The optimal immunization procedure of DNA vaccine pcDNA–TA4–IL-2 of Eimeria tenella and its cross-immunity to Eimeria necatrix and Eimeria acervulina

Song, Xiaoling; Xu, Lixin; Ren, Yan; Huang, Xinmei; Shah, M.A.A.; Li, Xiangrui

doi:10.1016/j.vetpar.2008.10.015

Cited by 64 publications

(53 citation statements)

References 30 publications

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“…However, even though, significant difference in the cecal LS between chickens in the infection group and control group was observed (Figure 1). Similar results have been observed by Song et al [16]. Since it was reported that for chickens orally inoculated with sporulated oocysts of E. tenella, 6-7 days post challenge would be an optimum time for chicken sacrifice and cecal lesion score evaluation [17,18], in this study, chickens were sacrificed on the 7th day post infection and the cecal lesion scores as well as the cytokine level in cecal tonsil were determined.…”

Section: Discussionsupporting

confidence: 84%

Use of a Probiotic to Ameliorate the Growth Rate and the Inflammation of Broiler Chickens Caused by Eimeria tenella Infection

Chen¹,

Chuang²,

Chiang³

et al. 2016

J Anim Res Nutr

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A multistrain formula (MF) consisted of four selected lactic acid bacteria (LAB) strains was evaluated for its function to ameliorate the growth rate and inflammation of broiler chickens caused by Eimeria tenella parasitizing. Thirty one day broiler chickens were divided into 3 groups with 10 chickens in 2 cages per group. These groups were control, infected and MF groups. In control group, chickens were fed with 0.2 ml PBS and not challenged with E. tenella. In MF group, chickens were gavage fed with 0.2 ml of MF (109 CFU/ml) from day 1 to day 23. On day 14, each chicken in both the infected and MF groups was challenged with 5,000 sporulated E. tenella oocysts. Seven days post infection, it was found that the average body weight gain (ABWG) for chickens in the infected and MF groups were 281.51 ± 61.00 and 295.52 ± 62.16 g per chicken, respectively. Chickens in each group were then sacrificed and their cecal tonsils collected. It was observed that feeding MF could reduce the cecal lesion scores (LS) of the Eimeria infected chickens significantly. In addition, for chickens in the MF group, the levels of cecal gene expression of proinflammatory cytokines, such as interleukin (IL)-1β, IL-6, and interferon (IFN)-γ, were found significantly lower, while anti-inflammation cytokine, ie, IL-10, was significantly higher than those of the chickens in the infected group. This study thus suggests that the MF, which previously has been shown to have antagonistic effect against Salmonella infection and the induced inflammation either in live or in heat killed form, may also be useful for the amelioration of the growth rate and the inflammation of broiler chickens caused by E. tenella infection.

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Section: Discussionsupporting

confidence: 84%

Use of a Probiotic to Ameliorate the Growth Rate and the Inflammation of Broiler Chickens Caused by Eimeria tenella Infection

Chen¹,

Chuang²,

Chiang³

et al. 2016

J Anim Res Nutr

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“…A number of factors are influencing efficacy of immunization, for instance, the routes, doses, and timing (Song et al 2009). In this study, the DNA plasmid immunization dose was 100 μg with intramuscular injection two times and an interval of 1 week between injections.…”

Section: Resultsmentioning

confidence: 99%

“…So, there is intense need for alternate approaches, which can circumvent these shortcomings as well as provide sufficient levels of protection. Recent immunological research of recombinant DNA Eimeria vaccines are directed towards this goal (Song et al 2009;Shah et al 2010a;Wu et al 2004). Cytokines are natural modulators of immune response and are being used as vaccine adjuvant to enhance host immune responses induced by DNA vaccines in coccidiosis (Kim et al 1999;Song et al 2000;Xu et al 2006).…”

Section: Introductionmentioning

confidence: 99%

The DNA-induced protective immunity with chicken interferon gamma against poultry coccidiosis

Shah

Song

et al. 2010

Parasitol Res

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The immunogenicity of Eimeria acervulina cSZ-2 and chicken interferon gamma was observed against Eimeria tenella challenge. The chickens were randomly divided into six groups of 24 chicks each. Three groups of chickens were injected with DNA vaccines pVAX1-cSZ2, pVAX1-chIFN-gamma and pVAX1-cSZ2-chIFN-gamma two times (at days 14 and 21) at a dose of 100 microg intramuscularly. Three other groups were kept as control and injected with TE buffer (10 mM Tris-HCl pH 7.6 and 1 mM EDTA). One week following the booster dose, all chickens except the non-infected, non-vaccinated control group were inoculated orally with 5 x 10(4) sporulated oocysts of E. tenella. Seven days post challenge, all chickens were weighted and slaughtered for cecal lesion scoring and oocyst counts. The results demonstrated that cSZ-2 in combination with interferon gamma can protect chickens from coccidiosis by significantly decreasing body weight loss and oocyst excretion reflecting partial protection against E. tenella infection, and further studies are necessary to test for protection against other Eimeria species.

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“…Theoretically, the immune responses by the vector pVAX1 could be ignored. In the previous study, it was reported that experimental animals injected with pVAX1 plasmids did not show any change in protective efficacy against infectious parasites compared with challenged positive controls, indicating that effects by DNA vaccines were not attributed to non-specific factors related to plasmids (Song et al 2009. No difference between pVAX1 control and blank control in mice was found in recent studies (Chen et al 2012;Zhou et al 2012).…”

Section: Discussionmentioning

confidence: 89%

DNA vaccine encoding Haemonchus contortus Actin induces partial protection in Goats

Ren

Wang

et al. 2014

Acta Parasitologica

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Actin is a globular multi-functional protein that forms microfilaments, and participates in many important cellular processes. Previous study found that Haemonchus contortus actin could be recognized by the serum of goats infected with the homology parasite. This indicated that H. contortus actin could be a potential candidate for vaccine. In this study, DNA vaccine encoding H. contortus actin was tested for protection against experimental H. contortus infections in goats. Fifteen goats were allocated into three trial groups. The animals of Actin group were vaccinated with the DNA vaccine on day 0 and 14, and challenged with 5000 infective H. contortus third stage larval (L3) on day 28. An unvaccinated positive control group was challenged with L3 at the same time. An unvaccinated negative control group was not challenged with L3. The results showed that DNA vaccine were transcribed at local injection sites and expressed in vivo post immunizations respectively. For goats in Actin vaccinated group, higher levels of serum IgG, serum IgA and mucosal IgA were produced, the percentages of CD4 + T lymphocytes, CD8 + T lymphocytes and B lymphocytes and the concentrations of TGF-β were increased significantly (P<0.05). Following L3 challenge, the mean eggs per gram feces (EPG) and worm burdens of Actin group were reduced by 34.4% and 33.1%, respectively. This study suggest that recombinant H. contortus Actin DNA vaccine induced partial immune response and has protective potential against goat haemonchosis.

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The optimal immunization procedure of DNA vaccine pcDNA–TA4–IL-2 of Eimeria tenella and its cross-immunity to Eimeria necatrix and Eimeria acervulina

Cited by 64 publications

References 30 publications

Use of a Probiotic to Ameliorate the Growth Rate and the Inflammation of Broiler Chickens Caused by Eimeria tenella Infection

Use of a Probiotic to Ameliorate the Growth Rate and the Inflammation of Broiler Chickens Caused by Eimeria tenella Infection

The DNA-induced protective immunity with chicken interferon gamma against poultry coccidiosis

DNA vaccine encoding Haemonchus contortus Actin induces partial protection in Goats

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