The order of draw: much ado about nothing?

Indevuyst, Christophe; Schuermans, W.; Bailleul, Els; Meeus, Peter

doi:10.1111/ijlh.12230

Cited by 11 publications

(11 citation statements)

References 14 publications

(24 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additional algorithmic approaches to investigation of abnormal coagulation tests are also available [23,24]. On the other hand, K 2 EDTA contamination up to 13% provides an insignificant bias, which is in support of data recently published by Indevuyst et al [11] and thus confirms that the minimum (if any) cross-contamination of EDTA that may occur during collection of coagulation samples with evacuated tube systems is unlikely to generate any significant bias in results of APTT, PT, and fibrinogen.…”

Section: Discussionsupporting

confidence: 72%

Sodium citrate blood contamination by K₂‐ethylenediaminetetraacetic acid (EDTA): impact on routine coagulation testing

Lima-Oliveira

Salvagno

Danese

et al. 2014

Int J Lab Hematology

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 72%

Sodium citrate blood contamination by K₂‐ethylenediaminetetraacetic acid (EDTA): impact on routine coagulation testing

Lima-Oliveira

Salvagno

Danese

et al. 2014

Int J Lab Hematology

View full text Add to dashboard Cite

show abstract

“…lithium heparin ( 5 ). This evidence has been convincingly contradicted by data reported in recent studies, showing that results of coagulation tests and of those clinical chemistry parameters that are more vulnerable by cross-contamination of ion-chelating additives (i.e., potassium, sodium, calcium, magnesium and phosphate) are not significantly biased by a casual order of draw ( 6 – 10 ).…”

Section: Introductionmentioning

confidence: 81%

Contamination of lithium heparin blood by K2-ethylenediaminetetraacetic acid (EDTA): an experimental evaluation

et al. 2014

View full text Add to dashboard Cite

Introduction:The contamination of serum or lithium heparin blood with ethylenediaminetetraacetic acid (EDTA) salts may affect accuracy of some critical analytes and jeopardize patient safety. The aim of this study was to evaluate the effect of lithium heparin sample contamination with different amounts of K2EDTA.Materials and methods:Fifteen volunteers were enrolled among the laboratory staff. Two lithium heparin tubes and one K2EDTA tube were collected from each subject. The lithium-heparin tubes of each subject were pooled and divided in 5 aliquots. The whole blood of K2EDTA tube was then added in scalar amount to autologous heparinised aliquots, to obtained different degrees of K2EDTA blood volume contamination (0%; 5%; 13%; 29%; 43%). The following clinical chemistry parameters were then measured in centrifuged aliquots: alanine aminotranspherase (ALT), bilirubin (total), calcium, chloride, creatinine, iron, lactate dehydrogenase (LD), lipase, magnesium, phosphate, potassium, sodium.Results:A significant variation starting from 5% K2EDTA contamination was observed for calcium, chloride, iron, LD, magnesium (all decreased) and potassium (increased). The variation of phosphate and sodium (both increased) was significant after 13% and 29% K2EDTA contamination, respectively. The values of ALT, bilirubin, creatinine and lipase remained unchanged up to 43% K2EDTA contamination. When variations were compared with desirable quality specifications, the bias was significant for calcium, chloride, LD, magnesium and potassium (from 5% K2EDTA contamination), sodium, phosphate and iron (from 29% K2EDTA contamination).Conclusions:The concentration of calcium, magnesium, potassium, chloride and LD appears to be dramatically biased by even modest K2EDTA contamination (i.e., 5%). The values of iron, phosphate, and sodium are still reliable up to 29% K2EDTA contamination, whereas ALT, bilirubin, creatinine and lipase appear overall less vulnerable towards K2EDTA contamination.

show abstract

“…We believe that the risk of a bias is low because the tubes were applied to the centrifugation schemes randomly and previous studies did not demonstrate a relevant difference of standard coagulation test results. 25 Fifth, for reasons of practicability not all pairs of samples were analyzed within the 2-week interval recommended by current guidelines but were transferred to a -80°C freezer after several days and analyzed within 2 months. However, all pairs were handled identically, and we do not think that this might have introduced any bias.…”

