The osteoinductive properties of mesoporous silicate coated with osteostatin in a rabbit femur cavity defect model

Parathyroid hormone-related protein (107-111) improves the bone regeneration potential of gelatin–glutaraldehyde biopolymer-coated hydroxyapatite

“…Recently, we showed the osteoinductive actions of locally delivered PTHrP (107-111) from SBA15-based ceramics as implanted carriers into a rabbit bone defect [27,29]. This type of Si-enriched ceramic has a narrow mesopore size distribution (in the nanometre range) but a large surface area that governs the interaction with the host bone tissue.…”

“…This type of Si-enriched ceramic has a narrow mesopore size distribution (in the nanometre range) but a large surface area that governs the interaction with the host bone tissue. However, these materials were non-degradable and induced the formation of a thick fibrous cup around the implant [3,27,29]. This prompted us to evaluate whether loading PTHrP (107-111) into HA Glu scaffolds would provide a more appropriate biomaterial as an implant for improving new bone formation.…”

“…Consistent with previous data using other types of non-degradable ceramics as carriers of this pentapeptide [26], we observed here that, in vitro, PTHrP (107-111) was released very rapidly (within 2 days) from HA Glu scaffolds into the surrounding medium. Using the former materials, even small amounts of this peptide (in the nanomolar range or lower concentrations) remaining in the non-degradable ceramic seemed to elicit osteogenic actions [26,27,29]. The HA Glu scaffolds have been shown to be stable for about 2 weeks, but progressive degradation occurs thereafter [11].…”

“…In addition, the C-terminal 107-111 domain (also known as osteostatin) of PTHrP also exhibits osteogenic features in vitro, and stimulates bone accrual in vivo [17][18][19][20][21][22][23][24][25]. Of note, we recently showed that loading non-degradable Si-based ceramics with the latter pentapeptide gives them osteogenic and angiogenic features both in vitro and in vivo as implants in a cavitary defect in the rabbit femur [26][27][28][29]. These effects of osteostatin were thought to be due to its release into the local environment, because this type of ceramic elicits the formation of a fibrous capsule that hampers close contact between the implant and the surrounding tissue [27,29].…”

“…Of note, we recently showed that loading non-degradable Si-based ceramics with the latter pentapeptide gives them osteogenic and angiogenic features both in vitro and in vivo as implants in a cavitary defect in the rabbit femur [26][27][28][29]. These effects of osteostatin were thought to be due to its release into the local environment, because this type of ceramic elicits the formation of a fibrous capsule that hampers close contact between the implant and the surrounding tissue [27,29]. In any event, these recent observations point to PTHrP (107-111) as an interesting peptide in a bone tissue engineering scenario.…”