The origin of the long-wavelength fluorescence emission band (77°K) from photosystem I

Kuang, Tingyun; Argyroudi‐Akoyunoglou, J. H.; Nakatani, H.Y.; Watson, Janet L.; Arntzen, Charles J.

doi:10.1016/0003-9861(84)90236-4

Cited by 78 publications

(36 citation statements)

References 18 publications

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“…Moreover, a major difference is evident in the dissociation pattern of LHCI from plants and algae. In C. reinhardtii a single complex of about 200 kDa, called CPO, is found containing all the LHCI polypeptides (Wollmann and Bennoun, 1982;Herrin et al, 1987); in contrast, the two complexes obtained from higher plants migrate, at around 43 kDa (LHCI 730) (Kuang et al, 1984;Lam et a/., 1984a;Remy and Ambart-Bretteville, 1984;Sarvari et al , 1985, Bassi et al, 1985 superimposing on the Chl a binding protein of PSI1 (CP43) (Metz et al, 1984;Bassi, 1985;Dunahay and Staehelin, 1985), and the other (LHCI-680) to the same MW region as the monomeric form of LHCII (Bassi et al, 1985;Bassi and Simpson, 1987).…”

Section: Chlorophyll Alb Proteins Of Photosystem Imentioning

confidence: 99%

CHLOROPHYLL BINDING PROTEINS WITH ANTENNA FUNCTION IN HIGHER PLANTS and GREEN ALGAE

Bassi

Rigoni

Giacometti

1990

Photochem & Photobiology

158

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Section: Chlorophyll Alb Proteins Of Photosystem Imentioning

confidence: 99%

CHLOROPHYLL BINDING PROTEINS WITH ANTENNA FUNCTION IN HIGHER PLANTS and GREEN ALGAE

Bassi

Rigoni

Giacometti

1990

Photochem & Photobiology

158

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“…Thus, the CPIa/CPI ratio drops from a value of 0.6 (24 h CL) to 0.25 (24 h CL + 48 h D). The CPIa is a highly organized complex derived from the PSI unit, containing the core complex CPI, and the light-harvesting antenna complex ofPSI, LHC-I (7,23). The CPla can be easily dissociated into its components, CPI and LHC-I (7,23).…”

mentioning

confidence: 99%

“…The CPIa is a highly organized complex derived from the PSI unit, containing the core complex CPI, and the light-harvesting antenna complex ofPSI, LHC-I (7,23). The CPla can be easily dissociated into its components, CPI and LHC-I (7,23). The decrease, therefore, in the concentration ofthe CPIa during the dark period may be due to the decrease of the LHC-I.…”

mentioning

confidence: 99%

“…It has been shown (12) that there is a good correlation between the changes in the 77°K fluorescence emission spectra of developing plastids and the step-wise synthesis, assembly and organization of the pigment-protein complexes; moreover, that the detection of the 730 to 735 nm band depends on the synthesis of LHC-I and its incorporation along with CPI into CPIa (7,12,23). For example, the intermittent light leaves contain chloroplasts which have only the core complexes of the PSII and PSI units (CPa and CPI, respectively).…”

mentioning

confidence: 99%

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Reorganization of Thylakoid Components during Chloroplast Development in Higher Plants after Transfer to Darkness

Akoyunoglou

Akoyunoglou²

1985

Plant Physiol.

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It was shown earlier that in etiolated bean (Phaseolus rulgaris, var. red kidney) leaves exposed to continuous light for a short time and then transferred to darkness a reorpnization of their photosystem II (PSII) unit components occurs. This reorpnization involves disornization of the light-harvesting complex of PSII (LHC-II), destruction of its chlorophyll b and the 25 kilodalton polypeptide, and reuse of its chlorophyll a for the formation of additional, small in size, PSII units (ArgyroudiAkoyunoglou, Akoyunoglou, Kaloskas, Akoyunoglou 1982 Plant Physiol 70: 1242-1248. The present study further shows that parallel to the PSII unit reorganization a reorganization of the PSI unit components also occurs: upon transfer to darkness the 24, 23, and 21 kilodalton polypeptides, components of the light-harvesting complex of PSI (LHC-I), are decreased, the 69 kilodalton polypeptide, component of the chlorophyll a-rich P700-protein complex (CPI), is increased and new smallsized PSI units are formed. Concomitantly, the cytochromeJlchlorophyll and the cytochrome b/chlorophyll ratios are gradually increased. This suggests that the concentration of the electron transport components is also modulated in darkness to allow for adequate electron flow to occur between the newly synthesized PSII and PSI units.Young etiolated bean leaves exposed to CL2 accumulate Chl a and Chl b without lag-phase (3), and they form complete (large in size) photosynthetic units. The concentration of the PSII and PSI units per chloroplast increases with the time of exposure to CL, until it reaches the concentration of the mature chloroplast.Under certain conditions, but always in cases where the thylakoid is still in the process of development, the thylakoid components seem to undergo a process of reorganization (2, 4). Such a case has been noticed recently in the PSII unit components of etiolated bean leaves exposed to CL for a short time and then transferred to darkness (1 1): after transfer to darkness Chl accumulation stops completely; however, the growth of the leaf continues, and thus the Chl per g fresh weight decreases.

