The ovarian response and embryo recovery rate in Boer goat does following different superovulation protocols, during the breeding season

Lehloenya, K.C.; Greyling, J.P.C.

doi:10.1016/j.smallrumres.2009.11.007

Cited by 22 publications

(21 citation statements)

References 29 publications

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“…The CL number observed in this study were lower than those reported in studies conducted in Angora (15.1), Saanen (29.3), Alpine (14.3) and Boer (21.3) goats in the non-breeding season (Tervit et al 1985;Menchaca et al 2007b;Lehloenya et al 2008). On the other hand, the CL number observed in this study were similar to those of (5.3 vs 4.0) previously reported for Angora goats by Tervit et al (1985) and Boer goats (Lehloenya and Greyling 2010). Lower CL proportion could be due to the breed, hormonal response of ovaries, climatic conditions and day light duration of the treatment period.…”

Section: Resultssupporting

confidence: 90%

Ovarian response and embryo yield of Angora and Kilis goats given the day 0 protocol for superovulation in the non-breeding season

Taşdemir¹,

Ağaoğlu

Kaymaz

et al. 2011

Trop Anim Health Prod

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The aim of this study was to compare ovarian response and embryo yield of Day 0 protocol in Angora goats (AG) and indigenous Kilis goats (KG) in the non-breeding season. A total of 16 Angora goats (AG group) and 11 Kilis goats (KG group) were used in this study. In the synchronization process, after controlled internal drug release withdrawal, when estrus signs were observed, natural mating was performed. Ovarian response was determined by synchronized laparotomy 6 days after natural mating, and number of corpora lutea (CL) was recorded. Embryos were collected and morphologically evaluated by stereomicroscope. Synchronization rates did not differ between AG (88%, 14/16) and KG group (91%, 10/11). In AG and KG groups, the proportion of CL on the right (44% and 53%, respectively) and left (56% and 47%, respectively) ovaries were similar. The CL number per animal did not differ significantly between the two breeds and was determined as 4.4 ± 0.90 in AG group and 6.4 ± 1.44 in KG group. Transferable embryo yields were significantly higher in AG group (31/42, 74%) compared to KG group (16/46, 35%) in the non-breeding season (P < 0.01). In conclusion, it is suggested that the day 0 protocol can be used for goat superovulation in the non-breeding season; however, transferable embryo yields are affected by the breed.

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Section: Resultssupporting

confidence: 90%

Ovarian response and embryo yield of Angora and Kilis goats given the day 0 protocol for superovulation in the non-breeding season

Taşdemir¹,

Ağaoğlu

Kaymaz

et al. 2011

Trop Anim Health Prod

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“…The day 0 group had a significantly lower number of CLs, and this finding corresponds to the results obtained by a similar protocol in the study conducted by MENCHACA et al (2007) and LEHLOENYA and GREYLING (2010).…”

Section: Discussionsupporting

confidence: 88%

Efficiency analysis of standard and day 0 superovulatory protocols in Boer breed goats

et al. 2017

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The aim of this research was to investigate the ovarian influence on the success of two superovulatory protocols in goats. Fifteen goats were used twice in the study in a crossover design: once within the control group where standard protocol was applied, and the other time in a modified, day 0 group, where no dominant follicle was present and the follicular cohort was homogenous ("Day 0 protocol"). All the animals were synchronized with vaginal pessaries and superovulated with 6 decreasing doses of pFSH. Although the day 0 protocol significantly decreased the presence of the dominant follicle before the start of the superovulation treatment; it did not improve the superovulatory success. Fifty-four percent of goats in the control group had a dominant follicle which exerted a negative influence on the number of transferable embryos. The other 46% of goats from the same group had a very good superovulatory response, which improved the overall response of the control group in comparison with the day 0 group. Another reason for the lower superovulatory success of the day 0 protocol was the smaller number of second and third category follicles (3.5 -5.5 mm in size) at the onset of the superovulation protocol, that positively correlated with the number of the transferable embryos (r=0.67; P<0.05). In Boer goats, for the success of superovulatory treatment, the number of follicles of a certain size before the start of the superovulatory treatment has an effect as important as the effect of dominance. The application of the FSH hormone should start at the time when a suitable number of follicles belonging to the second category are present on the ovaries when performing the day 0 protocol.

