles in ROS scavenging 50–52 . Herein, we reported that CAT activity was higher in the 1/46 mutant strain than FB2 parental strain.…”