The Organization and Control of Plant Mitochondrial Metabolism

McDonald, Allison E.; Vanlerberghe, Greg C.

doi:10.1002/9780470988640.ch11

Cited by 12 publications

(10 citation statements)

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“…This observation is in line with the current view of a more relaxed evolution of intron sequences in comparison to exons and suggests strong evolutionary constraints to maintain the functionality of the AOX protein‐coding sequence (Wang et al 2005). AOX plays an important role in alternative respiration in mitochondria and is in plants involved in all types of stress reactions (McDonald and Vanlerberghe 2006, Plaxton and Podestá 2006), including morphogenic responses, such as adaptive growth and development (Campos et al 2009, Fiorani et al 2005, Frederico et al 2009, Ho et al 2007). On the other side, it may be suggested that the higher diversity in AOX intron sequences can point to the role of introns in providing more genetic flexibility to AOX regulation.…”

Section: Discussionmentioning

confidence: 99%

“…Several exceptions from this rule were referred to intron loss and gains (Considine et al 2002, Ito et al 1997, Saisho et al 2001). Studying the occurrence of intron length and sequence polymorphism in members of AOX , an enzyme involved in stress acclimation and adaptation (McDonald and Vanlerberghe 2006, Plaxton and Podestá 2006, Umbach et al 2005), can be relevant for a better understanding of adaptation at evolutionary scale (Frederico et al 2009, McDonald 2008) to study biodiversity and ecology, and for promoting breeding programs on stress tolerance (Arnholdt‐Schmitt et al 2006).…”

Section: Introductionmentioning

confidence: 99%

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Intron polymorphism pattern in AOX1b of wild St John's wort (Hypericum perforatum) allows discrimination between individual plants

Ferreira¹,

Cardoso²,

Macedo³

et al. 2009

Physiologia Plantarum

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The present paper deals with the analysis of natural polymorphism in a selected alternative oxidase (AOX) gene of the medicinal plant, St John's wort. Four partial AOX gene sequences were isolated from the genomic DNA of a wild plant of Hypericum perforatum L. Three genes belong to the subfamily AOX1 (HpAOX1a, b and c) and one to the subfamily AOX2 (HpAOX2). The partial sequence of HpAOX1b showed polymerase chain reaction (PCR) fragment size variation as a result of variable lengths in two introns. PCR performed by Exon Primed Intron Crossing (EPIC)-PCR displayed the same two-band pattern in six plants from a collection. Both fragments showed identical sequences for all exons. However, each of the two introns showed an insertion/deletion (InDel) in identical positions for all plants that counted for the difference in the two fragment sizes. The InDel in intron 1 influenced the predictability of a pre-microRNA site. The almost identical PCR fragment pattern was characterized by a high variability in the sequences. The InDels in both introns were linked to repetitive intron single nucleotide polymorphisms (ISNP)s. The polymorphic pattern obtained by InDels and ISNPs from both fragments together was appropriate to discriminate between all individual plants. We suggest that AOX sequence polymorphism in H. perforatum can be used for studies on gene diversity and biodiversity. Further, we conclude that AOX sequence polymorphism of individual plants should be considered in biological studies on AOX activity to exclude the influence of genetic diversity. The identified polymorphic fragments are available to be explored in future experiments as a potential source for functional marker development related to the characterization of origins/accessions and agronomic traits such as plant growth, development and yield stability.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Intron polymorphism pattern in AOX1b of wild St John's wort (Hypericum perforatum) allows discrimination between individual plants

Ferreira¹,

Cardoso²,

Macedo³

et al. 2009

Physiologia Plantarum

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“…As a consequence, the activity of the pyruvate dehydrogenase complex is highly regulated by a range of allosteric effectors and by covalent modification (Plaxton & Podesta 2006). Mitochondrial pyruvate dehydrogenase and TCA cycle dehydrogenases, such as isocitrate dehydrogenase, demonstrate product inhibition by NADH in vitro (Table 4) (Igamberdiev & Gardestrom 2003; Tovar‐Mendez, Miernyk & Randall 2003; McDonald & Vanlerberghe 2006). Because three reactions of the TCA cycle as well as the intimately associated pyruvate dehydrogenase complex utilize NAD + as a co‐factor, it is not difficult to understand why the cellular ratio of NADH/NAD + or more correctly stated that the mitochondrial NADH/NAD + ratio has a major impact on the flux of carbon through the TCA cycle.…”

Section: The Regulation Of the Tca Cycle Activitymentioning

confidence: 99%

Metabolic control and regulation of the tricarboxylic acid cycle in photosynthetic and heterotrophic plant tissues

Araújo

Nunes‐Nesi

Nikoloski

et al. 2011

Plant Cell & Environment

267

201

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“…In mitochondria, the tricarboxylic acid cycle oxidizes organic acids, converting chemical energy originally stored by photosynthesis back to NAD(P)H. This can be utilized directly for processes requiring reducing power or is oxidized by the mitochondrial electron transport chain (ETC), establishing a proton motive force across the inner mitochondrial membrane that is then used by the mitochondrial ATP synthase (Complex V) to generate ATP. This respiratory ATP then fuels numerous growth and maintenance processes (Fernie et al, 2004;McDonald & Vanlerberghe, 2006;Plaxton & Podesta, 2006;Millar et al, 2011;Tcherkez et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Improved photosynthetic performance during severe drought in Nicotiana tabacum overexpressing a nonenergy conserving respiratory electron sink

2015

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SummaryChloroplasts have means to manage excess reducing power but these mechanisms may become restricted by rates of ATP turnover. Alternative oxidase (AOX) is a mitochondrial terminal oxidase that uncouples the consumption of reducing power from ATP synthesis.Physiological and biochemical analyses were used to compare respiration and photosynthesis of Nicotiana tabacum wild-type (WT) plants with that of transgenic lines overexpressing AOX, under both well-watered and drought stress conditions.With increasing drought severity, AOX overexpression acted to increase respiration in the light (R L ) relative to WT. CO 2 and light response curves indicated that overexpression also improved photosynthetic performance relative to WT, as drought severity increased. This was not due to an effect of AOX amount on leaf water status or the development of the diffusive limitations that occur due to drought. Rather, AOX overexpression dampened photosystem stoichiometry adjustments and losses of key photosynthetic components that occurred in WT.The results indicate that AOX amount influences R L , particularly during severe drought, when cytochrome pathway respiration may become increasingly restricted. This impacts the chloroplast redox state, influencing how the photosynthetic apparatus responds to increasing drought severity. In particular, the development of biochemical limitations to photosynthesis are dampened in plants with increased nonenergy conserving R L .

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The Organization and Control of Plant Mitochondrial Metabolism

Cited by 12 publications

References 162 publications

Intron polymorphism pattern in AOX1b of wild St John's wort (Hypericum perforatum) allows discrimination between individual plants

Intron polymorphism pattern in AOX1b of wild St John's wort (Hypericum perforatum) allows discrimination between individual plants

Metabolic control and regulation of the tricarboxylic acid cycle in photosynthetic and heterotrophic plant tissues

Improved photosynthetic performance during severe drought in Nicotiana tabacum overexpressing a nonenergy conserving respiratory electron sink

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