The oxidation of anthraquinone dye using HRP immobilized as a cross-linked enzyme aggregates

Abstract

Finding a sustainable and ecofriendly methods for recalcitrant synthetic dyes removal is a researchers` major challenge. A carrier-free technique for commercial HRP immobilization is a focus of the present study. The immobilized biocatalyst, HRP-CLEAs with 580 U g-1 of the activity was obtained under the following immobilization conditions: precipitation reagent 80% ammonium sulphate, cross-linking reagent 1% of glutaraldehyde and protein-fedder, bovine serum albumin (BSA) concentration 5 mg ml-1. The obtained HRP-CLEAs showed great affinity towards model anthraquinone dye, C. I. Acid Violet 109. 88.4% of the dye was oxidized under the reaction conditions: pH 4, dye concentration 40 mg l-1 , H 2 O 2 concentration 1 mM and 0.1 U of HRP-CLEAs. The possibility of the immobilized biocatalyst application in five and eight oxidation cycles of the dye and pyrogallol (retained activity ~ 80%), respectively, indicates that HRP-CLEAs is an efficient and environmentally friendly biocatalyst with great potential in aromatic compounds removal from wastewater. This paper is a continuation of our earlier research related to HRP from horseradish extract immobilization in the form of CLEAs and the application in the wastewater colored with a synthetic anthraquinone dye treatment.

Microfluidic technology for macro systems: Removal of textile dyes from wastewater in a microreactor

“…In this study, investigation of anthraquinone dye AV109 biodegradation by horseradish peroxidase in a microreactor is performed. Previous research studies have shown that the optimal pH value for degradation of AV109 by peroxidase is 4 [16,17].…”

Facile Fabrication of a Novel Copper Nanozyme for Efficient Dye Degradation

“…Horseradish peroxidase (HRP) can be applied for treating dye wastewater. − However, it is expensive and cannot work well under harsh conditions . In recent years, some artificial nanozymes, possessing a nanostructure and similar enzyme activity, have been successfully designed to overcome the disadvantages.…”