The oxidation of (−)-epigallocatechin-3-gallate inhibits T-cell acute lymphoblastic leukemia cell line HPB-ALL <i>via</i> the regulation of Notch1 expression

Wang, Yuna; Wang, Jing; Yang, Haonan; Zhang, Bang-Lei; Zhang, Pan; Sun, Pengyan; Nin, Zhang; Wang, Ya; Sheng, Jun; Zi, Cheng-Ting

doi:10.1039/c9ra08459b

Cited by 9 publications

(7 citation statements)

References 37 publications

(42 reference statements)

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“…Recent studies have shown that EGCG has many excellent properties such as antitumor, antiradiation, and anti-oxidation capability [ 1 , 2 , 3 , 4 , 5 , 6 ]. However, EGCG is sensitive to neutral and alkaline solutions and becomes unstable as the oxygen concentration, temperature, and pH increases, resulting in impossible direct usage in these environments [ 7 , 8 , 9 , 10 ]. In addition, EGCG is unstable in the gastrointestinal environment, leading to low bioavailability [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Encapsulation of EGCG by Zein-Gum Arabic Complex Nanoparticles and In Vitro Simulated Digestion of Complex Nanoparticles

Jin

Liu

Tong

et al. 2022

Foods

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Epigallocatechin gallate (EGCG) has many excellent qualities such as its antitumor, antiradiation and anti-oxidation properties, but its application is limited because its oral bioavailability is low and stability is poor. In this paper, zein and gum arabic (GA) were used as wall materials to prepare Zein-GA complex nanoparticles for encapsulating and protecting the EGCG. The particle size of Zein-GA-EGCG complex nanoparticles ranged from 128.03–221.23 nm, and the EGCG encapsulation efficiency reached a maximum of 75.23% when the mass ratio of zein to GA was 1:1. The FTIR and XRD results illustrated that the components of the Zein-GA-EGCG complex nanoparticles interacted by electrostatic, hydrogen bonding, and hydrophobic interactions. The EGCG release rate of Zein-GA-EGCG nanoparticles (16.42%) was lower than that of Zein-EGCG (25.52%) during gastric digestion, and a large amount of EGCG was released during intestinal digestion, suggesting that the Zein-GA-EGCG nanoparticles could achieve the sustained release of EGCG during in vitro digestion. Hence, using Zein-GA complexes to encapsulate EGCG effectively increased the encapsulation efficiency of EGCG and realized the purpose of sustained release during simulated gastrointestinal digestion.

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Section: Introductionmentioning

confidence: 99%

Encapsulation of EGCG by Zein-Gum Arabic Complex Nanoparticles and In Vitro Simulated Digestion of Complex Nanoparticles

Jin

Liu

Tong

et al. 2022

Foods

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“…T8154; Sigma-Aldrich, St. Louis, Missouri, USA) staining was performed, and cell size determined using a JIMBIO FIL flow image counter (Jiangsu Zhuowei Biotechnology Co., Ltd., Changzhou, China). Chemical structure of D-EGCG.D-EGCG, dimeric (−)-epigallocatechin gallate [4].…”

Section: Analysis Of Cell Sizementioning

confidence: 99%

“…1. In previous research, D-EGCG has been found to exhibit anti-tumor activity [4], and the mechanism underlying the anti-tumor effects of D-EGCG warrants exploration.…”

Section: Introductionmentioning

confidence: 99%

Proliferation of MDA-MB-231 can be suppressed by dimeric-epigallocatechin gallate through competitive inhibition of amphiregulin-epidermal growth factor receptor signaling

