The Organization of Meiotic Chromosomes

Luykx, Peter

doi:10.1016/b978-0-12-147602-1.50013-1

Cited by 9 publications

(2 citation statements)

References 178 publications

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“…This specific meiotic behavior is not due to the lack of development of the specialized sites of microtubular attachment -the kinetochores -, as four active kinetochores have been found in bivalents from different organisms (Luykx, 1974;Solari & Counce, 1977; for reviews of kinetochore structure and function see Nicklas, 1988;and Rieder, 1982).…”

Section: Introductionmentioning

confidence: 99%

An ?axis-like? material in the centromeric region of metaphase-I chromosomes from mouse spermatocytes

Tandler

Solari

1991

Genetica

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This study reports the persistence of axis-like structures in the centromeric region of both homologues during the metaphase-I and anaphase-I stages of meiotic division of mouse spermatocytes. A novel type of silver 'argentaffin' technique (NH4-Ag) is employed. This technique includes the treatment of glutaraldehyde-fixed tissues with dilute ammonium hydroxide followed by a reduction of aldehyde groups with sodium borohydride. Staining is accomplished with ammoniacal silver nitrate in darkness followed by sulfite washing. The lateral elements of synaptonemal complexes and the single chromosomal axes of diplotene spermatocytes show a prominent reactivity with this technique. The pattern of very small grains over condensed chromatin is uniform and gives only a light opacity to the electron beam. The presence of an axis-like structure is seen in every centromeric end of meiotic chromosomes at metaphase I and anaphase I. The chromatin (heterochromatin) that surrounds the centromeric filament and some material distributed in irregular linear arrays along some of the homologues also showed a higher electron opacity than the bulk of deoxyribonucleoprotein. While the former is related to C+ heterochromatin, the latter could represent dispersed material of diplotene axes. It is suggested that the disposal of axial material is differentially delayed at the centromeric regions. The present evidence supports the hypothesis that axial fragments or lateral-element segments persisting at these regions contribute to the cohesiveness of centromeres of sister chromatids during normal disjunction.

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Section: Introductionmentioning

confidence: 99%

An ?axis-like? material in the centromeric region of metaphase-I chromosomes from mouse spermatocytes

Tandler

Solari

1991

Genetica

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“…The pairing consists of two sequential steps; the "chromosome or extrinsic pairing" and the "molecular or intrinsic one" ( Luykx 1974). The present study has revealed that the PP works as the intracellular center of carbohydrate metabolism, and, as the result, makes the cell and nuclear condition suitable for the extrinsic pairing (Hiraoka 1951b), while the MP is the intracellular center of ATP production.…”

Section: Discussionmentioning

confidence: 99%

Cytochemical Characterization of the Synaptic Poles in Equisetum Spore Mother Cells in the Synaptic Stage of Meiosis.

Hiraoka

1997

CYTOLOGIA

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Squash Preparation Studies of Germ Cells in Human Fetal Testes

Sun

Gondos

1984

Journal of Andrology

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Testes from human fetuses 21 to 41 weeks of gestation were investigated using a modified squash technique. At all of the gestational ages examined, 90 to 95% of the germ cells were in interphase. Preleptotene germ cells, which exhibited densely staining condensed clumps of chromosomes, were found in all samples studied and constituted 3 to 9% of the germ cells observed. In appearance they resembled preleptotene figures seen in the fetal ovary. Occasionally, germ cells containing slender chromosomes with a beaded appearance, but without prominent condensation, were encountered. These cells were interpreted as preleptotene germ cells in the early stages of degeneration. Germ cells in other meiotic stages were not observed in this study.

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The Organization of Meiotic Chromosomes

Cited by 9 publications

References 178 publications

An ?axis-like? material in the centromeric region of metaphase-I chromosomes from mouse spermatocytes

An ?axis-like? material in the centromeric region of metaphase-I chromosomes from mouse spermatocytes

Cytochemical Characterization of the Synaptic Poles in Equisetum Spore Mother Cells in the Synaptic Stage of Meiosis.

Squash Preparation Studies of Germ Cells in Human Fetal Testes

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