The ori sequences of the mitochondrial genome of a wild-type yeast strain: number, location, orientation and structure

Zamaroczy, Miklos de; Faugeron-Fonty, Godeleine; Baldacci, Giuseppe; Goursot, Regina; Bernardi, Giorgio

doi:10.1016/0378-1119(84)90019-2

Cited by 86 publications

(41 citation statements)

References 54 publications

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“…This sequence not only corresponds to that of the ARS consensus but, moreover, matches perfectly the highly conserved 11 bp sequence found in the la region of S. cerevisiae oril, ori5, and ori [30] and the ARS element of C. utilis mtDNA [lo]. Overlapping this ARS site, in the strand complementary to that shown in fig.3, there is a 9-nucleotide-long sequence ATATAAATA which shows striking homology with an mtDNA transcription initiation signal in yeast -ATATAAGTA [3 11.…”

Section: Resultssupporting

confidence: 74%

Structure of a Cephalosporium acremonium mtDNA replicator

Peñalva¹,

Patiño²,

Rubio³

1986

FEBS Letters

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We have investigated the ARS (~~to~~rno~~~ repliiating sequence) activity of a I.94 kb ~~to~~o~dr~~l DNA fragmeut of ~~~~~~us~~i~ ~~r~~~~~~ and found that several subfra~e~ts of this piece of mtDNA conferred the ARS phenot~. The nucleotide sequence of the fragment shows: (i) a high A-!-T content (725~); (ii) a perfect consensus ARS sequence (ATTTATA~TA) in the subfragment with the highest ARS activity; (iii) a large number of ARS consensus-reIat~ sequemxs in the other subfragments, even in one lsrcking ARS activity; (iv) several potential hairpin structures. One of them contains the perfect consensus ARS sequence. Nucledc acidNucIeQtide sequence rmitochondrial DNA

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Section: Resultssupporting

confidence: 74%

Structure of a Cephalosporium acremonium mtDNA replicator

Peñalva¹,

Patiño²,

Rubio³

1986

FEBS Letters

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“…A transcript complementary to the yeast mtDNA replication origin ori5 was used as the RNA substrate (de Zamaroczy et al 1984). The amounts of activity seen in the Bio-Rex 70 elution and the final 15-30% gradient peak are underestimated; this is largely the result of saturation of the assay.…”

Section: Purification Of the Yeast Rnase Mrp Activitymentioning

confidence: 99%

“…This 1.1-kb fragment hybridized to a single band on a genomic Fractions assayed include the clarified 150 mM KC1 whole-cell extract, the flowthrough of DEAE-Sephacel, the 250 mM KC1 elution from DEAESephacel, the flowthrough of Bio-Rex 70, the 500 mM KC1 elution from Bio-Rex 70, and the activity peak from a subsequent 15-30% glycerol gradient. The largest species in all lanes is the substrate: a 147-nucleotide RNA transcript from the yeast mitochondrial ori5 region (de Zamaroczy et al 1984) that has been 3'-end-labeled with [32p]pCp. Cleavage products were separated on a 6% polyacrylamide 7 M urea gel (Chang and Clayton 1987a).…”

Section: Cloning Of the Gene For The Yeast Rnase Mrp Rnamentioning

confidence: 99%

Yeast site-specific ribonucleoprotein endoribonuclease MRP contains an RNA component homologous to mammalian RNase MRP RNA and essential for cell viability.

Schmitt¹,

Clayton²

1992

Genes Dev.

105

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RNase MRP is a site-specific ribonucleoprotein endoribonuclease that cleaves RNA sequence complementary to mammalian mitochondrial origins of replication in a manner consistent with a role in primer RNA metabolism. The same activity in the yeast Saccharomyces cerevisiae has recently been identified; it cleaves an RNA substrate complementary to a yeast mitochondrial origin of replication at an exact site of linkage of RNA to DNA. We have purified this yeast enzyme further and detect a single, novel RNA of 340 nucleotides associated with the enzymatic activity. The single-copy nuclear gene for this RNA was sequenced and mapped to the right arm of chromosome XIV. The identity of the clone, as encoding the RNA copurifying with enzymatic activity, was confirmed by a match to the directly determined sequence of the RNA. The gene sequence also identified a 340-nucleotide RNA in total yeast RNA and in purified RNase MRP enzyme preparations. Inspection of the sequence of the yeast RNA revealed homologies to the RNA component of mouse RNase MRP, 49% overall with specific regions of much greater similarity. The flanking regions of the gene showed characteristics of an RNA polymerase Ii transcription unit, including a TATAAA box and a 7/8 match to the yeast cell cycle box UAS. The RNase MRP RNA gene was deleted by insertional replacement and found to be essential for cellular viability, indicating a critical nuclear role for RNase MRP.

