The overexpression of insect endogenous small RNAs in transgenic rice inhibits growth and delays pupation of striped stem borer (<i>Chilo suppressalis</i>
)

Jiang, Shan; Wu, Hao; Liu, Haoju; Zheng, Jie; Lin, Yongjun; Chen, Hao

doi:10.1002/ps.4477

Cited by 33 publications

(32 citation statements)

References 40 publications

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“…At the same time, RNAi has been widely used to investigate gene function in insects and has been especially employed to silence vital genes in N. lugens and other insects through the suppression of gene expression by injecting dsRNA or single-stranded RNA (siRNA) (Belles, 2010 ; Wang et al, 2012 ; Kola et al, 2015 ; Liu et al, 2015 ; Joga et al, 2016 ). Sustained and effective RNAi expressing dsRNA in the host plant or transgene-mediated RNAi developed to decrease gene expression at the same time will be very useful for pest control (Baum et al, 2007 ; Mao et al, 2007 ; Swevers et al, 2013 ; Zhu, 2013 ; Jiang et al, 2016 ; Yu et al, 2016 ; Gillet et al, 2017 ). TRE has become an important target in pest management and control, and new and effective TRE inhibitor products with practical significance as pesticides are being developed (Tang et al, 2012 , 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Study on the Effect of Wing Bud Chitin Metabolism and Its Developmental Network Genes in the Brown Planthopper, Nilaparvata lugens, by Knockdown of TRE Gene

et al. 2017

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The brown planthopper, Nilaparvata lugens is one of the most serious pests of rice, and there is so far no effective way to manage this pest. However, RNA interference not only can be used to study gene function, but also provide potential opportunities for novel pest management. The development of wing plays a key role in insect physiological activities and mainly involves chitin. Hence, the regulating role of trehalase (TRE) genes on wing bud formation has been studied by RNAi. In this paper, the activity levels of TRE and the contents of the two sugars trehalose and glucose were negatively correlated indicating the potential role of TRE in the molting process. In addition, NlTRE1-1 and NlTRE2 were expressed at higher levels in wing bud tissue than in other tissues, and abnormal molting and wing deformity or curling were noted 48 h after the insect was injected with any double-stranded TRE (dsTRE), even though different TREs have compensatory functions. The expression levels of NlCHS1b, NlCht1, NlCht2, NlCht6, NlCht7, NlCht8, NlCht10, NlIDGF, and NlENGase decreased significantly 48 h after the insect was injected with a mixture of three kinds of dsTREs. Similarly, the TRE inhibitor validamycin can inhibit NlCHS1 and NlCht gene expression. However, the wing deformity was the result of the NlIDGF, NlENGase, NlAP, and NlTSH genes being inhibited when a single dsTRE was injected. These results demonstrate that silencing of TRE gene expression can lead to wing deformities due to the down-regulation of the AP and TSH genes involved in wing development and that the TRE inhibitor validamycin can co-regulate chitin metabolism and the expression of wing development-related genes in wing bud tissue. The results provide a new approach for the prevention and management of N. lugens.

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Section: Discussionmentioning

confidence: 99%

Study on the Effect of Wing Bud Chitin Metabolism and Its Developmental Network Genes in the Brown Planthopper, Nilaparvata lugens, by Knockdown of TRE Gene

et al. 2017

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“…Previously, a study comparing the effectiveness of in vitro synthesized long dsRNA and an artificial microRNA (miRNA) targeting chitin synthase gene A in the brown planthopper revealed that long dsRNA was more effective in gene silencing, resulting in more significant effects on fecundity and ovary development compared to miRNA (Li et al, 2017). Also, although several other studies using in planta expressed miRNA-based approaches showed positive results (Agrawal et al, 2015; Jiang et al, 2017; He et al, 2019), complete plant resistance to insect herbivory was not accomplished in most cases. With regard to molecule sequence, the range of insect species affected by a single dsRNA product depends on the gene target.…”

Section: Introductionmentioning

confidence: 91%

RNA Interference in the Tobacco Hornworm, Manduca sexta, Using Plastid-Encoded Long Double-Stranded RNA

Burke

Kaplanoglu

Kolotilin³

et al. 2019

Front. Plant Sci.

