The origin of the voltage dependence of conductance blockades from DNA translocation through solid-state nanopores

Zhang, Yin; Lian, Xiang; Si, Wei; Sha, Jingjie; Chen, Yunfei

doi:10.1039/d3qm00699a

Cited by 2 publications

(2 citation statements)

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“…The linear configuration type, which had a small blocked current amplitude, occupies the majority of events, and more linear translocation events occurred at a higher applied voltage. 40 To eliminate the impact of the DNA translocation configuration, we considered only the transport behavior of linear translocation events in this work. Theoretically, an increase in the angle of the nanopore raises the probability that the DNA collides with the nanopore and friction occurs.…”

Section: Resultsmentioning

confidence: 99%

Confined Transport Behavior of Biomolecules within Tilted Nanopores

Ma,

Xu,

Liu

et al. 2024

J. Phys. Chem. B

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The transport behavior of biomolecules at the confined nanoscale is very different from that of the bulk state. Numerous disease diagnostics and targeted drug treatments are performed based on nanochannels in cells. The specific structure and shape of nanochannels play an important role in the behavior and efficiency of substance transport. In this paper, we fabricated nanopores with different tilt angles and the same diameters using focused ion beam. The capture frequency and the blocking current amplitude of λ-DNA within large-angle nanopores decrease obviously, suggesting an increase in the energy barrier of large-angle nanopores and the fact that they stretch biomolecules to thinness. Most importantly, large-angle nanopores slow down λ-DNA transport by 2−4 times. MD simulations find that the sloped electroosmotic flow inside the tilted nanopores is the main factor contributing to the transport phenomena. The increase in the capture time of biomolecules by nanopores assists in obtaining more biological information from the current trajectories. Our study provides a new understanding of substance transport in specially shaped nanopores, which can be instrumental in providing fresh inspiration and approaches to the biomedical field.

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Section: Resultsmentioning

confidence: 99%

Confined Transport Behavior of Biomolecules within Tilted Nanopores

Ma,

Xu,

Liu

et al. 2024

J. Phys. Chem. B

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“…The surface charge uniformly distributed led to the sharp changes of the K + ion concentration next to the left and right sides of the nanobubble, and this high concentration was attributed to the diminished cross-section area of the nanopore . The enhancement of K + ion concentration became more pronounced under higher voltages . Consequently, the higher K + concentration in the pore resulted in increased electrostatic adsorption on the nanobubble surface, leading to a lower surface charge density of the nanobubble (neutralization of negative charge OH – ).…”

Section: Resultsmentioning

confidence: 99%

Single-Digit Nanobubble Sensing via Nanopore Technology

Liu,

Zheng,

et al. 2024

Anal. Chem.

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Nanobubbles play an important role in diverse fields, including engineering, medicine, and agriculture. Understanding the characteristics of individual nanobubbles is essential for comprehending fluid dynamics behaviors and advancing nanoscale science across various fields. Here, we report a strategy based on nanopore sensors for characterizing single-digit nanobubbles. We investigated the sizes and diffusion coefficients of nanobubbles at different voltages. Additionally, the finite element simulation and molecular dynamics simulation were introduced to account for counterion concentration variation around nanobubbles in the nanopore. In particular, the differences in stability and surface charge density of nanobubbles under various solution environments have been studied by the ion-stabilized model and the DLVO theory. Additionally, a straightforward method to mitigate nanobubble generation in the bulk for reducing current noise in nanopore sensing was suggested. The results hold significant implications for enhancing the understanding of individual nanobubble characterizations, especially in the nanofluid field.

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The origin of the voltage dependence of conductance blockades from DNA translocation through solid-state nanopores

Abstract: Understanding the origin of ionic conductance modulation of nanopore sensors is important for achieving the precise measurements of analytes at single molecule level. Most reported model of the amplitude of...

Cited by 2 publications

References 41 publications

Confined Transport Behavior of Biomolecules within Tilted Nanopores

Confined Transport Behavior of Biomolecules within Tilted Nanopores

Single-Digit Nanobubble Sensing via Nanopore Technology

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