The OsSec18 complex interacts with P0(P1-P2)2 to regulate vacuolar morphology in rice endosperm cell

Sun, Yunfang; Ning, Tingting; Liu, Zhenwei; Pang, Jianlei; Jiang, Daiming; Guo, Zhang; Song, Gaoyuan; Yang, Daichang

doi:10.1186/s12870-014-0324-1

Cited by 7 publications

(6 citation statements)

References 25 publications

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“…Those genes deserved further study to unveil their possible role in TGW and their application in breeding programs. Although the relationship between these genes with grain development in wheat has not been reported, several homologous genes have been shown to participate in the regulation of TGW in rice, such as OsSec18 , OsBADH1 , and OsLHT1 , indicating that these 134 putative candidate genes could be involved in TGW regulation in wheat ( Tang et al, 2014 ; Sun et al, 2015 ; Guo et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Major Genomic Regions for Wheat Grain Weight as Revealed by QTL Linkage Mapping and Meta-Analysis

Miao

Jing

et al. 2022

Front. Plant Sci.

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Grain weight is a key determinant for grain yield potential in wheat, which is highly governed by a type of quantitative genetic basis. The identification of major quantitative trait locus (QTL) and functional genes are urgently required for molecular improvements in wheat grain yield. In this study, major genomic regions and putative candidate genes for thousand grain weight (TGW) were revealed by integrative approaches with QTL linkage mapping, meta-analysis and transcriptome evaluation. Forty-five TGW QTLs were detected using a set of recombinant inbred lines, explaining 1.76-12.87% of the phenotypic variation. Of these, ten stable QTLs were identified across more than four environments. Meta-QTL (MQTL) analysis were performed on 394 initial TGW QTLs available from previous studies and the present study, where 274 loci were finally refined into 67 MQTLs. The average confidence interval of these MQTLs was 3.73-fold less than that of initial QTLs. A total of 134 putative candidate genes were mined within MQTL regions by combined analysis of transcriptomic and omics data. Some key putative candidate genes similar to those reported early for grain development and grain weight formation were further discussed. This finding will provide a better understanding of the genetic determinants of TGW and will be useful for marker-assisted selection of high yield in wheat breeding.

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Section: Discussionmentioning

confidence: 99%

Major Genomic Regions for Wheat Grain Weight as Revealed by QTL Linkage Mapping and Meta-Analysis

Miao

Jing

et al. 2022

Front. Plant Sci.

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“…OsSec18 interacts with Os60sP0/OsP0A and together with other proteins constitutes a 290-kDa complex in rice endosperm cells. However, the function of OsP0A in the complex remains unclear (Sun et al, 2015). As P0 proteins are highly conserved, and OsP0s localizes both in cytoplasm and nucleus, we speculate that OsP0s probably also have evolved extraribosomal functions as its homologs did.…”

Section: The Evolution Of P0 Family Genes In Ricementioning

confidence: 89%

“…The human HsP0 directly bind to the RRM1 and RRM2 domains of NONO, therefore enhancing non-homologous end joining-mediated DSB repair (Wang et al, 2022). OsSec18 is a conserved ATPase required for vesicle membrane fusion (Sun et al, 2015). OsSec18 interacts with Os60sP0/OsP0A and together with other proteins constitutes a 290-kDa complex in rice endosperm cells.…”

Section: The Evolution Of P0 Family Genes In Ricementioning

confidence: 99%

The ribosomal protein P0A is required for embryo development in rice

Chang

Wang

Pan

et al. 2022

Preprint

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The P-stalk is a conserved and vital structural element of ribosome. The eukaryotic P-stalk exists as a P0-(P1-P2)2 pentameric complex, in which P0 function as a base structure for incorporating the stalk onto 60S pre-ribosome. There is only a single copy of P0 gene in yeast and most animal species. Prior studies have suggested that P0 genes are indispensable for survival in yeast and animals. In contrast, each plant species has at least two copies of P0 genes, however, the functions of P0 genes in plants remain elusive. In the present study, we show that rice has three P0 genes predicted to encode highly conserved proteins OsP0A, OsP0B and OsP0C. All of these P0 proteins were localized both in cytoplasm and nucleus, and conversely interacted with OsP1. Intriguingly, the transcripts of OsP0A presented more than 90% of the total P0 transcripts. Moreover, knockout of OsP0A led to embryo lethality, while single or double knockout of OsP0B and OsP0C did not show any comparable phenotypes in rice. The genomic DNA of OsP0A could well complement the phenotypes of osp0a mutant. Finally, sequences and syntenic analyses revealed that OsP0C evolved from OsP0A, and that duplication of genomic fragment harboring OsP0C further gave birth of OsP0B, and both of these duplication events happened prior to the differentiation of Xian and Geng subspecies in rice ancestor. Together, our data indicate that OsP0A functions as the predominant P0 gene, playing an essential role in embryo development in rice.

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“…The human HsP0 directly bind to the RRM1 and RRM2 domains of NONO, therefore enhancing non-homologous end joining-mediated DSB repair [ 47 ]. OsSec18 is a conserved ATPase required for vesicle membrane fusion [ 48 ]. OsSec18 interacts with Os60sP0/OsP0A and together with other proteins constitutes a 290-kDa complex in rice endosperm cells.…”

Section: Discussionmentioning

confidence: 99%

The ribosomal protein P0A is required for embryo development in rice

Chang,

Wang,

Pan

et al. 2023

BMC Plant Biol

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Background The P-stalk is a conserved and vital structural element of ribosome. The eukaryotic P-stalk exists as a P0-(P1-P2)2 pentameric complex, in which P0 function as a base structure for incorporating the stalk onto 60S pre-ribosome. Prior studies have suggested that P0 genes are indispensable for survival in yeast and animals. However, the functions of P0 genes in plants remain elusive. Results In the present study, we show that rice has three P0 genes predicted to encode highly conserved proteins OsP0A, OsP0B and OsP0C. All of these P0 proteins were localized both in cytoplasm and nucleus, and all interacted with OsP1. Intriguingly, the transcripts of OsP0A presented more than 90% of the total P0 transcripts. Moreover, knockout of OsP0A led to embryo lethality, while single or double knockout of OsP0B and OsP0C did not show any visible defects in rice. The genomic DNA of OsP0A could well complement the lethal phenotypes of osp0a mutant. Finally, sequence and syntenic analyses revealed that OsP0C evolved from OsP0A, and that duplication of genomic fragment harboring OsP0C further gave birth to OsP0B, and both of these duplication events might happen prior to the differentiation of indica and japonica subspecies in rice ancestor. Conclusion These data suggested that OsP0A functions as the predominant P0 gene, playing an essential role in embryo development in rice. Our findings highlighted the importance of P0 genes in plant development.

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The OsSec18 complex interacts with P0(P1-P2)2 to regulate vacuolar morphology in rice endosperm cell

Cited by 7 publications

References 25 publications

Major Genomic Regions for Wheat Grain Weight as Revealed by QTL Linkage Mapping and Meta-Analysis

Major Genomic Regions for Wheat Grain Weight as Revealed by QTL Linkage Mapping and Meta-Analysis

The ribosomal protein P0A is required for embryo development in rice

The ribosomal protein P0A is required for embryo development in rice

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