The origin of cytoplasmic inclusions from the nuclear envelope of mammalian oocytes

Baker, T. G.; Franchi, L. L.

doi:10.1007/bf00325022

Cited by 32 publications

(15 citation statements)

References 22 publications

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“…The term “nuclear bleb” has been used to describe a number of different nuclear membrane extensions, perhaps first used to describe evaginations of the inner membrane of the nuclear envelope in the pronuclei of rat and rabbit zygotes (Szollosi, 1965; Gulyas, 1971). Nuclear blebbing has also been described in oocytes from cattle, monkeys and humans (Baker and Franchi, 1969; Szollosi and Szollosi, 1988). The nuclear membrane blebs characterized here appear to differ structurally from most, if not all, previously described nuclear membrane blebs in germ cells.…”

Section: Discussionmentioning

confidence: 96%

Assembly of RNP granules in stressed and aging oocytes requires nucleoporins and is coordinated with nuclear membrane blebbing

Patterson

Wood

Schisa

2011

Developmental Biology

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Protective cellular responses to stress and aging in the germ line are essential for perpetuation of a species; however, relatively few studies have focused on how germ cells respond to stress and aging. We have previously shown that large ribonucleoprotein (RNP) complexes assemble in oocytes of Caenorhabditis during extended meiotic arrest or after environmental stress. Here we explore the regulation of these dynamic RNPs and demonstrate their assembly is coordinated with dramatic, nuclear membrane blebbing in oocytes. Our ultrastructural analyses reveal distinct changes in the endoplasmic reticulum, and the first evidence for the assembly of stacked annulate lamellae in Caenorhabditis. We further show several nucleoporins are required for the complete assembly of RNP granules, and a disruption in RNP granule assembly coupled with a low frequency of nuclear blebbing in arrested oocytes negatively impacts embryonic viability. Our observations support a model where nuclear membrane blebbing is required to increase the trafficking of nucleoporins to the cell cortex in stressed or meiotically-arrested cells and to facilitate the recruitment of RNA and protein components of RNPs into large complexes. These new insights may have general implications for better understanding how germ cells preserve their integrity when fertilization is delayed and how cells respond to stress.

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Section: Discussionmentioning

confidence: 96%

Assembly of RNP granules in stressed and aging oocytes requires nucleoporins and is coordinated with nuclear membrane blebbing

Patterson

Wood

Schisa

2011

Developmental Biology

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“…The association of a large mitochondrion with the kinetoplast of trypansomes may be a corresponding process (Steinert, 1961). In animal cells in situ and in culture intimate relationships between mitochondria and the nuclei are common (for review see Bereiter-Hahn, 1990) which has been misinterpreted as indicating a de novo formation of mitochondria from the nuclear envelope (for review see, e.g., Baker and Franchi, 1969;Roodyn and Wilkie, 1968). Ample evidence was brought forward for the association of mitochondria with the rough endoplasmic reticulum (rER) and with the plasma membrane (for review see, e.g., Bereiter-Hahn, 1990).…”

Section: Mitochondrial Associations With Energy Consuming Structuresmentioning

confidence: 99%

Dynamics of mitochondria in living cells: Shape changes, dislocations, fusion, and fission of mitochondria

Bereiter‐Hahn

Vöth

1994

Microscopy Res & Technique

782

496

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Mitochondria are semi-autonomous organelles which are endowed with the ability to change their shape (e.g., by elongation, shortening, branching, buckling, swelling) and their location inside a living cell. In addition they may fuse or divide. These dynamics are discussed. Dislocation of mitochondria may result from their interaction with elements of the cytoskeleton, with microtubules in particular, and from processes intrinsic to the mitochondria themselves. Morphological criteria and differences in the fate of some mitochondria argue for the presence of more than one mitochondrial population in some animal cells. Whether these reflect genetic differences remains obscure. Emphasis is laid on the methods for visualizing mitochondria in cells and following their behaviour. Fluorescence methods provide unique possibilities because of their high resolving power and because some of the mitochondria-specific fluorochromes can be used to reveal the membrane potential. Fusion and fission often occur in short time intervals within the same group of mitochondria. At sites of fusion of two mitochondria material of the inner membrane, the matrix compartment seems to accumulate. The original arrangement of the fusion partners is maintained for some minutes. Fission is a dynamic event which, like fusion, in most cases observed in vertebrate cell cultures is not a straight forward process but rather requires several "trials" until the division finally occurs. Regarding fusion and fission hitherto unpublished phase contrast micrographs, and electron micrographs have been included.

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“…This describes the formation of nuclear outpocketings, followed by membrane breakage and fusion in the neck region of this nuclear bleb, and detachment of a nuclear envelope-surrounded bit of nuclear contents. This seems to be the chief mechanism by which nucleolar material and nucleolar remnants are extruded from the nuclei of various gland cells and oocytes, and from pronuclei (Baud, 1953;Gay, 1955Gay, , 1956Clark, 1960;Berendes and DeBruyn, 1963;Kessel and Beams, 1963;Bell and Miihlethaler, 1964;Szollosi, 1965;Baker and Franchi, 1969;Lima-de-Faria, 1971). After…”

Section: Large Molecules and Particlesmentioning

confidence: 99%

“…somehow attached to it (e.g. Ornstein, 1956;Drawert and Mix, 1961;Balinsky and Devis, 1963;Meyer, 1963;Baker and Franchi, 1969;Hsu, 1967;Kessel, 1968b;Aikawa et al, 1970;Rowley et al, 1971; for further references see , as demonstrable by the tenacity with which they stick to isolated nuclei and even to the envelopes. Such an interaction might be effected simply by hydrophobic surface adsorption, but structures possibly involved in a direct fixation of such nuclear surface-mitochondria associations are also noticeable.…”

Section: Relationship Of the Nuclear Envelope To Other Cell Organmentioning

confidence: 99%

Structures and Functions of the Nuclear Envelope

Franke¹,

Scheer²

1974

The Cell Nucleus

153

150

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The origin of cytoplasmic inclusions from the nuclear envelope of mammalian oocytes

Cited by 32 publications

References 22 publications

Assembly of RNP granules in stressed and aging oocytes requires nucleoporins and is coordinated with nuclear membrane blebbing

Assembly of RNP granules in stressed and aging oocytes requires nucleoporins and is coordinated with nuclear membrane blebbing

Dynamics of mitochondria in living cells: Shape changes, dislocations, fusion, and fission of mitochondria

Structures and Functions of the Nuclear Envelope

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