The Optimization and HS-SPME/GC-MS Analysis in Pear Fruit

Abstract

Solid-phase microextraction (SPME) is a rapid sample preparation in volatile analysis. The aromatic volatile compositions of ‘Kuerle’ were analyzed using SPME and gas chromatography-mass spectrometry (GC-MS). The effect of different types of fibers, extraction time, and extraction temperature on the volatile results were compared and discussed. The results were as followed. Results showed that 65μm PDMS/DVB fiber had the best effect of extraction. The optimum factors such as extraction time and temperature were 45 min and 40°C.

A comparative study of volatile flavor components in four types of zaoyu using comprehensive two‐dimensional gas chromatography in combination with time‐of‐flight mass spectrometry

“…This might due to the replacement of compounds with lower molecular weights and lower boiling points F I G U R E 1 Three-dimensional total ion mass spectrometry of volatile compounds of the zaoyu under different extraction conditions. (a) and (b) indicate the samples were extracted for 30 min at 30°C and 50°C, respectively; (c) indicate extracting 10 min at 50°C by those with higher molecular weights and higher boiling points (Feng et al, 2017;Li et al, 2014). For further comparative studies in other temperatures of incubation that Microsoft Excel was used for the comparative analysis of peak table obtained at different temperatures.…”

“…To examine the effect of incubation temperature on the extraction efficiency from zaoyu, the employment of 30°C, 40°C, 50°C, and 60°C was investigated. The effect of temperature on features and response values of volatile compounds could be visualized in by those with higher molecular weights and higher boiling points (Feng et al, 2017;Li et al, 2014). For further comparative studies in other temperatures of incubation that Microsoft Excel was used for the comparative analysis of peak table obtained at different temperatures.…”