Section: Strengths and Limitations/implicationsmentioning

confidence: 99%

Rapid Centrifugation in the Routine Hemostasis Laboratory

Wolfensberger

Georgiou²,

Giabbani

et al. 2019

Thromb Haemost

View full text Add to dashboard Cite

Background The use of short and uniform centrifugation schemes contributes significantly to the successful automation of laboratory procedures. It is however unclear if this is applicable to the hemostasis laboratory. Objectives This article assesses the accuracy of measurements obtained with a rapid, high-speed centrifugation scheme in a large set of hemostasis tests, covering the full spectrum of values obtained in clinical practice, and using meaningful statistical measures. Methods Two citrated plasma samples were obtained from consecutive patients of a tertiary hospital with suspected abnormal hemostasis tests and processed with two centrifugation schemes in parallel: 1,500 × g for 10 minutes and 3,137 × g for 7 minutes. The following tests were conducted: prothrombin time (n = 125), international normalized ratio (n = 146), activated partial thromboplastin time (n = 119), thrombin time (n = 105), fibrinogen (n = 125), factor (F)II (n = 69), FV (n = 64), FVII (n = 64), FX (n = 67), FVIII (n = 55), FIX (n = 37), FXI (n = 35), and FXIII (n = 20), D-dimer (n = 34), antithrombin (n = 31), anti-Xa activity (n = 30), von Willebrand antigen (n = 25), and von Willebrand activity (VWF:GPIbM; n = 27). Results A wide range of results were obtained in all tests. Spearman's rank correlation coefficient was at least 0.95 for all tests except FV, FIX, and FXI. The coverage probability π at a given deviation index κ of 15% was above 0.9 for all tests except FV, FVII, FX, FVIII, FIX, FXI, and VWF:GPIbM, suggesting a lack of agreement. Conclusion Our results suggest that high-speed centrifugation is applicable to the majority of routine hemostasis parameters. The coverage probability was more sensitive than Spearman's rank correlation to detect disagreement among centrifugation schemes.

show abstract

The order of draw: much ado about nothing?

Abstract: The order of draw has no significant influence on the PT/INR and APTT when measured on a Sarstedt citrate tube filled without the use of a discard tube or after a heparin, EDTA or serum tube with clot activator.

Cited by 11 publications

References 14 publications

Sodium citrate blood contamination by K₂‐ethylenediaminetetraacetic acid (EDTA): impact on routine coagulation testing

Sodium citrate blood contamination by K₂‐ethylenediaminetetraacetic acid (EDTA): impact on routine coagulation testing

Contamination of lithium heparin blood by K2-ethylenediaminetetraacetic acid (EDTA): an experimental evaluation

Rapid Centrifugation in the Routine Hemostasis Laboratory

Contact Info

Product

Resources

About

The order of draw: much ado about nothing?

Abstract: The order of draw has no significant influence on the PT/INR and APTT when measured on a Sarstedt citrate tube filled without the use of a discard tube or after a heparin, EDTA or serum tube with clot activator.

Cited by 11 publications

References 14 publications

Sodium citrate blood contamination by K2‐ethylenediaminetetraacetic acid (EDTA): impact on routine coagulation testing

Sodium citrate blood contamination by K2‐ethylenediaminetetraacetic acid (EDTA): impact on routine coagulation testing

Contamination of lithium heparin blood by K2-ethylenediaminetetraacetic acid (EDTA): an experimental evaluation

Rapid Centrifugation in the Routine Hemostasis Laboratory

Contact Info

Product

Resources

About

Sodium citrate blood contamination by K₂‐ethylenediaminetetraacetic acid (EDTA): impact on routine coagulation testing

Sodium citrate blood contamination by K₂‐ethylenediaminetetraacetic acid (EDTA): impact on routine coagulation testing