The Organic Matrix of Bone

Herring, G. M.

doi:10.1016/b978-0-12-119201-3.50012-3

Cited by 74 publications

(33 citation statements)

References 124 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus it has been found that acid mucopolysaccharide synthesis may be related to mechanical stress even when applied to developed cartilage (Akeson et 211. 1958, Hall 1971 and furthermore that such compounds in one way or another may playa role during the process of ossification (Herring 1972 A comparison of the findings of the present study with those of Ponseti et a l., (1968), reveals that the histological structure and the process of ossification of the iliac crest do not seem to be significantly different in dogs from those in human beings.…”

mentioning

confidence: 45%

Autogenous Transplantation of Apophyseal Cartilage to Osteochondral Defects of Joints: An Experimental Study in Dogs

Benum

1974

Acta Orthopaedica Scandinavica

View full text Add to dashboard Cite

mentioning

confidence: 45%

Autogenous Transplantation of Apophyseal Cartilage to Osteochondral Defects of Joints: An Experimental Study in Dogs

Benum

1974

Acta Orthopaedica Scandinavica

View full text Add to dashboard Cite

“…3) presumably represents oxidized lipid material known to be biochemically [1,2] and histochemically [42] present in the calcified bone matrix. Consequently, unosmicated specimens must be used to assess HID-TCH-SP and PA-TCH-SP staining of glycoconjugates in these sites.…”

Section: Discussionmentioning

confidence: 99%

“…Vicinal glycol-containing glycoconjugates, including collagen [1,2] and noncoIlagenous GP [I- 5[, have been biochemically demonstrated in bone matrix. Histochemical studies utilizing periodic acid-Schiff (PAS) staining have demonstrated GPs in osteoblasts, osteoid, and new bone matrix, but not in mature bone [19,20].…”

mentioning

confidence: 99%

Ultrastructural cytochemistry of complex carbohydrates in osteoblasts, osteoid, and bone matrix

et al. 1983

View full text Add to dashboard Cite

Glycosaminoglycans (GAGs) and glycoproteins are essential components for osteogenesis. We have examined rat osteoblasts, osteoid, transitional zone, and fully calcified bone matrix, utilizing Spicer's high-iron diaminethiocarbohydrazide-silver protein (HID-TCH-SP) method for sulfated glycoconjugates and Thiéry's periodate-TCH-SP (PA-TCH-SP) method for vicinal glycol-containing glycoconjugates. HID-TCH-SP stained cytoplasmic granules of osteoblasts. Stain deposits in the extracellular matrix were observed in decreasing amounts in osteoid, the transitional zone, and fully calcified bone matrix. Enzyme digestion with testicular hyaluronidase removed most HID-TCH-SP stain deposits. PA-TCH-SP staining was observed with increasing intensity in rough endoplasmic reticulum, Golgi saccules, and cytoplasmic granules. Collagen fibrils in osteoid were weakly stained with PA-TCH-SP, and their staining appeared even weaker in fully calcified bone matrix. In contrast, collagen fibrils in calcified cartilage stained intensely with the PA-TCH-SP method. Focal circular profiles (0.1-0.5 mumol in diameter), which lacked collagen fibrils but reacted moderately with PA-TCH-SP, were frequently seen in the transitional zone and fully calcified bone matrix, but were only occasionally present in osteoid. The presence of testicular hyaluronidase-resistant GAG and acid phosphatase in these focal areas suggests that they represent sites of GAG degradation. The eventual loss of HID-TCH-SP staining in the bone matrix suggests that removal of sulfated glycoconjugates may be a requisite for expansion of initial calcification sites and/or complete calcification.

show abstract

“…(1) above ( 2 ) Using v, -lop6 miday for fully mineralized bone (Schenk et al, 1982), pm --1.8 g/cm3 for the density of mineral in bone (Herring, 1972), M H A p = lo3 gimol, and D = lop5 cm2is (Robinson and Stokes, 1970), we…”

Section: Diffusion As a Vehicle For Ion Transport In Mineralizing Matmentioning

confidence: 99%

A contribution with review to the description of mineralization of bone and other calcified tissues in vivo

Christoffersen¹,

Landis

1991

Anat. Rec.

116

View full text Add to dashboard Cite

This manuscript considers certain aspects of mineral deposition in bone and other vertebrate calcifying tissues in order to examine physical, chemical, and biological factors important in the mineralization process. The paper in a discussion format principally presents a new data and the formulation of concepts based on such data as well as a summary of background material as necessary review. Mineralization is found to occur at spatially independent sites throughout the organic extracellular tissue matrices. Matrix vesicles and collagen fibrils each may serve as independent nucleation centers for mineral with vesicle mineralization being local and collagen mineralization dominating the tissues as a whole. Collagen fibril organization is suggested to be such that hole zones are aligned in three dimensions, creating extensive channels for mineral accommodation. Nucleation occurs initially in hole zones and crystal growth leads to the development of plate-like mineral particles whose orientation, disposition, and sizes within fibrils are detailed. Effects of diffusion, crystallinity, and critical nucleation and growth events are described with respect to their influence on mineral deposition in bulk and local regions of tissue matrices.

show abstract

The Organic Matrix of Bone

Cited by 74 publications

References 124 publications

Autogenous Transplantation of Apophyseal Cartilage to Osteochondral Defects of Joints: An Experimental Study in Dogs

Autogenous Transplantation of Apophyseal Cartilage to Osteochondral Defects of Joints: An Experimental Study in Dogs

Ultrastructural cytochemistry of complex carbohydrates in osteoblasts, osteoid, and bone matrix

A contribution with review to the description of mineralization of bone and other calcified tissues in vivo

Contact Info

Product

Resources

About