The outer membrane vesicles: Secretion system type zero

Guerrero-Mandujano, Andrea; Hernández-Cortez, Cecilia; Ibarra, J. Antonio; Castro-Escarpulli, Graciela

doi:10.1111/tra.12488

Cited by 141 publications

(144 citation statements)

References 78 publications

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“…OMV are multifunctional structures that bleb from the outer membrane of bacteria and typically include proteins, phospholipids, nucleic acids, LPS, QS signals, ions, and metabolites. These extracellular structures are considered as a type zero secretion system (T0SS), an additional and independent traffic system [61]. Six classical types of protein secretion systems have been identified in Pseudomonas , termed T1SS to T6SS, and they are conserved in Gram-negative bacteria.…”

Section: Qs-regulated Factors That Impact P Aeruginosa Communities Amentioning

confidence: 99%

Bacteria-Host Crosstalk: Sensing of the Quorum in the Context of Pseudomonas aeruginosa Infections

Turkina

Vikström

2018

J Innate Immun

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Cell-to-cell signaling via small molecules is an essential process to coordinate behavior in single species within a community, and also across kingdoms. In this review, we discuss the quorum sensing (QS) systems used by the opportunistic pathogen Pseudomonas aeruginosa to sense bacterial population density and fitness, and regulate virulence, biofilm development, metabolite acquisition, and mammalian host defense. We also focus on the role of N-acylhomoserine lactone-dependent QS signaling in the modulation of innate immune responses connected together via calcium signaling, homeostasis, mitochondrial and cytoskeletal dynamics, and governing transcriptional and proteomic responses of host cells. A future perspective emphasizes the need for multidisciplinary efforts to bring current knowledge of QS into a more detailed understanding of the communication between bacteria and host, as well as into strategies to prevent and treat P. aeruginosa infections and reduce the rate of antibiotic resistance.

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Section: Qs-regulated Factors That Impact P Aeruginosa Communities Amentioning

confidence: 99%

Bacteria-Host Crosstalk: Sensing of the Quorum in the Context of Pseudomonas aeruginosa Infections

Turkina

Vikström

2018

J Innate Immun

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“…lack other systems that typically function in this capacity for intracellular Gram‐negative pathogens (Larsson et al ., ). The release of membrane‐derived vesicles provides an alternative route for the delivery of bacterial virulence factors into host cells (Guerrero‐Mandujano et al ., ). Previous work from our group and Pierson et al .…”

Section: Introductionmentioning

confidence: 97%

Amino acid deprivation and central carbon metabolism regulate the production of outer membrane vesicles and tubes by Francisella

Sampath

McCaig

Thanassi

2018

Molecular Microbiology

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Francisella tularensis is a highly virulent Gram-negative bacterial pathogen that causes the zoonotic disease tularemia. F. novicida, a model tularemia strain, produces spherical outer membrane vesicles (OMV), as well as novel tubular vesicles and extensions of the cell surface. These OMV and tubes (OMV/T) are produced in a regulated manner and contain known virulence factors. Mechanisms by which bacterial vesicles are produced and regulated are not well understood. We performed a genetic screen in F. novicida to decipher the molecular basis for regulated OMV/T formation, and identified both hypo- and hyper-vesiculating mutants. Mutations in fumA and tktA, involved in central carbon metabolism, and in FTN_0908 and FTN_1037, of unknown function, resulted in severe defects in OMV/T production. Cysteine deprivation was identified as the signal that triggers OMV/T formation in F. novicida during growth in rich medium. We also found that fully virulent F. tularensis produces OMV/T in a similarly regulated manner. Further analysis revealed that OMV/T production is responsive to deprivation of essential amino acids in addition to cysteine, and that the hypo-vesiculating mutants are defective in responding to this signal. Thus, amino acid starvation, such as encountered by Francisella during host cell invasion, regulates the production of membrane-derived structures.

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“…Whereas the type III, type IV, and type VI secretion systems imply direct contact between bacteria and their host to allow the release of molecules into the host cytosol, the release of OMVs provides a way to transfer bacterial cargo without being in close contact with the host and thus OMVs can act as long distance carriers (Jan, 2017; Tashiro et al, 2017). In this context, the classification of OMVs as a type 0 secretion system has been proposed recently (Guerrero-Mandujano et al, 2017). OMV delivery to their target occurs by different means, such as membrane fusion with the host or a variety of endocytosis mechanisms (O’Donoghue and Krachler, 2016).…”

Section: Introductionmentioning

confidence: 99%

The RNA Complement of Outer Membrane Vesicles From Salmonella enterica Serovar Typhimurium Under Distinct Culture Conditions

et al. 2018

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Bacterial outer membrane vesicles (OMVs), as well as OMV-associated small RNAs, have been demonstrated to play a role in host–pathogen interactions. The presence of larger RNA transcripts in OMVs has been less studied and their potential role in host–pathogen interactions remains largely unknown. Here we analyze RNA from OMVs secreted by Salmonella enterica serovar Typhimurium (S. Typhimurium) cultured under different conditions, which mimic host–pathogen interactions. S. Typhimurium was grown to exponential and stationary growth phases in minimal growth control medium (phosphate-carbon-nitrogen, PCN), as well as in acidic and phosphate-depleted PCN, comparable to the macrophage environment and inducing therefore the expression of Salmonella pathogenicity island 2 (SPI-2) genes. Moreover, Salmonella pathogenicity island 1 (SPI-1), which is required for virulence during the intestinal phase of infection, was induced by culturing S. Typhimurium to the stationary phase in Lysogeny Broth (LB). For each condition, we identified OMV-associated RNAs that are enriched in the extracellular environment relative to the intracellular space. All RNA classes could be observed, but a vast majority of rRNA was exported in all conditions in variable proportions with a notable decrease in LB SPI-1 inducing media. Several mRNAs and ncRNAs were specifically enriched in/on OMVs dependent on the growth conditions. Important to note is that some RNAs showed identical read coverage profiles intracellularly and extracellularly, whereas distinct coverage patterns were observed for other transcripts, suggesting a specific processing or degradation. Moreover, PCR experiments confirmed that distinct RNAs were present in or on OMVs as full-length transcripts (IsrB-1/2; IsrA; ffs; SsrS; CsrC; pSLT035; 10Sa; rnpB; STM0277; sseB; STM0972; STM2606), whereas others seemed to be rather present in a processed or degraded form. Finally, we show by a digestion protection assay that OMVs are able to prevent enzymatic degradation of given full-length transcripts (SsrS, CsrC, 10Sa, and rnpB). In summary, we show that OMV-associated RNA is clearly different in distinct culture conditions and that at least a fraction of the extracellular RNA is associated as a full-length transcripts with OMVs, indicating that some RNAs are protected by OMVs and thereby leaving open the possibility that those might be functionally active.

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The outer membrane vesicles: Secretion system type zero

Cited by 141 publications

References 78 publications

Bacteria-Host Crosstalk: Sensing of the Quorum in the Context of <b><i>Pseudomonas aeruginosa</i></b> Infections

Bacteria-Host Crosstalk: Sensing of the Quorum in the Context of <b><i>Pseudomonas aeruginosa</i></b> Infections

Amino acid deprivation and central carbon metabolism regulate the production of outer membrane vesicles and tubes by Francisella

The RNA Complement of Outer Membrane Vesicles From Salmonella enterica Serovar Typhimurium Under Distinct Culture Conditions

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