The Os-AKT1 Channel Is Critical for K<sup>+</sup> Uptake in Rice Roots and Is Modulated by the Rice CBL1-CIPK23 Complex

Li, Juan; Long, Yu; Gao, Qi; Xu, Zijian; Wu, Weihua; Wang, Yi

doi:10.1105/tpc.114.123455

Cited by 245 publications

(262 citation statements)

References 56 publications

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“…5). Importantly, activation of the rice (Oryza sativa) OsAKT1 (Li et al, 2014) or the grapevine (Vitis vinifera) VvK1.1 channels (Cuéllar et al, 2010) by CIPK23/CBL1 has also been described. Thus, the important regulatory role on HAK5-and AKT1-mediated K + uptake shown here for the Arabidopsis CIPK23/CBL complex can be extended to other plant species and, most importantly, to crops.…”

Section: Discussionmentioning

confidence: 99%

CIPK23 regulates HAK5-mediated high-affinity K+ uptake in Arabidopsis roots

et al. 2015

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Section: Discussionmentioning

confidence: 99%

CIPK23 regulates HAK5-mediated high-affinity K+ uptake in Arabidopsis roots

et al. 2015

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“…S11). Previous electrophysiological analyses suggested that AtKC1 function in rice is weak (Fuchs et al, 2005;Li et al, 2014), although two putative AtKC1 homologs have been found in the rice genome (Supplemental Fig. S11).…”

Section: Discussion Physiological Function Of Atkc1 In Plant Cellsmentioning

confidence: 99%

“…This CBL1/ 9-CIPK23-AKT1 pathway is an important mechanism in the Arabidopsis response to LK stress. A similar mechanism was also identified in other plant species (for review, see Véry et al, 2014), such as rice (Oryza sativa; Li et al, 2014). Subsequently, a 2C-type protein phosphatase, AIP1, was identified that interacts with and inactivates the AKT1 channel (Lee et al, 2007).…”

mentioning

confidence: 90%

AtKC1 and CIPK23 Synergistically Modulate AKT1-Mediated Low-Potassium Stress Responses in Arabidopsis

et al. 2016

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In Arabidopsis (Arabidopsis thaliana), the Shaker K + channel AKT1 conducts K + uptake in root cells, and its activity is regulated by CBL1/9-CIPK23 complexes as well as by the AtKC1 channel subunit. CIPK23 and AtKC1 are both involved in the AKT1-mediated low-K + (LK) response; however, the relationship between them remains unclear. In this study, we screened suppressors of low-K + sensitive [lks1 (cipk23)] and isolated the suppressor of lks1 (sls1) mutant, which suppressed the leaf chlorosis phenotype of lks1 under LK conditions. Map-based cloning revealed a point mutation in AtKC1 of sls1 that led to an amino acid substitution (G322D) in the S6 region of AtKC1. The G322D substitution generated a gain-of-function mutation, AtKC1 D , that enhanced K + uptake capacity and LK tolerance in Arabidopsis. Structural prediction suggested that glycine-322 is highly conserved in K + channels and may function as the gating hinge of plant Shaker K + channels. Electrophysiological analyses revealed that, compared with wild-type AtKC1, AtKC1 D showed enhanced inhibition of AKT1 activity and strongly reduced K + leakage through AKT1 under LK conditions. In addition, phenotype analysis revealed distinct phenotypes of lks1 and atkc1 mutants in different LK assays, but the lks1 atkc1 double mutant always showed a LK-sensitive phenotype similar to that of akt1. This study revealed a link between CIPK-mediated activation and AtKC1-mediated modification in AKT1 regulation. CIPK23 and AtKC1 exhibit distinct effects; however, they act synergistically and balance K + uptake/leakage to modulate AKT1-mediated LK responses in Arabidopsis.

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“…The slope for each K + concentration was calculated according to the linear regression of the growth curves during the logarithmic growth phase. The curve was obtained by applying nonlinear regression analysis using the MichaelisMenten equation (Horie et al 2011;Li et al 2014). …”

Section: Yeast Complementation Assaysmentioning

confidence: 99%

The inward-rectifying K+ channel SsAKT1 is a candidate involved in K+ uptake in the halophyte Suaeda salsa under saline condition

Duan

Zhang

et al. 2015

Plant Soil

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Background and aims The Shaker AKT1-like channels are considered to be involved in both high-and lowaffinity K + uptake and correlated with salt tolerance in glycophytes. Suaeda salsa (Suaeda maritima subsp. salsa), as a typical salt-accumulating halophyte, is able to absorb K + efficiently while growing under saline conditions and taking in a large amount of Na + , thus maintaining the K + concentration in its cells. In this study, the possible functions of the inward-rectifying K + channel SsAKT1 in K + uptake and salt tolerance in the halophyte S. salsa were investigated.Methods SsAKT1 from S. salsa was isolated by RT-PCR and characterized using yeast complementation; the responses of SsAKT1 to various KCl and NaCl treatments were investigated by real-time quantitative PCR. Results SsAKT1 consisted of 879 amino acid residues and shared high homology (60-67 %) with the identified inward-rectifying K + channels AKT1 from other plants. The expression of SsAKT1 rescued the K + -uptake-defective phenotype of yeast strain CY162, and also suppressed the salt-sensitive phenotype of yeast strain G19, suggesting SsAKT1 functioned as an inward-rectifying K + channel. SsAKT1 was predominantly expressed in roots, and was induced significantly by K + starvation; transcript levels increased further on resupply of K + (0.1-10 mM for 6 h) by 62 % in 0.1 mM K + and 144-174 % in higher K + concentrations (1-10 mM). Interestingly, the expression level of SsAKT1 in roots was also induced significantly by short-term treatment (6 h) with NaCl concentrations (25-250 mM). Conclusions These results demonstrate that the inwardrectifying K + channel SsAKT1 might mediate both high-and low-affinity K + uptake in S. salsa, but play a greater role in the low-affinity system. Furthermore, SsAKT1 might also be involved in salt tolerance by participating in the maintenance of K + nutrition in S. salsa under salinity.

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The Os-AKT1 Channel Is Critical for K⁺ Uptake in Rice Roots and Is Modulated by the Rice CBL1-CIPK23 Complex

Cited by 245 publications

References 56 publications

CIPK23 regulates HAK5-mediated high-affinity K+ uptake in Arabidopsis roots

CIPK23 regulates HAK5-mediated high-affinity K+ uptake in Arabidopsis roots

AtKC1 and CIPK23 Synergistically Modulate AKT1-Mediated Low-Potassium Stress Responses in Arabidopsis

The inward-rectifying K+ channel SsAKT1 is a candidate involved in K+ uptake in the halophyte Suaeda salsa under saline condition

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The Os-AKT1 Channel Is Critical for K+ Uptake in Rice Roots and Is Modulated by the Rice CBL1-CIPK23 Complex

Cited by 245 publications

References 56 publications

CIPK23 regulates HAK5-mediated high-affinity K+ uptake in Arabidopsis roots

CIPK23 regulates HAK5-mediated high-affinity K+ uptake in Arabidopsis roots

AtKC1 and CIPK23 Synergistically Modulate AKT1-Mediated Low-Potassium Stress Responses in Arabidopsis

The inward-rectifying K+ channel SsAKT1 is a candidate involved in K+ uptake in the halophyte Suaeda salsa under saline condition

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The Os-AKT1 Channel Is Critical for K⁺ Uptake in Rice Roots and Is Modulated by the Rice CBL1-CIPK23 Complex