The OsNAC23-Tre6P-SnRK1a feed-forward loop regulates sugar homeostasis and grain yield in rice

Li, Zhiyong; Wei, Xiangjin; Tong, Xiaohong; Zhao, Juan; Liu, Xixi; Wang, Huimei; Tang, Liqun; Shu, Yazhou; Li, Guanghao; Wang, Yifeng; Ying, Jie‐Zheng; Jiao, Guiai; Hu, Honghong; Hu, Peisong; Jian, Zhang

doi:10.1016/j.molp.2022.01.016

Cited by 58 publications

(50 citation statements)

References 76 publications

(101 reference statements)

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“…The latex outflow increases significantly with each successive tapping until the yield stabilizes at ~10-fold that of the initial tapping after five to seven tappings [ 29 , 40 ]. The crucial roles of sucrose metabolism control in rubber biosynthesis and latex regeneration [ 41 , 42 ] and the presumed signaling roles of T6P in the regulation of sucrose allocation and utilization [ 8 , 18 ] prompted us to investigate whether T6P signaling participates in tapping-stimulated rubber production. As shown in Figure 5 A, T6P increased progressively with tapping, reaching 15.3 µM in the latex at the seventh tapping with more than a two-fold rise compared to the first tapping.…”

Section: Resultsmentioning

confidence: 99%

“…The mechanistic basis of T6P signaling has been investigated in plants, mainly in Arabidopsis. A close correlation between T6P and sucrose is observed both in wild-type and in transgenic Arabidopsis with changed expressions for TPS or TPP genes [ 18 , 19 ]. T6P seems to act as an indicator of sucrose status, and the T6P:sucrose ratio is critical for regulating sucrose levels within a range conducive to plant growth and development [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…A close correlation between T6P and sucrose is observed both in wild-type and in transgenic Arabidopsis with changed expressions for TPS or TPP genes [ 18 , 19 ]. T6P seems to act as an indicator of sucrose status, and the T6P:sucrose ratio is critical for regulating sucrose levels within a range conducive to plant growth and development [ 18 ]. However, more and more findings reveal that the role of T6P in signaling sucrose availability has a strong tissue and developmental context [ 8 , 15 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

“…This stimulus is especially conspicuous in previously untapped (‘virgin’) rubber trees. Taking cognizance of the close correlation between sucrose and T6P levels [ 18 ], changes in TPS -related expression, enzyme activity, and metabolite levels would be useful indicators as to how their functions are inter-related, and how they affect rubber production. Besides losing rubber, the hydrocarbon in the latex when the rubber tree is tapped, a large amount of water is lost from the laticifer system at the same time.…”

Section: Introductionmentioning

confidence: 99%

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Trehalose 6-Phosphate/SnRK1 Signaling Participates in Harvesting-Stimulated Rubber Production in the Hevea Tree

Zhou

Fang

Xiao

et al. 2022

Plants

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Trehalose 6-phosphate (T6P), the intermediate of trehalose biosynthesis and a signaling molecule, affects crop yield via targeting sucrose allocation and utilization. As there have been no reports of T6P signaling affecting secondary metabolism in a crop plant, the rubber tree Hevea brasiliensis serves as an ideal model in this regard. Sucrose metabolism critically influences the productivity of natural rubber, a secondary metabolite of industrial importance. Here, we report on the characterization of the T6P synthase (TPS) gene family and the T6P/SNF1-related protein kinase1 (T6P/SnRK1) signaling components in Hevea laticifers under tapping (rubber harvesting), an agronomic manipulation that itself stimulates rubber production. A total of fourteen TPS genes were identified, among which a class II TPS gene, HbTPS5, seemed to have evolved with a function specialized in laticifers. T6P and trehalose increased when the trees were tapped, this being consistent with the observed enhanced activities of TPS and T6P phosphatase (TPP) and expression of an active TPS-encoding gene, HbTPS1. On the other hand, SnRK1 activities decreased, suggesting the inhibition of elevated T6P on SnRK1. Expression profiles of the SnRK1 marker genes coincided with elevated T6P and depressed SnRK1. Interestingly, HbTPS5 expression decreased significantly with the onset of tapping, suggesting a regulatory function in the T6P pathway associated with latex production in laticifers. In brief, transcriptional, enzymatic, and metabolic evidence supports the participation of T6P/SnRK1 signaling in rubber formation, thus providing a possible avenue to increasing the yield of a valuable secondary metabolite by targeting T6P in specific cells.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Trehalose 6-Phosphate/SnRK1 Signaling Participates in Harvesting-Stimulated Rubber Production in the Hevea Tree