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“…Rpo41-primed DNA replication is similar to that observed in mammalian mitochondria. In support of this mechanism, each mtDNA replication origin shares sequence similarity with the heavy-strand replication origin of mammalian mtDNA, including the presence of a transcription promoter and three GC-rich clusters (2,13). RNA synthesized by Rpo41 from mtDNA replication-origin promoters has been detected, and an endoribonuclease that cleaves the synthesized RNA at sites that correspond to regions of transition from RNA to DNA synthesis has been detected (3,49,53).…”

mentioning

confidence: 93%

DNA Recombination-Initiation Plays a Role in the Extremely Biased Inheritance of Yeast [rho⁻] Mitochondrial DNA That Contains the Replication Origin ori5

Ling

Hori

Shibata

2007

Molecular and Cellular Biology

111

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Hypersuppressiveness, as observed inEukaryotic cells gain most of the energy required for cellular functions by oxidative respiration in mitochondria. These organelles contain hundreds to thousands of copies of a mitochondrial DNA (mtDNA) genome that encodes components essential for respiration and protein synthesis. Generally, mitochondrial alleles segregate during vegetative cell growth (vegetative segregation), and all copies of mtDNA within a cell or an individual generally have the same sequence (homoplasmy). In patients with mitochondrial myopathies, the progressive accumulation of mtDNA with large deletions leads to a heteroplasmic state in specific tissues (20; for review, see references 43 and 54). This phenomenon may be caused by the selective replication and/or segregation of mtDNA bearing the deletion; however, genetic analysis of mtDNA processes in mammals has been hampered by the strict maternal inheritance of mitochondria. The yeast Saccharomyces cerevisiae is a suitable model system because of its biparental mtDNA inheritance (14).The extremely biased inheritance of mtDNA with a large deletion is known in S. cerevisiae as "hypersuppressiveness. Two mechanisms for the initiation of mtDNA replication in yeast have been proposed: mitochondrial transcriptional RNA polymerase (Rpo41)-primed initiation and recombination-mediated initiation. Rpo41-primed DNA replication is similar to that observed in mammalian mitochondria. In support of this mechanism, each mtDNA replication origin shares sequence similarity with the heavy-strand replication origin of mammalian mtDNA, including the presence of a transcription promoter and three GC-rich clusters (2, 13). RNA synthesized by Rpo41 from mtDNA replication-origin promoters has been detected, and an endoribonuclease that cleaves the synthesized RNA at sites that correspond to regions of transition from RNA to DNA synthesis has been detected (3,49,53). The intact replication-origin promoter is required for hypersuppressiveness (36). However, this transcription-dependent process is not the sole mechanism for the initiation of mtDNA replication, since both the replication origin and RPO41 are dispensable for the maintenance of [rho Ϫ ] mtDNA (18,53). In addition, it has been reported that the selective inheritance of hypersuppressive [rho Ϫ ] mtDNA relative to nonhypersuppres-* Corresponding author. Mailing address:

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The ori sequences of the mitochondrial genome of a wild-type yeast strain: number, location, orientation and structure

Cited by 86 publications

References 54 publications

Structure of a Cephalosporium acremonium mtDNA replicator

Structure of a Cephalosporium acremonium mtDNA replicator

Yeast site-specific ribonucleoprotein endoribonuclease MRP contains an RNA component homologous to mammalian RNase MRP RNA and essential for cell viability.

DNA Recombination-Initiation Plays a Role in the Extremely Biased Inheritance of Yeast [rho⁻] Mitochondrial DNA That Contains the Replication Origin ori5

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The ori sequences of the mitochondrial genome of a wild-type yeast strain: number, location, orientation and structure

Cited by 86 publications

References 54 publications

Structure of a Cephalosporium acremonium mtDNA replicator

Structure of a Cephalosporium acremonium mtDNA replicator

Yeast site-specific ribonucleoprotein endoribonuclease MRP contains an RNA component homologous to mammalian RNase MRP RNA and essential for cell viability.

DNA Recombination-Initiation Plays a Role in the Extremely Biased Inheritance of Yeast [rho−] Mitochondrial DNA That Contains the Replication Origin ori5

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DNA Recombination-Initiation Plays a Role in the Extremely Biased Inheritance of Yeast [rho⁻] Mitochondrial DNA That Contains the Replication Origin ori5