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“…Whether CPy bears any relation to the PSI antenna complex of higher plants (referred to as LHC I or LHCP I) remains to be determined. CPy of Euglena, like LHC I of higher plants (2,17,21,23), migrates close to the LHCP2 complex (Fig. 3), and contains pigments (Table II) and, perhaps, polypeptides (10) also found in the CP Ia complex.…”

Section: Resultsmentioning

confidence: 90%

Chlorophyll-Protein Complexes from Euglena gracilis and Mutants Deficient in Chlorophyll b

Cunningham¹,

Schiff²

1986

Plant Physiol.

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The use of n-octyl-ft-n-glucopyranoside along with sodium dodecyl sulfate improves the retention of chlorophyll (Chl) by chlorophyll-protein complexes (CPs) prepared from thylakoids of Euglena grailis Klebs var bacillaris Cori and yields several additional complexes. Thylakoids from wild-type (WT) cells, solubilized in these detergents and subjected to polyacrylamide gel electrophoresis at 0°C, yield the following CPs, in order of relative molecular weight, containing the pigments shown in parentheses with their respective molar ratios where determined: CP Ia (Chi a, diadinoxanthin and 0-carotene; 100:12:5); CP I (Chi a and 0-carotene; 100:6-12); CPx (Chi and carotenoids); LHCP2 (light-harvesting CP oligomer) (Chl a, Chl b, diadinoxanthin and neoxanthin; 12:4:3:1); CPy (Chl a, diadinoxanthin and 0-carotene; 100:14:8); CPa (Chi a and 0-carotene; 100:18-25) and LHCP (monomer) (Chl a, Chl b, diadinoxanthin and neoxanthin; 12:6:4:1). The [30] for nomenclature) were cultured in I L batches of Hutner's pH 3.5 medium (15) at 26°C. In most cases, the 2 L Erlenmeyer flasks containing the cultures were placed on a rotary platform shaker adjusted to 110 cycles/ min and were illuminated from above at a fluence rate of 4.0 W/m2 provided by alternating red and cool-white fluorescent lamps (Sylvania) screened with a plastic mesh to reduce the light intensity. For some experiments, cultures were grown without shaking (standing) on a glass shelf and were illuminated from above and below at a fluence rate of 14.0 W/m2 with the unscreened fluorescent lamps described above. All cultures were harvested 2 d after cell division had ceased; at this point the cellular Chl contents had reached stable values. Cell density was determined with a Coulter model Zb cell counter (100 AM aperture).Preparation of Thylakoids. Cultures (usually 10-20 L) of Euglena were filtered through four layers of cheesecloth and the cells were pelleted by centrifugation at 100g for 2 min at 4°C.All subsequent procedures were carried out at 4°C or on ice. The cell pellets were combined, washed with ice-cold distilled H20, and resuspended in a BS containing 0.3 M sucrose and 50 mm Tris-HCl at pH 7.6. Cells were pelleted by centrifugation at 1 640g for 5 min in preweighed centrifuge bottles, the supernatant fluid was removed by aspiration, and the weights of the pellets were determined. BS containing 1.0 mM PMSF (Sigma) was 223 www.plantphysiol.org on May 10, 2018 -Published by Downloaded from

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The origin of the long-wavelength fluorescence emission band (77°K) from photosystem I

Cited by 78 publications

References 18 publications

CHLOROPHYLL BINDING PROTEINS WITH ANTENNA FUNCTION IN HIGHER PLANTS and GREEN ALGAE

CHLOROPHYLL BINDING PROTEINS WITH ANTENNA FUNCTION IN HIGHER PLANTS and GREEN ALGAE

Reorganization of Thylakoid Components during Chloroplast Development in Higher Plants after Transfer to Darkness

Chlorophyll-Protein Complexes from Euglena gracilis and Mutants Deficient in Chlorophyll b

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