Huang¹,

Zhang

Pang

et al. 2021

Anti-Cancer Drugs

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Triple-negative breast cancer (TNBC) is highly aggressive, with high rates of early relapse and very poor overall prognosis. Amphiregulin (AREG) is the most abundant epidermal growth factor receptor (EGFR) agonist in MDA-MB-231 TNBC cells, whose proliferation can be inhibited by (–)-epigallocatechin gallate (EGCG), a constituent of green tea that is prone to oxidative polymerization. The effect of dimeric-EGCG, a dimer of oxidized and polymerized EGCG, on MDA-MB-231 cell the proliferation warrants further exploration. In the present study, MTT, flow cytometry, migration scratch, transwell, western blotting, and surface plasmon resonance assays were used to evaluate the effect of dimeric-EGCG on MDA-MB-231 cells and explore the underlying mechanism. MDA-MB-231 cell proliferation and migration were significantly inhibited by dimeric-EGCG at concentrations as low as 10 μM. Levels of EGFR and p44/42 MAPK phosphorylation in MDA-MB-231 cells were significantly reduced by treatment with 10 μM dimeric-EGCG (P < 0.01). In addition, the levels of phosphorylation induced by exogenous AREG were also inhibited by dimeric-EGCG (P < 0.01); however, no significant effects of dimeric-EGCG were observed on epidermal growth factor or transforming growth factor-alpha signaling. Surface plasmon resonance analysis demonstrated that 10 μM dimeric-EGCG bound directly to the extracellular domain of EGFR, competitively inhibiting the binding of AREG to EGFR. These results suggest a novel mechanism underlying the inhibitory effect of dimeric-EGCG on MDA-MB-231 cells, with potential application in the development of drugs for the treatment of TNBC.

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“…Firstly, Ag-catalyzed 6-endo-dig oxocyclization of β-alkynyl The Notch signaling pathway plays a diverse role during the development and maintenance of normal tissues, suggesting that the Notch pathway could be a novel cancer therapeutic target. [16] Recently, the tetrahydroquinolines have shown interesting properties against a variety of human cancer cell-lines, [17] especially in the Notch-dependent cell line, U937 [18] and HPB-ALL, [19] which means that the derivatives of tetrahydroquinoline have potential treatment effect of disease related to Notch. Hence, the anticancer activity of our newly synthesized compounds was evaluated in U937 cells.…”

mentioning

confidence: 99%

Silver‐Catalyzed Tandem Reaction of β‐Alkynyl Ketones toward Spiro[isochromene‐1,4′‐quinoline] with Anticancer Activities

Yang

et al. 2023

Adv Synth Catal

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Spirocyclic frameworks could enhance the drug-like properties of planar bioactive molecules by increasing their three-dimensionality, making the development of synthetic methodology and bioactivity assays for spiro compounds highly attractive. This work reported the silver-catalyzed tandem cycloisomerization/Povarov reaction between β-alkynyl ketones and hexahydro-1,3,5-triazines, access to spiro[isochromene-1,4'-quinoline]. This synthetic protocol was characterized by remarkable efficiency, low catalyst-loading and high diastereoselectivity. Moreover, the spirocyclic quinolines exhibited good cytotoxicity in U937 cells by activating the Notch-signaling pathway exclusively.

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The oxidation of (−)-epigallocatechin-3-gallate inhibits T-cell acute lymphoblastic leukemia cell line HPB-ALL via the regulation of Notch1 expression

Abstract: T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive hematological malignancy, and commonly associated with activating mutations in the Notch1 pathway.

Cited by 9 publications

References 37 publications

Encapsulation of EGCG by Zein-Gum Arabic Complex Nanoparticles and In Vitro Simulated Digestion of Complex Nanoparticles

Encapsulation of EGCG by Zein-Gum Arabic Complex Nanoparticles and In Vitro Simulated Digestion of Complex Nanoparticles

Proliferation of MDA-MB-231 can be suppressed by dimeric-epigallocatechin gallate through competitive inhibition of amphiregulin-epidermal growth factor receptor signaling

Silver‐Catalyzed Tandem Reaction of β‐Alkynyl Ketones toward Spiro[isochromene‐1,4′‐quinoline] with Anticancer Activities

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