Zhou

Fang

Xiao

et al. 2022

Plants

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“…The grains of 93-11-OE were on average 24.8% longer, 8.6% narrower and 16.3% heavier, which theoretically lead to an average 46.2% increase in the grain yield per panicle ( Wang et al, 2016 ). OsNAC23 was overexpressed in Nanjing 46, and the transgenic lines exhibited increased panicle numbers, panicle size, and grain size and showed 11.46–12.56% yield increases compared with Nanjing 46 ( Li et al, 2022 ). In our study, qGL3.5 was knocked out in Huaye 3, and the grain size and panicle shape of the edited transgenic plants showed obvious changes.…”

Section: Discussionmentioning

confidence: 99%

Identification of qGL3.5, a Novel Locus Controlling Grain Length in Rice Through Bulked Segregant Analysis and Fine Mapping

et al. 2022

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Grain length (GL) directly affects the yield and quality of rice. Very few cloned GL-related genes are applied in production because their yield-increasing effects are not obvious, and the overall regulatory networks underlying the associated processes remain poorly understood. DNA samples from two bulk DNA pools (L-pool and S-pool) and their parents (KJ01 and Huaye 3) were subjected to high-throughput sequencing. Using bulked segregant analysis (BSA), qGL3.5 was mapped to a 0.34-Mb “hotspot” region on chromosome 3 that contains 37 genes related to various traits. Then, qGL3.5 was mapped to the genomic interval between the flanking markers M2 and M3 using 2786 BC4F2 individuals. Because the region from B5 to B6 was not the associated region under BSA-seq analysis, qGL3.5 was narrowed down to the interval between B6 and M3, which spanned 24.0-kb. Of all 37 genes with non-synonymous single-nucleotide polymorphisms (SNPs) between KJ01 and Huaye 3 based on BSA-seq analysis, only one complete annotated gene, ORF18 (Gene ID: LOC_Os03g42790.1) was found. ORF18 encodes an IBR-RING zinc-finger-related protein, with one really interesting new gene (RING) and two in between ring finger (IBR) domains. The knockout of ORF18 derived from Huaye 3 using clustered, regularly interspaced, short palindromic repeat (CRISPR)/CRISPR-associated 9 (Cas9) editing technology increased the GL of the mutant by approximately 2.2 mm. The novel locus qGL3.5 negatively regulated GL by promoting elongation of the longitudinal cell of the grain outer glume. These results provide a new genetic resource for rice grain shape breeding and a starting point for the functional characterization of the wild rice GL gene.

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A TaSnRK1α Modulates TaPAP6L‐Mediated Wheat Cold Tolerance through Regulating Endogenous Jasmonic Acid

Zhang,

Wang

et al. 2023

Advanced Science

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Here, a sucrose non‐fermenting‐1‐related protein kinase alpha subunit (TaSnRK1α‐1A) is identified as associated with cold stress through integration of genome‐wide association study, bulked segregant RNA sequencing, and virus‐induced gene silencing. It is confirmed that TaSnRK1α positively regulates cold tolerance by transgenes and ethyl methanesulfonate (EMS) mutants. A plastid‐lipid‐associated protein 6, chloroplastic‐like (TaPAP6L‐2B) strongly interacting with TaSnRK1α‐1A is screened. Molecular chaperone DJ‐1 family protein (TaDJ‐1‐7B) possibly bridged the interaction of TaSnRK1α‐1A and TaPAP6L‐2B. It is further revealed that TaSnRK1α‐1A phosphorylated TaPAP6L‐2B. Subsequently, a superior haplotype TaPAP6L‐2B30S/38S is identified and confirmed that both R30S and G38S are important phosphorylation sites that influence TaPAP6L‐2B in cold tolerance. Overexpression (OE) and EMS‐mutant lines verified TaPAP6L positively modulating cold tolerance. Furthermore, transcriptome sequencing revealed that TaPAP6L‐2B‐OE lines significantly increased jasmonic acid (JA) content, possibly by improving precursor α‐linolenic acid contributing to JA synthesis and by repressing JAR1 degrading JA. Exogenous JA significantly improved the cold tolerance of wheat plants. In summary, TaSnRK1α profoundly regulated cold stress, possibly through phosphorylating TaPAP6L to increase endogenous JA content of wheat plants.

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The OsNAC23-Tre6P-SnRK1a feed-forward loop regulates sugar homeostasis and grain yield in rice

Cited by 58 publications

References 76 publications

Trehalose 6-Phosphate/SnRK1 Signaling Participates in Harvesting-Stimulated Rubber Production in the Hevea Tree

Trehalose 6-Phosphate/SnRK1 Signaling Participates in Harvesting-Stimulated Rubber Production in the Hevea Tree

Identification of qGL3.5, a Novel Locus Controlling Grain Length in Rice Through Bulked Segregant Analysis and Fine Mapping

A TaSnRK1α Modulates TaPAP6L‐Mediated Wheat Cold Tolerance through Regulating Endogenous Jasmonic Acid

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