The optical properties of mouse skin in the visible and near infrared spectral regions

Sabino, Caetano P.; Deana, Alessandro Melo; Yoshimura, Tania Mateus; Silva, Daniela de Fátima Teixeira da; França, Cristiane Miranda; Hamblin, Michael R.; Ribeiro, Martha S.

doi:10.1016/j.jphotobiol.2016.03.047

Cited by 102 publications

(86 citation statements)

References 25 publications

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“…In this study, the thickness of the skin and muscle layers was measured by US. The optical attenuation was calculated using the optical properties reported in previous studies [31][32][33]. In addition, the limited bandwidth of the US data acquisition system dictates that the measurements at individual wavelengths are semiquantitative.…”

Section: Discussion and Future Workmentioning

confidence: 99%

Identifying intestinal fibrosis and inflammation by spectroscopic photoacoustic imaging: an animal study in vivo

Zhu

Johnson

Huang

et al. 2018

Biomed. Opt. Express

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Abstract:Crohn's disease (CD) is a chronic autoimmune disease characterized by obstructing intestinal strictures. Conventional imaging modalities can identify the strictures but cannot characterize whether a stricture is predominantly inflammatory or fibrotic. The purpose of this study is to examine the capability of photoacoustic (PA) imaging to characterize intestinal fibrosis and inflammation in vivo. Intestinal strictures in a rat model of CD were imaged with a PA-ultrasound parallel imaging system. Internal and external illuminations were attempted, both with transcutaneous PA signal reception. The PA signal magnitudes acquired at wavelengths targeting individual molecular components and the derived functional information showed significant differences between the inflammatory and fibrotic strictures, consistent with histological inflammation and fibrosis.

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Section: Discussion and Future Workmentioning

confidence: 99%

Identifying intestinal fibrosis and inflammation by spectroscopic photoacoustic imaging: an animal study in vivo

Zhu

Johnson

Huang

et al. 2018

Biomed. Opt. Express

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“…The choice of QC-1 dye with maximum absorption at 737 nm 62 within the optical window from 600 and to 1350 nm 63 with minimal light attenuation may have improved the detection of the signal. Furthermore, the ex vivo in situ nearinfrared fluorescence optical imaging and histopathologic studies confirmed the presence of intracamerally injected CF770/BSA in the right submandibular nodes.…”

Section: Discussionmentioning

confidence: 99%

Active Lymphatic Drainage From the Eye Measured by Noninvasive Photoacoustic Imaging of Near-Infrared Nanoparticles

Yücel

Cardinell

Khattak

et al. 2018

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. To visualize and quantify lymphatic drainage of aqueous humor from the eye to cervical lymph nodes in the dynamic state. METHODS.A near-infrared tracer was injected into the right eye anterior chamber of 10 mice under general anesthesia. Mice were imaged with photoacoustic tomography before and 20 minutes, 2, 4, and 6 hours after injection. Tracer signal intensity was measured in both eyes and right and left neck lymph nodes at every time point and signal intensity slopes were calculated. Slope differences between right and left eyes and right and left nodes were compared using paired t-test. Neck nodes were examined with fluorescence optical imaging and histologically for the presence of tracer. RESULTS.Following right eye intracameral injection of tracer, an exponential decrease in tracer signal was observed from 20 minutes to 6 hours in all mice. Slope differences of the signal intensity between right and left eyes were significant (P < 0.001). Simultaneously, increasing tracer signal was observed in the right neck node from 20 minutes to 6 hours. Slope differences of the signal intensity between right and left neck nodes were significant (P ¼ 0.0051). Ex vivo optical fluorescence imaging and histopathologic examination of neck nodes confirmed tracer presence within submandibular nodes.CONCLUSIONS. Active lymphatic drainage of aqueous from the eye to cervical lymph nodes was measured noninvasively by photoacoustic imaging of near-infrared nanoparticles. This unique in vivo assay may help to uncover novel drugs that target alternative outflow routes to lower IOP in glaucoma and may provide new insights into lymphatic drainage in eye health and disease.

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“…The approach was then repeated using our previously obtained mouse tumor images along with optical parameter values reported in the literature for mouse skin. , respectively [26]. Using these optical parameter values, and our calculated transform coefficients, we calculated a slope of 3.09 to use for the fluorescence depth estimates.…”

Section: In Vivo Tumor Depth Estimationmentioning

confidence: 99%

Noninvasive depth estimation using tissue optical properties and a dual-wavelength fluorescent molecular probe in vivo

Miller¹,

Maji²,

Lam³

et al. 2017

Biomed. Opt. Express

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Translation of fluorescence imaging using molecularly targeted imaging agents for real-time assessment of surgical margins in the operating room requires a fast and reliable method to predict tumor depth from planar optical imaging. Here, we developed a dualwavelength fluorescent molecular probe with distinct visible and near-infrared excitation and emission spectra for depth estimation in mice and a method to predict the optical properties of the imaging medium such that the technique is applicable to a range of medium types. Imaging was conducted at two wavelengths in a simulated blood vessel and an in vivo tumor model. Although the depth estimation method was insensitive to changes in the molecular probe concentration, it was responsive to the optical parameters of the medium. Results of the intra-tumor fluorescent probe injection showed that the average measured tumor sub-surface depths were 1.31 ± 0.442 mm, 1.07 ± 0.187 mm, and 1.42 ± 0.182 mm, and the average estimated sub-surface depths were 0.97 ± 0.308 mm, 1.11 ± 0.428 mm, 1.21 ± 0.492 mm, respectively. Intravenous injection of the molecular probe allowed for selective tumor accumulation, with measured tumor sub-surface depths of 1.28 ± 0.168 mm, and 1.50 ± 0.394 mm, and the estimated depths were 1.46 ± 0.314 mm, and 1.60 ± 0.409 mm, respectively. Expansion of our technique by using material optical properties and mouse skin optical parameters to estimate the sub-surface depth of a tumor demonstrated an agreement between measured and estimated depth within 0.38 mm and 0.63 mm for intra-tumor and intravenous dye injections, respectively. Our results demonstrate the feasibility of dual-wavelength imaging for determining the depth of blood vessels and characterizing the sub-surface depth of tumors in vivo. Leblond, "Macroscopic optical imaging technique for wide-field estimation of fluorescence depth in optically turbid media for application in brain tumor surgical guidance," J.

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The optical properties of mouse skin in the visible and near infrared spectral regions

Cited by 102 publications

References 25 publications

Identifying intestinal fibrosis and inflammation by spectroscopic photoacoustic imaging: an animal study in vivo

Identifying intestinal fibrosis and inflammation by spectroscopic photoacoustic imaging: an animal study in vivo

Active Lymphatic Drainage From the Eye Measured by Noninvasive Photoacoustic Imaging of Near-Infrared Nanoparticles

Noninvasive depth estimation using tissue optical properties and a dual-wavelength fluorescent molecular probe in vivo

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