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“…In this study, all does used as donor are within their peak of reproduction owing to the acceptable mean number of transferrable embryos recovered following flushing, though lower compared to superovulation of dairy goats, Murciano-Granadina (14.7±2.5) and Jakhrana goats (11.8±2.9) (Gonzales-Bulnes et al, 2003;Goel et al, 2005). Moreover, the FSH dosage used in the induction of superovulation in Boer was 200 mg/doe (Lehloenya and Greyling, 2010), almost ten folds higher in this study which similarly had comparable results. These observations could be ascribed to the difference on the breed used, Boer being bigger and heavier by almost 5 folds to non-descript Philippines goats.…”

Section: Discussionmentioning

confidence: 88%

“…In contrast, past studies with Boer goats involves the use of the long synchronization program (17 days) during and outside the natural breeding season without PGF2α treatment getting a mean ovulation rate of 16.5 ±0.8. But when the oestrous cycle was synchronized with controlled internal drug release dispenser (CIDR, Pharmacia & Upjohn, Auckland, New Zealand) for 7 days plus PGF 2 α (0.05 mg/doe) at the time of CIDR insertion, only mean ovulation rate of 4.0 ± 0.7 was obtained (Lehloenya and Greyling, 2010). In this study, the super-ovulation treatment started on the 16 th day of the cycle without intravaginal progestagen treatment and PGF 2 α was administered at a later hour (48 and 60) after the 1 st injection of gonadotropins resulting in a mean ovulation rate of 13.07 in 14 does, while 3 did not respond to treatment.…”

Section: Discussionmentioning

confidence: 99%

Live birth after transfer of vitrified embryos from superovulated goats

2016

Res. Opin. Anim. Vet. Sci.

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In this study, the viability of vitrified goat embryos recovered from superovulated nondescript Philippine goats were evaluated post-warming by transferring to surrogate does. Of 17 does treated for superovulation, 3 did not respond and 14 responded with a 13.07 mean ovulation rate. An average of 9.7 embryos per doe was collected at different developmental stages. Using a container-less minimum drop size (MDS) method of vitrification, 50 and 64 embryos were exposed to vitrification solution 1 (VS1) containing 10% ethylene glycol in basic medium (BM) consisting of Hepes buffered TCM-199 medium + 20% estrus-doe serum solution for 3 min and 10 min, respectively. Immediately thereafter, the embryos were transferred to 40% EG + 1 M sucrose in BM for 45 Sec before recovering and dropping directly into LN 2. After 3 months of storage, the embryos were warmed in 2 ml of 0.3 M sucrose in BM for 5 min and washed twice before transferring into culture medium. Morphologically normal embryos were then transferred surgically to 6 recipients. Seven kids were born after a normal gestation period with birthweights ranging from 0.78-2.5 kg. The results highlight the usefulness of the MDS method of vitrification using an ethylene glycol based solution in the cryopreservation of goat embryos. That, continuous research effort should be made for optimizing cryopreservation protocols for conservation of animal genetic resources. Keywords: Embryo transfer; live birth; superovulation; vitrification To cite this article: Ocampo MB, JQ Silvestre, VD Viernes and LC Ocampo, 2016. Live birth after transfer of vitrified embryos from superovulated goats. Res. Opin. Anim. Vet. Sci., 6(2): 47-52.

show abstract

The ovarian response and embryo recovery rate in Boer goat does following different superovulation protocols, during the breeding season

Cited by 22 publications

References 29 publications

Ovarian response and embryo yield of Angora and Kilis goats given the day 0 protocol for superovulation in the non-breeding season

Ovarian response and embryo yield of Angora and Kilis goats given the day 0 protocol for superovulation in the non-breeding season

Efficiency analysis of standard and day 0 superovulatory protocols in Boer breed goats

Live birth after transfer of vitrified embryos from superovulated goats

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