The oxidation of adrenaline and other amines

Blaschko, H.; Richter, Derek; Schlossmann, Hans

doi:10.1042/bj0312187

Cited by 388 publications

(72 citation statements)

References 7 publications

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“…Secondly, the response to epinine differed preparation of guinea-pig liver was used. Each from that to dopamine, although both amines are manometer flask contained 50 mg. of powder, oxidized by guinea-pig amine oxidase at a similar thoroughly washed with 0.067 M-sodium phosphate rate (Blaschko et al, 1937;Beyer, 1943;Randall, buffer of pH 7.4. At the zero moment, 0.2 ml.…”

Section: Methodsmentioning

confidence: 99%

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The Action of Dopamine on the Arterial Blood Pressure of the Guinea‐pig

Hornykiewicz

1958

British Journal of Pharmacology and Chemotherapy

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The depressor action of dopamine (8-3: 4-dihydroxyphenylethylamine) upon the arterial blood pressure of the guinea-pig has been studied. This effect begins without a latent period. It is often enhanced after the intravenous injection of iproniazid (Marsilid). The depressor response is sufficiently sensitive to serve as a method of bioassay of dopamine in microgram quantities. Observations on the depressor action of L-dopa have also been made. This effect is also enhanced by iproniazid; it begins after a latent period. Epinine (f8-3: 4-dihydroxyphenylethylmethylamine) caused a pressor response, followed by a fall of arterial blood pressure. No evidence was obtained in support of the suggestion that the two amines, which are oxidized at similar rates by amine oxidase, cause a fall of blood pressure after their conversion to an aldehyde by the action of amine oxidase.

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Section: Methodsmentioning

confidence: 99%

“…The fall of the blood pressure after administration of dopamine was ascribed by Holtz and Credner (1942) to the action of the aldehyde produced when dopamine is oxidized by the enzyme amine oxidase. It is known that dopamine is very rapidly oxidized by this enzyme (Blaschko, Richter, and Schlossmann, 1937).…”

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confidence: 99%

The Action of Dopamine on the Arterial Blood Pressure of the Guinea‐pig

Hornykiewicz

1958

British Journal of Pharmacology and Chemotherapy

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“…Monoamine oxidase is an intracellular enzyme, principally found in all tissues and predominantly bound to the inner mitochondrial membrane (14,15). Many methods for determination of monoamine oxidase activity have been developed.…”

Section: Discussionmentioning

confidence: 99%

Determination of Platelet Monoamine Oxidase by New Continuous Spectrophotometric Method

Ivanović

Majkić-Singh²

1988

Clinical Chemistry and Laboratory Medicine

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Summary:A simple, continuous Spectrophotometric method for the determination of tissue monoamine oxidase based on the oxidation of 2,2'-azino-di-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) using peroxidase has already been described (Ivanovic, I. & Majkic-Singh, N. (1986) Fresenius Z. Anal. Chem. 324, 307). In the present study the method is optimised for platelet monoamine oxidase assay and applied to healthy persons and schizophrenic patients. The obtained data were statistically analysed. The continuous ABTS method is sensitive, precise (CV below 6. The reference values for the activity of human platelet monoamine oxidase by the new continuous ABTS method are 25 to 42 U/g protein (x = 33.2 U/g protein, CV = 15.5%, n = 67). No differences were found between females and males, or between three age groups ranging from 21 to 52 years. The patients with chronic (n = 76) or acute (n = 17) schizophrenia had significantly lower monoamine oxidase activities compared with normal values (p < 0.005), which indicates that platelet monoamine oxidase can be a possible marker for schizophrenic diseases.

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“…Reduced glutathion was added to the homogenates to prevent autoxidation of adrenaline, as was described by Blaschko et al (2). For the determination of the inhibitory ED,,, on MAO, the concentration of the compounds in the reaction mixture was modified from 10-3 to 10-s Mole.…”

Section: Methodsmentioning

confidence: 99%