The Organic Acid Profiles in the Feces of Pigs at Brachyspira hyodysenteriae-or B. pilosicoli-Positive Farms

Abstract

ABSTRACT. We observed a significant difference in the organic acid profile of diarrheal feces between pigs infected with and free from pathogenic spirochetes. Diarrhea and loose feces were collected from growing pigs, held at 15 different commercial farms. A total of 106 samples were measured for organic acid concentration by HPLC and were checked for the presence of B. hyodysenteriae and B. pilosicoli by PCR. B. hyodysenteriae was detected in 3 samples collected from one farm. B. pilosicoli was detected in 5 samples collected from another farm. Lower concentrations of iso-butyrate and iso-valerate were likely associated with development of pathogenic spirochete infection. KEY WORDS: organic acid, pathogenic spirochetes, swine.

Probiotic supplementation improves reproductive performance of unvaccinated farmed sows infected with porcine epidemic diarrhea virus

“…Apart from porcine reproductive and respiratory virus (PRRSV) and porcine circovirus type2 (PCV2) (which were positive but not active), a preliminary survey of the farm was negative for the following pathogens in sows and suckling piglets: rotavirus, transmissible gastroenteritis virus (TGEV), Clostridium perfringens , enterotoxigenic Escherichia coli (ETEC), Salmonella sp., Brachyspira hyodysenteriae , Lawsonia intracellularis and classical swine fever virus (CSF). All pathogens except for the CSF virus were tested for by PCR methods as described elsewhere (PRRSV, Inoue, Tsukahara, Sunaba, Itoh, & Ushida, ; PCV2, Sasaki et al., ; rotavirus, Ushida et al., ; TGEV, Kim, Song, & Park, ; C. perfringens , Takahashi, Yoshida, Nakanishi, Tsukahara, & Ushida, ; ETEC and Salmonella spp., Fukushima, Tsunomori, & Seki, ; B. hyodysenteriae , Piao et al., ; L. intracellularis , Suto et al., ). CSF virus has been already eradicated in Japan.…”

An epidemiological survey on pigs showing symptoms of infectious enteric diseases and dyspepsia in Japan

“…Lawsonia intracellularis (Li), Brachyspira hyodysentariae (Bhyo), and B. pilosicoli (Bpilo), were examined by polymerase chain reaction (PCR) according to Suto et al. (2004) or Piao et al. (2007).…”

“…Other bacterial pathogens, i.e. Lawsonia intracellularis (Li), Brachyspira hyodysentariae (Bhyo), and B. pilosicoli (Bpilo), were examined by polymerase chain reaction (PCR) according to Suto et al (2004) or Piao et al (2007). For these PCR detections, bacterial DNA was extracted from fecal samples as described by Godon et al (1997).…”