The oxidative activity of particulate fractions from mosquitoes

Gonda, O.; Traub, A.; Avi‐Dor, Y.

doi:10.1042/bj0670487

Cited by 42 publications

(16 citation statements)

References 15 publications

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“…This strongly indicates that lipids are not suitable substrates to support respiration in A . aegypti flight muscle, as previously suggested [ 47 ]. CS activities were high and compatible with values obtained for other insects [ 59 ].…”

Section: Resultssupporting

confidence: 75%

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Mitochondrial Physiology in the Major Arbovirus Vector Aedes aegypti: Substrate Preferences and Sexual Differences Define Respiratory Capacity and Superoxide Production

2015

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Adult females of Aedes aegypti are facultative blood sucking insects and vectors of Dengue and yellow fever viruses. Insect dispersal plays a central role in disease transmission and the extremely high energy demand posed by flight is accomplished by a very efficient oxidative phosphorylation process, which take place within flight muscle mitochondria. These organelles play a central role in energy metabolism, interconnecting nutrient oxidation to ATP synthesis, but also represent an important site of cellular superoxide production. Given the importance of mitochondria to cell physiology, and the potential contributions of this organelle for A. aegypti biology and vectorial capacity, here, we conducted a systematic assessment of mitochondrial physiology in flight muscle of young adult A. aegypti fed exclusively with sugar. This was carried out by determining the activities of mitochondrial enzymes, the substrate preferences to sustain respiration, the mitochondrial bioenergetic efficiency and capacity, in both mitochondria-enriched preparations and mechanically permeabilized flight muscle in both sexes. We also determined the substrates preferences to promote mitochondrial superoxide generation and the main sites where it is produced within this organelle. We observed that respiration in A. aegypti mitochondria was essentially driven by complex I and glycerol 3 phosphate dehydrogenase substrates, which promoted distinct mitochondrial bioenergetic capacities, but with preserved efficiencies. Respiration mediated by proline oxidation in female mitochondria was strikingly higher than in males. Mitochondrial superoxide production was essentially mediated through proline and glycerol 3 phosphate oxidation, which took place at sites other than complex I. Finally, differences in mitochondrial superoxide production among sexes were only observed in male oxidizing glycerol 3 phosphate, exhibiting higher rates than in female. Together, these data represent a significant step towards the understanding of fundamental mitochondrial processes in A. aegypti, with potential implications for its physiology and vectorial capacity.

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Section: Resultssupporting

confidence: 75%

“…Previous investigations of mitochondrial physiology in A . aegypti flight muscle revealed that both respiration and ADP phosphorylation were induced by tricarboxylic acid cycle intermediates, in DDT-sensitive reactions [ 47 , 48 ]. Our group has shown that in females of A .…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial Physiology in the Major Arbovirus Vector Aedes aegypti: Substrate Preferences and Sexual Differences Define Respiratory Capacity and Superoxide Production

2015

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“…With serum albumin in the reaction medium P:O ratios were obtained approaching those found with mammalian preparations (Sacktor & Cochran, 1958;Cochran & King, 1960). Further, sarcosomes have been isolated from different insect species that exhibit respiratory control in the absence of ADP (Gonda et al 1957;Klingenberg & Bucher, -1959;Gregg et al 1960). Finally, it was shown that high P:O ratios could be obtained without addition of serum albumin and that pyruvate could be oxidized even more rapidly than oa-glycerophosphate (Gregg et al 1960).…”

Section: Discussionmentioning

confidence: 99%

“…Originally, no phosphorylation coupled to the oxidation of foodstuffs was found in sarcosomes from insect sources (Sacktor, 1953). But after Lewis & Slater (1953) had demonstrated oxidative phosphorylation in sarcosomes from the blowfly, their results were confirmed for sarcosomes from the locust (Rees, 1954), housefly (Sacktor, 1954), mosquito (Gonda et al 1957), wax-moth larva (Wojtczak & Wojtczak, 1959), cockroach (Cochran & King, 1960) and blowfly larva (Newburgh, Potter & Cheldelin, 1960). In most cases the addition of serum albumin to the reaction medium had a pronounced stimulatory effect on phosphate esterification.…”

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confidence: 91%

“…The lack of a measurable response of respiration of their isolated housefly sarcosomes to the addition of phosphate (Sacktor, 1954) or adenosine diphosphate (Sacktor & Cochran, 1958) led Chance & Sacktor (1958) and Estabrook & Sacktor (1958) to the proposals that the respiratory rate is controlled by the substrate concentration or by selective inhibition of certain oxidase systems. However, dependence of the rate of respiration of isolated insect sarcosomes on the concentration of adenosine diphosphate has now been firmly established (Gonda, Traub & Avi-Dor, 1957;Klingenberg & Bucher, 1959; Gregg et al 1960).…”

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confidence: 99%

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