The Origin of Red Algae and Cryptomonad Nucleomorphs: A Comparative Phylogeny Based on Small and Large Subunit rRNA Sequences ofPalmaria palmata, Gracilaria verrucosa,and theGuillardia thetaNucleomorph

Auwera, Gert Van der; Hofmann, Chr; Rijk, Peter De; Wächter, Rupert De

doi:10.1006/mpev.1998.0544

Cited by 25 publications

(10 citation statements)

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“…Cryptomonad nucleomorph 18S rDNA, which has been diverging faster than nuclear 18S rDNA, may be appropriate and has already been used to some extent for cryptomonad phylogeny (Cavalier‐Smith et al 1996). However, a shortcoming of using nucleomorph sequence is that the evolutionary distance between the cryptomonad endosymbiont and the red algae, from which it apparently arose, is large and will make outgroup rooting of the cryptomonad clade difficult (Cavalier‐Smith et al 1996, Van der Auwera et al 1998). Another possibility would be to use a combined 18S/28S nuclear rDNA data set to give greater phylogenetic resolution.…”

Section: Discussionmentioning

confidence: 99%

CRYPTOMONAD EVOLUTION: NUCLEAR 18S rDNA PHYLOGENY VERSUS CELL MORPHOLOGY AND PIGMENTATION¹

Deane

Strachan

Saunders

et al. 2002

Journal of Phycology

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A nuclear18S rDNA phylogeny for cryptomonad algae is presented, including 11 species yet to be investigated by molecular means. The phylogenetic positions of the cryptomonad genera Campylomonas and Plagioselmis are assessed for the first time. Campylomonas groups most closely with morphologically similar species with the same accessory pigment from the genus Cryptomonas. Plagioselmis groups with the genera Teleaulax and Geminigera forming a clade whose members are united by unusual thylakoid arrangement. Nuclear 18S rDNA phylogeny divides cryptomonads into seven major lineages, two of which consist of the monospecific genera Proteomonas and Falcomonas. Analysis of nuclear18S rDNA sequence supports suggestions that a Falcomonas‐like cryptomonad gave rise to all other blue‐green cryptomonads. New sequence from the plastid‐lacking cryptomonad genus Goniomonas is also included, and the order of divergence of the major cryptomonad lineages is discussed. The morphology, number, and pigmentation of the cryptomonad plastidial complex are congruent with nuclear 18S rDNA phylogenies. Host cell features, such as periplast type, furrow/gullet system, and cell shape, can be more variable and may be markedly different in species that are closely related by nuclear 18S rDNA phylogeny. Conversely, some species that are not closely related by molecular phylogeny may display a very similar, possibly primitive, periplast and furrow morphology.

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Section: Discussionmentioning

confidence: 99%

CRYPTOMONAD EVOLUTION: NUCLEAR 18S rDNA PHYLOGENY VERSUS CELL MORPHOLOGY AND PIGMENTATION¹

Deane

Strachan

Saunders

et al. 2002

Journal of Phycology

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“…PCR and sequencing primers are listed in Table 2. Secondary structure information was used to align sequence data from this study with two previously published LSU sequences (Van der Auwera et al 1998). The resulting multiple alignment was modified by eye, and ambiguous regions were removed, including an intron in Hildenbrandia rubra.…”

Section: Methodsmentioning

confidence: 99%

MOLECULAR SYSTEMATICS OF THE FLORIDEOPHYCEAE (RHODOPHYTA) USING NUCLEAR LARGE AND SMALL SUBUNIT rDNA SEQUENCE DATA

Harper

Saunders

2001

Journal of Phycology

104

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Sequence data are presented for approximately 85% of the nuclear large subunit (LSU) rDNA gene for one member of the Bangiophyceae and 47 members of the Florideophyceae, the latter representing all but one of the currently recognized florideophyte orders. Distance, parsimony, and maximum likelihood analyses of these data were used to generate phylogenetic trees, and bootstrap resampling was implemented to infer robustness for distance and parsimony results. LSU phylogenies were congruent with published nuclear small subunit (SSU) rDNA results in that four higher level florideophyte lineages were resolved: lineage 1, containing the order Hildenbrandiales; lineage 2, recovered only under distance analysis, composed of the orders Acrochaetiales, Balliales, Batrachospermales, Corallinales, Nemaliales, Palmariales, and Rhodogorgonales; lineage 3, containing the Ahnfeltiales; and lineage 4, composed of the orders Bonnemaisoniales, Ceramiales, Gelidiales, Gigartinales, Gracilariales, Halymeniales, Plocamiales, and Rhodymeniales. Analyses were also performed on a combined LSU–SSU data set and an SSU‐only data set to account for differences in taxon sampling relative to published studies using this latter gene. Combined LSU–SSU analyses resulted in phylogenetic trees of similar topology and support to those obtained from LSU‐only analyses. Phylogenetic trees produced from SSU‐only analyses differed somewhat in particulars of branching within lineages 2 and 4 but overall were congruent with the LSU‐only and combined LSU–SSU results. We close with a discussion of the phylogenetic potential that the LSU has displayed thus far for resolving relationships within the Florideophyceae.