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RNA interference (RNAi) is a promising method for controlling pest insects by silencing the expression of vital insect genes to interfere with development and physiology; however, certain insect Orders are resistant to this process. In this study, we set out to test the ability of in planta-expressed dsRNA synthesized within the plastids to silence gene expression in an insect recalcitrant to RNAi, the lepidopteran species, Manduca sexta (tobacco hornworm). Using the Manduca vacuolar-type H+ ATPase subunit A (v-ATPaseA) gene as the target, we first evaluated RNAi efficiency of two dsRNA products of different lengths by directly feeding the in vitro-synthesized dsRNAs to M. sexta larvae. We found that a long dsRNA of 2222 bp was the most effective in inducing lethality and silencing the v-ATPaseA gene, when delivered orally in a water droplet. We further transformed the plastid genome of the M. sexta host plant, Nicotiana tabacum, to produce this long dsRNA in its plastids and performed bioassays with M. sexta larvae on the transplastomic plants. In the tested insects, the plastid-derived dsRNA had no effect on larval survival and no statistically significant effect on expression of the v-ATPaseA gene was observed. Comparison of the absolute quantities of the dsRNA present in transplastomic leaf tissue for v-ATPaseA and a control gene, GFP, of a shorter size, revealed a lower concentration for the long dsRNA product compared to the short control product. We suggest that stability and length of the dsRNA may have influenced the quantities produced in the plastids, resulting in inefficient RNAi in the tested insects. Our results imply that many factors dictate the effectiveness of in planta RNAi, including a likely trade-off effect as increasing the dsRNA product length may be countered by a reduction in the amount of dsRNA produced and accumulated in the plastids.

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“…Rice is an important crop, and a staple food for half of the world’s population 45 . Insect‐related losses in rice production are estimated to be 10% annually 46 .…”

Section: Introductionmentioning

confidence: 99%

Cloning and functional identification of a Chilo suppressalis‐inducible promoter of rice gene, OsHPL2

Wang

Han

et al. 2020

Pest Management Science

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BACKGROUNDPromoters play a key role in driving insect‐resistant genes during breeding of transgenic plants. In current transgenic procedures for breeding rice resistance to striped stem borer (Chilo suppressalis Walker, SSB), the constitutive promoter is used to drive the insect‐resistant gene. To reduce the burden of constitutive promoters on plant growth, isolation and identification of insect‐inducible promoters are particularly important. However, few promoters are induced specifically by insect feeding.RESULTSWe found rice hydroperoxide lyase gene (OsHPL2) (LOC_Os02g12680) was upregulated after feeding by SSB. We subsequently cloned the promoter of OsHPL2 and analysed its expression pattern using the β‐glucuronidase (GUS) reporter gene. Histochemical assays and quantitative analyses of GUS activity confirmed that P HPL2:GUS was activated by SSB, but did not respond to brown planthopper (Nilaparvata lugens Stål, BPH) infestation, mechanical wounding or phytohormone treatments. A series of 5′ truncated assays were conducted and three positive regulatory regions (−1452 to −1213, −903 to −624, and −376 to −176) induced by SSB infestation were identified. P2R123‐min 35S and P2TR2‐min 35S promoters linked with cry1C of transgenic plants showed the highest levels of Cry1C protein expression and SSB larval mortality.CONCLUSIONWe identified an SSB‐inducible promoter and three positive internal regions. Transgenic rice plants with the OsHPL2 promoter and its positive regions driving cry1C exhibited the expected larvicidal effect on SSB. Our study is the first report of an SSB‐inducible promoter that could be used as a potential resource for breeding insect‐resistant transgenic crops. © 2020 Society of Chemical Industry

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The overexpression of insect endogenous small RNAs in transgenic rice inhibits growth and delays pupation of striped stem borer (Chilo suppressalis )

Cited by 33 publications

References 40 publications

Study on the Effect of Wing Bud Chitin Metabolism and Its Developmental Network Genes in the Brown Planthopper, Nilaparvata lugens, by Knockdown of TRE Gene

Study on the Effect of Wing Bud Chitin Metabolism and Its Developmental Network Genes in the Brown Planthopper, Nilaparvata lugens, by Knockdown of TRE Gene

RNA Interference in the Tobacco Hornworm, Manduca sexta, Using Plastid-Encoded Long Double-Stranded RNA

Cloning and functional identification of a Chilo suppressalis‐inducible promoter of rice gene, OsHPL2

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