The Order Mycoplasmatales

May, Meghan; Balish, Mitchell F.; Blanchard, Alain

doi:10.1007/978-3-642-30120-9_289

Cited by 19 publications

(18 citation statements)

References 329 publications

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“…Antibiotic therapy before sampling and the time interval between sampling and transportation may have reduced the number of viable mycoplasmas. Generally, PCR shows high sensitivity in mycoplasma detection (11). In agreement with this study, PCR has also led to more positive results in other provinces of Iran (9,14,15).…”

Section: Discussionsupporting

confidence: 87%

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Mixed infection zones may be important in the epidemiology of contagious agalactia

Jafarizadeh

Pourbakhsh

Tadayon

et al. 2016

Journal of Veterinary Research

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Introduction: The current study was designed to detect Mycoplasma agalactiae (Ma), Mycoplasma mycoides subsp. capri (Mmc), Mycoplasma capricolum subsp. capricolum (Mcc) and Mycoplasma putrefaciens (Mp) in sheep and goats with clinical signs consistent with contagious agalactia. Material and Methods: A total of 299 samples were collected from 55 mixed herds in Azarbaijan-e-Sharghi province, Iran. Samples were examined using PCR and culture methods. Results: The findings showed that in 40 herds at least one sample was positive by PCR or culture method. Moreover, out of 274 sheep samples, 101 were proved to be positive using the PCR technique and 76 were found positive using the culture method. Out of 25 goat samples, 10 were found positive using PCR and 9 were positive through the culture method. Less than 20% of isolated mycoplasmas were Ma. Ma was detected from almost all studied regions in the province while Mmc, Mcc, and Mp were detected only in a very limited area that was deemed by the research group the mixed infection zone. Conclusion: In vaccination or eradication projects, it would be more economical to focus on mixed infection zones. Further investigation on mixed infection zones could facilitate better understanding of contagious agalactia epidemiology.

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Section: Discussionsupporting

confidence: 87%

“…PPLO broth cultures were determined positive by colour change and fine cloudiness in the medium. Glycerol was added to the positive PPLO broth cultures and they were then frozen at -70ºC for long-term storage (11,12).…”

Section: Methodsmentioning

confidence: 99%

Mixed infection zones may be important in the epidemiology of contagious agalactia

Jafarizadeh

Pourbakhsh

Tadayon

et al. 2016

Journal of Veterinary Research

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“…It is tempting to speculate that this small genome is the result of an intracellular lifestyle, which was indicated by the microscopic observations of such cells apparently in the cytoplasm of C. tuberculata C2. An intracellular life style is also common for most mycoplasmas sensu lato (May et al ., ). This hypothesis is also compatible with the predicted anaerobic metabolism encoded by this bin.…”

Section: Discussionmentioning

confidence: 99%

The low diverse gastric microbiome of the jellyfish Cotylorhiza tuberculata is dominated by four novel taxa

Viver

Orellana

Hatt

et al. 2017

Environmental Microbiology

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Cotylorhiza tuberculata is an important scyphozoan jellyfish producing population blooms in the Mediterranean probably due to pelagic ecosystem's decay. Its gastric cavity can serve as a simple model of microbial-animal digestive associations, yet poorly characterized. Using state-of-the-art metagenomic population binning and catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH), we show that only four novel clonal phylotypes were consistently associated with multiple jellyfish adults. Two affiliated close to Spiroplasma and Mycoplasma genera, one to chlamydial 'Candidatus Syngnamydia', and one to bacteroidetal Tenacibaculum, and were at least one order of magnitude more abundant than any other bacteria detected. Metabolic modelling predicted an aerobic heterotrophic lifestyle for the chlamydia, which were found intracellularly in Onychodromopsis-like ciliates. The Spiroplasma-like organism was predicted to be an anaerobic fermenter associated to some jellyfish cells, whereas the Tenacibaculum-like as free-living aerobic heterotroph, densely colonizing the mesogleal axis inside the gastric filaments. The association between the jellyfish and its reduced microbiome was close and temporally stable, and possibly related to food digestion and protection from pathogens. Based on the genomic and microscopic data, we propose three candidate taxa: 'Candidatus Syngnamydia medusae', 'Candidatus Medusoplasma mediterranei' and 'Candidatus Tenacibaculum medusae'.

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“…Different and complementary approaches are currently developed using bacteria that have lost much of their genomes through regressive evolution ( 1 – 3 ). Among them, Mollicutes are considered as the best representatives of natural minimal cells with some of the smallest genomes among free-living organisms (0.58 to ∼2.2 Mbp) that can grow in axenic medium ( 4 ). Innovative strategies toward the production of minimalist chassis rapidly emerged from global gene inactivation experiments ( 3 , 5 ) and from comparative genome analyses for a better comprehension of minimal cellular machineries such as the translation apparatus ( 6 ).…”

Section: Introductionmentioning

confidence: 99%

Impact of donor–recipient phylogenetic distance on bacterial genome transplantation

et al. 2016

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Genome transplantation (GT) allows the installation of purified chromosomes into recipient cells, causing the resulting organisms to adopt the genotype and the phenotype conferred by the donor cells. This key process remains a bottleneck in synthetic biology, especially for genome engineering strategies of intractable and economically important microbial species. So far, this process has only been reported using two closely related bacteria, Mycoplasma mycoides subsp. capri (Mmc) and Mycoplasma capricolum subsp. capricolum (Mcap), and the main factors driving the compatibility between a donor genome and a recipient cell are poorly understood. Here, we investigated the impact of the evolutionary distance between donor and recipient species on the efficiency of GT. Using Mcap as the recipient cell, we successfully transplanted the genome of six bacteria belonging to the Spiroplasma phylogenetic group but including species of two distinct genera. Our results demonstrate that GT efficiency is inversely correlated with the phylogenetic distance between donor and recipient bacteria but also suggest that other species-specific barriers to GT exist. This work constitutes an important step toward understanding the cellular factors governing the GT process in order to better define and eventually extend the existing genome compatibility limit.

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The Order Mycoplasmatales

Cited by 19 publications

References 329 publications

Mixed infection zones may be important in the epidemiology of contagious agalactia

Mixed infection zones may be important in the epidemiology of contagious agalactia

The low diverse gastric microbiome of the jellyfish Cotylorhiza tuberculata is dominated by four novel taxa

Impact of donor–recipient phylogenetic distance on bacterial genome transplantation

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