The ovarian and hypogastric innervation of the guinea-pig uterus

Isaac, P.F.; Pennefather, Jocelyn N.; Silva, Douglas G.

doi:10.1016/0014-2999(69)90117-4

Cited by 14 publications

(9 citation statements)

References 9 publications

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“…It has been proposed that the pregnancy-induced loss of fluorescence is restricted to the intrauterine axons because these also arise from 'short' adrenergic neurones lying in the pelvic region (Sjöberg, 1968 ;Owman, Sjöberg & Sjöstrand, 1974). Such an explanation is, however, in conflict with evidence presented by other workers which suggests that the adrenergic innervation of the guinea-pig uterus arises primarily from 'long' adrenergic neurones and enters the uterus with the ovarian artery (Isaac, Pennefather & Silva, 1969; Kulkarni, Wakade & Kirpekar, 1976). In the present study we have re-examined the origins of the intrauterine adrenergic nerves and made some observations on the probable mechanism of loss of fluorescence during pregnancy.…”

Section: Introductioncontrasting

confidence: 74%

“…He concluded that these axons arose from 'short' adrenergic neurones which had their cell bodies in the pelvic plexus. Other workers have considered that the adrenergic supply to the guinea-pig uterus is by 'long' neurones which travel with the ovarian artery (Isaac et al, 1969;Kulkarni et al, 1976). The present results confirm the report of Langley & Anderson (1895) that the guinea-pig uterus receives sympathetic axons from the ovarian and pelvic ends and offer a way of reconciling the conflicting views held by later workers.…”

Section: Discussionmentioning

confidence: 99%

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Observations on the loss of catecholamine fluorescence from intrauterine adrenergic nerves during pregnancy in the guinea-pig

Bell¹,

Malcolm²

1978

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Summary. During unilateral pregnancy in the guinea-pig there is loss of formaldehyde\x=req-\ induced fluorescence from the adrenergic nerves supplying the uterus and its vasculature. This loss occurs initially near the site of implantation at about Day 20 of gestation and spreads progressively. Implantation of wax pellets containing progesterone into the uterine lumen or the gastrocnemius muscle of virgin guinea-pigs for 7 days produced loss of fluorescence from all local adrenergic nerves. No diminution of fluorescence was seen when pellets containing oestradiol were substituted. Chronic denervation studies showed that the adrenergic axons supplying the uterus and its arteries originated from both the ovarian artery and the pelvic region. Our results suggest that loss of adrenergic fluorescence within the uterus during pregnancy is due to an effect of placental progesterone which is localized to the uterus because the high concentration of progesterone necessary to cause fluorescence loss is not attained in the systemic circulation.

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Section: Introductioncontrasting

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Observations on the loss of catecholamine fluorescence from intrauterine adrenergic nerves during pregnancy in the guinea-pig

Bell¹,

Malcolm²

1978

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“…The importance of short adrenergic neurones is unknown but the ability of steroid hormones to influence their transmitter metabolism may enable them to respond specifically to physiological conditions such as pregnancy (Sjoberg, 1967;Rosengren & Sjoberg, 1968;Falck, Owman, Rosengren & Sjoberg, 1969;Owman, Sjoberg & Sjostrand, 1974;Thorbert, Batra, Owman, Rosengren & Sjoberg, 1976;Thorbert, 1978). Neuronal or exogenous noradrenaline acting via alpha-and beta-adrenoreceptors has been reported to contract (Zuspan, Cibils & Pose, 1962;Miller & Marshall, 1965;Isaac & Pennefather, 1969;Rexroad & Barb, 1978) and relax (Miller & Marshall, 1965;Bengtsson, 1978;Elmer, Aim & Thorbert, 1980) uterine smooth muscle depending on animal species and hormonal state. Adrenergic stimulation also indirectly influences myometrial contractility by increasing sensitivity to oxytocin (Russe & Marshall, 1970).…”

Section: Introductionmentioning

confidence: 99%

Regional changes in catecholamine content of the pregnant uterus

Arkinstall¹,

Jones²

1985

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Summary. High-pressure liquid chromatography with electrochemical detection was used to identify and measure catecholamines in rat, rabbit, sheep, guinea-pig and human uteri and follow changes with pregnancy. Noradrenaline was consistently the major catecholamine and pregnancy caused a regionally specific fall in its concentration which, in rat, rabbit and guinea-pig, was associated with a decline in total content. Adrenaline was undetectable (<10 pmol/g myometrium) in all species and at all gestational ages studied. Dopamine and its metabolite 3,4-dihydroxyphenylacetic acid (DOPAC) were detected at high concentrations in guinea-pig and particularly sheep uterus. In guinea-pig uterus the dopamine/DOPAC ratio fell dramatically with pregnancy, suggesting that increased quantities of dopamine were released and catabolized. The dopamine/noradrenaline ratios suggested that dopamine is stored with noradrenaline in adrenergic neurones in guinea-pig myometrium and within an additional neuronal or cellular store(s) in sheep uterus.

