The Ovotransferrin-Derived Peptide IRW Attenuates Lipopolysaccharide-Induced Inflammatory Responses

Abstract

IRW (Ile-Arg-Trp), a bioactive peptide isolated from egg ovotransferrin, has been shown to exert anti-inflammatory effects. In this study, the effects of IRW on inflammatory cytokines and microbiota were explored in human umbilical vein endothelial cells (HUVECs) and a lipopolysaccharide (LPS)-induced rat model of inflammatory peritonitis. Rats were injected intraperitoneally with LPS to establish peritonitis. HUVECs were exposed to IRW for 12 h before introducing LPS. Notably, IRW exerted beneficial effects against LPS-induced peritonitis, specifically, by reducing the serum levels of tumour necrosis factor (TNF)-α and interleukin (IL)-6 and myeloperoxidase (MPO) activity (P<0.05). A faecal microbiota analysis revealed that IRW significantly increased the Shannon and decreased the Simpson indices (P<0.05). Furthermore, IRW treatment significantly inhibited the LPS-induced enhancement of TNF-α, IL-8, intercellular cell adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1) expression in HUVECs (P<0.05). In conclusion, IRW supplementation inhibited the inflammatory mediator synthesis and LPS-induced inflammatory responses and influenced the gut microbiota.

Egg Protein Transferrin-Derived Peptides IRW and IQW Regulate Citrobacter rodentium-Induced, Inflammation-Related Microbial and Metabolomic Profiles

“…A large number of experimental results indicate that colonic inflammation is accompanied by a decrease in colon length and other different degrees of morphological damage (Gearry et al, 2009; Gadaleta et al, 2011). Accordingly, IRW and IQW treatment appears to play a role in colonic inflammation (Jiao et al, 2019).…”

Enzymatic Hydrolysis of Ovotransferrin and the Functional Properties of Its Hydrolysates

“…Recently, Yu et al, (2019) exhibited potent ACE-inhibitory of ovotransferrin hydrolysates of 19 enzymes and one combination obtained from in silico hydrolysis. In addition, the peptides of ovotransferrin possess various immunomodulatory activities (Liu et al, 2017;Jiao et al, 2019), anti-inflammatory (Wang et al, 2017), and cytotoxic (Yi et al, 2017) activities.…”

Functional properties of ovotransferrin from chicken egg white and its derived peptides: a review

“…In an in vivo study, the Ile-Arg-Trp from ovotransferrin inhibited lipopolysaccharide (LPS)-induced peritonitis in rat models by inhibiting the synthesis of serum TNF and IL-8 and myeloperoxidase. Also, Ile-Arg-Trp treatment increased the Shannon index and decreased the Simpson index and suggested that modification of gut microbial diversity due to the potential ability of Ile-Arg-Trp to enhance the barrier properties of the intestine (Jiao et al, 2019) (Table 2).…”