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“…11 This alignment was useful to define the different portions of the ITS region, and the sequences have been submitted to the EMBL database. The length of the 5.8S rRNA gene is almost constant for these three red algae, about 160 bp, slightly longer than for other Rhodophyta where a majority of 153-154 bp 5.8S rRNA genes have been noted by database sequence analysis (Table 2).…”

Section: Pcr Amplification and Sequencingmentioning

confidence: 99%

Species identification of red and brown seaweeds using ITS ribosomal DNA amplification and RFLP patterns

Antoine

Fleurence

2003

J Sci Food Agric

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The identity of five macroalgae used as sea vegetables or food ingredients has been determined by amplification of the ribosomal DNA ITS region and RFLP analysis. This allows the detection of specificity patterns for each species and provides an alternative method, when morphological or biochemical methods fail, for control of their use as food ingredients. Alga-specific PCR primers have been used to determine the ITS rDNA sequences of DNAs extracted from dried and ground algae up to 5 years old. The seaweeds studied were the red algae Palmaria palmata (dulse), Porphyra umbilicalis (nori), and Chondrus crispus (pioca) and the brown algae Himanthalia elongata (sea spaghetti) and Laminaria sp (konbu). Total genomic DNA suitable for amplification was extracted from the alga powder following the CTAB method. This methodology allowed the sequencing of the amplified product and the drawing of theoretical restriction maps useful in the choice of restriction enzymes for RFLP analysis. Enzymes that appeared useful included Mbo I and Alu I. Cutting with a single enzyme was sufficient to obtain characteristic patterns for the red algae P palmata, P umbilicalis and C crispus. For the two brown algae H elongata and Laminaria sp the ITS rDNA sequence showed a lack of suitable restriction site, contrary to other species for which characteristic patterns were obtained.

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The Origin of Red Algae and Cryptomonad Nucleomorphs: A Comparative Phylogeny Based on Small and Large Subunit rRNA Sequences ofPalmaria palmata, Gracilaria verrucosa,and theGuillardia thetaNucleomorph

Cited by 25 publications

References 47 publications

CRYPTOMONAD EVOLUTION: NUCLEAR 18S rDNA PHYLOGENY VERSUS CELL MORPHOLOGY AND PIGMENTATION¹

CRYPTOMONAD EVOLUTION: NUCLEAR 18S rDNA PHYLOGENY VERSUS CELL MORPHOLOGY AND PIGMENTATION¹

MOLECULAR SYSTEMATICS OF THE FLORIDEOPHYCEAE (RHODOPHYTA) USING NUCLEAR LARGE AND SMALL SUBUNIT rDNA SEQUENCE DATA

Species identification of red and brown seaweeds using ITS ribosomal DNA amplification and RFLP patterns

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The Origin of Red Algae and Cryptomonad Nucleomorphs: A Comparative Phylogeny Based on Small and Large Subunit rRNA Sequences ofPalmaria palmata, Gracilaria verrucosa,and theGuillardia thetaNucleomorph

Cited by 25 publications

References 47 publications

CRYPTOMONAD EVOLUTION: NUCLEAR 18S rDNA PHYLOGENY VERSUS CELL MORPHOLOGY AND PIGMENTATION1

CRYPTOMONAD EVOLUTION: NUCLEAR 18S rDNA PHYLOGENY VERSUS CELL MORPHOLOGY AND PIGMENTATION1

MOLECULAR SYSTEMATICS OF THE FLORIDEOPHYCEAE (RHODOPHYTA) USING NUCLEAR LARGE AND SMALL SUBUNIT rDNA SEQUENCE DATA

Species identification of red and brown seaweeds using ITS ribosomal DNA amplification and RFLP patterns

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Product

Resources

About

CRYPTOMONAD EVOLUTION: NUCLEAR 18S rDNA PHYLOGENY VERSUS CELL MORPHOLOGY AND PIGMENTATION¹

CRYPTOMONAD EVOLUTION: NUCLEAR 18S rDNA PHYLOGENY VERSUS CELL MORPHOLOGY AND PIGMENTATION¹