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“…The present study of the effects of ovarian steroids upon the response of the guinea-pig uterus to adrenergic influences differs from earlier studies (Hermansen, 1960;Pennefather & Isaac, 1967;Isaac et al, 1969;Russe & Marshall, 1970;Szurszewski & Builbring, 1973;Elmer, Alm & Thorbert, 1980) in that the two myometrial layers have been studied separately. This major methodological modification has allowed us to establish that the administration of progesterone to oestrogen-primed animals leads, in both ovariectomized and intact animals, to a clearcut, reproducible reversal of the effects of phenylephrine, in the longitudinal, but not in the circular myometrial layer.…”

Section: Discussionmentioning

confidence: 72%

“…These effects have been demonstrated using uterine horns from the guinea-pig (Hermansen, 1960;Pennefather & Isaac, 1967;Isaac, Pennefather & Silva, 1969;Russe & Marshall, 1970), the cat (Dale, 1906;Cushny, 1906;Gustavson & van Dyke, 1931;Tsai & Fleming, 1964), the rabbit (Miller & Marshall, 1965), and the rat (Diamond & Brody, 1966;AbdelAziz & Bakry, 1973). Electrophysiological studies with guinea-pig myometrium by Szurszewski & Billbring (1973), have done much to establish the ionic basis of the excitatory and inhibitory actions of catecholamines mediated via actions at uterine a-and 13-adrenoceptors.…”

Section: Introductionmentioning

confidence: 98%

Effects of ovarian steroids upon responses mediated by adrenoceptors in separated layers of the myometrium and in the costo‐uterine muscle of the guinea‐pig

Hartley

Pennefather

Story

1983

British J Pharmacology

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1This study describes the effects of ovarian steroid hormones upon the responses to adrenoceptor agonists of isolated myometrium, separated into its longitudinal and circular layers, and of costo-uterine muscle from guinea-pigs. The preparations were field-stimulated at 100s intervals, and the adrenoceptor agonists phenylephrine and isoprenaline produced enhancement or inhibition of the evoked contractions. 2 Isoprenaline produced propranolol-sensitive inhibitory effects in longitudinal and circular myometrium and costo-uterine muscle preparations from animals from all experimental groups: i.e. from nonsteroid-treated animals (ovariectomized and intact); intact animals treated with either oestrogen or progesterone alone; ovariectomized animals treated with oestrogen; ovariectomized and intact animals treated with progesterone following oestrogen priming; and from animals 1-4 days post-partum. Longitudinal myometrial preparations from progesterone-treated oestrogenprimed and from post-partum animals were most sensitive to this agonist. 3 Phenylephrine produced phentolamine-sensitive excitatory effects in circular myometrial and costo-uterine muscle preparations from animals from all the experimental groups. In contrast, propranolol-sensitive inhibitory responses to phenylephrine occurred in longitudinal myometrial preparations taken from animals treated with progesterone following oestrogen priming, and from post-partum animals. Longitudinal myometrium from animals from the remaining experimental groups exhibited phentolamine-sensitive excitatory responses to phenylephrine. 4 The basis for the selective effect upon the longitudinal myometrium of exposure to progesterone following a period of oestrogen priming, is discussed. The results described are consistent with the possibility that in the longitudinal layer of guinea-pig uterus exposed to progesterone following oestrogen priming there is an increase in the proportion of P-adrenoceptors in this layer. This increase may reduce the likelihood of contractions arising via direct stimulation of oa-adrenoceptors in this layer in response to sympathetic activation during pregnancy.

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The ovarian and hypogastric innervation of the guinea-pig uterus

Cited by 14 publications

References 9 publications

Observations on the loss of catecholamine fluorescence from intrauterine adrenergic nerves during pregnancy in the guinea-pig

Observations on the loss of catecholamine fluorescence from intrauterine adrenergic nerves during pregnancy in the guinea-pig

Regional changes in catecholamine content of the pregnant uterus

Effects of ovarian steroids upon responses mediated by adrenoceptors in separated layers of the myometrium and in the costo‐uterine muscle of the guinea‐pig

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