The Optical And Epr Spectra of Fe2+ and Fe3+ Ions in the Blood of the Chernobyl Clean-Up Worker

EPR and FTIR Spectroscopies Study of Human Blood after Irradiation

“…For example, the protein concentration in the initial sample was of 59% dry weight (DW) and 52% DW after irradiation and lipid concentration of 1% DW and 2.3% DW, correspondingly. On the basis of our previous studies [5] as discriminative for irradiation effects were chosen Amid I and II bands of proteins.…”

“…The study of blood and hemoglobin damage radiation is very important in order to understand the biological effects of ionizing radiation [1][2][3][4][5][6]. Various physical techniques are used to determine structural damage of hemoglobin after irradiation: absorption spectroscopy, IR spectroscopy [2], and Mossbauer and EPR spectroscopy [6,8,9].…”

“…This work is one of our several investigations of the influence of ionizing radiation on human blood [2,4,5,9].…”

“…FTIR spectroscopy has been successfully used to determine structural changes in erythrocytes in blood samples [3] and the molecular level changes in RBC proteins due to laser irradiation [1]. Reference [5] have used FTIR spectroscopy to study changes of blood when exposed to therapeutic doses of radiation from a He-Ne laser and identified changes in the Amide A, Amide I, Amide II, and Amide III absorption regions and also in the absorption bands of methyl, methylene, and phosphate groups. Our previous studies showed that resonance Raman scattering and FTIR spectroscopy indicate the irradiation effects on blood hemoglobin [4].…”

Simultaneous Fe 2+ /Fe 3+ imaging shows Fe 3+ over Fe 2+ enrichment in Alzheimer’s disease mouse brain

“…To achieve sensing of different redox states of iron, various methods have been explored but with limited success either in living cells or in vivo. For example, laboratory techniques, such as inductively coupled plasma mass spectrometry (12,13), electron paramagnetic resonance (14), x-ray fluorescence (15), and magnetic resonance imaging (MRI) (16)(17)(18) have been developed but cannot readily provide spatial or temporal information in vivo because of their restrictive requirements for sample pretreatment or excessive time needed for data collection. It has been shown that "labile" iron pools, which comprise only a small portion of total iron, play critical roles in many cellular processes, including lipid oxidation during ferroptosis and generating free radicals in AD (19)(20)(21), and all of the above methods can measure only the total iron without differentiating labile iron pools.…”