The Organization of Proteins in the Human Red Blood Cell Membrane

Phospholipid distribution in human En(a—) red cell membranes which lack the major sialoglycoprotein, glycophorin A

“…One attractive possibility would be that the asymmetry is due to specific intrinsic membrane proteins. The major red cell sialoglycoprotein, glycophorin A, is one of the best characterized intrinsic membrane glycoproteins [1,2]. It contains an intramembraneous hydrophobic amino acid sequence which evidently interacts with membrane lipids.…”

“…The human erythrocyte membrane has been extensively studied as a general membrane model [1,2]. It provides the best example of phospholipid assymmetry in biological membranes [3].…”

Partial purification of the D‐glucose transport protein from human erythrocyte membranes by affinity chromatography on wheat germ lectin‐sepharose

“…[ 71). However, the decrease in activity is slow and might also be due to inactivation by cholate [ 11,121. (c) Scans ofthe electrophoretic patterns of samples 4-7 in (a); nomenclature according to [4,16,22]. Molecular masses are indicated (cf.…”

“…Molecular masses are indicated (cf. [4]). The scans of wells 5-7 were done with a 2.5times higher sensitivity than the scan of well 4.…”

“…Upon SDS electrophoresis this component migrates as a diffuse zone in the 4.5region (nomenclature according to [4]) with app. Mr -55 000 [l-3 3.…”