The Opportunity for Microbiopsies for Skin Cancer

Prow, Tarl W.; Lin, Lynlee L.; Soyer, H. Peter

doi:10.2217/fon.13.88

Cited by 4 publications

(1 citation statement)

References 19 publications

(20 reference statements)

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“…First, the naevus for sampling will be identified, measured and photographed (clinical and dermoscopic images) and then will undergo microbiopsy in vivo to obtain 1000–3000 cells. 23 24 The naevus will then undergo a shave excision with 2 mm margins: half of the lesion will be sent for routine pathology examination and the other half will be separated into lesion and perilesional sections and transferred to a 1.5 mL Eppendorf tube containing 500 µL of RNALater solution, 25 26 which will be stored in an 80°C freezer for later genomic DNA and/or total RNA extraction.…”

Section: Methods and Analysismentioning

confidence: 99%

‘Mind your Moles’ study: protocol of a prospective cohort study of melanocytic naevi

et al. 2018

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IntroductionHaving many melanocytic naevi or ‘moles’ on the skin is the strongest predictor of melanoma; thus, much can be learnt from investigating naevi in the general population. We aim to improve the understanding of the epidemiology and biology of naevi by conducting a 3-year prospective study of melanocytic naevi in adults.Methods and analysisThis is a population-based cohort study of melanocytic naevi in 200 adults aged 20–69 years recruited via the Australian electoral roll. At baseline, participants will complete a questionnaire on their sun behaviour and health and undergo a clinical examination. Three-dimensional (3D) total-body photography will be used to record the images of skin lesions. Pigmented naevi will be analysed in terms of number, diameter, colour and border irregularity using automated analysis software (excluding scalp, beneath underwear and soles of feet). All naevi ≥5 mm will be recorded using the integrated dermoscopy photographic system. A saliva sample will be obtained at baseline for genomic DNA analysis of pigmentation, naevus and melanoma-associated genes using the Illumina HumanCoreExome platform. The sun behaviour and health follow-up questionnaire, clinical examination and 3D total-body photography will be repeated every 6 months for 3 years. The first 50 participants will also undergo manual counts of naevi ≥2 mm and ≥5 mm at baseline, 6-month and 12-month follow-ups. Microbiopsy and excision of naevi of research interest is planned to commence at the 18-month time point among those who agree to donate samples for detailed histopathological and molecular assessment.Ethics and disseminationThis study was approved by the Metro South Health Human Research Ethics Committee in April 2016 (approval number: HREC/16/QPAH/125). The findings will be disseminated through peer-reviewed and non-peer-reviewed publications and presentations at conferences.

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Section: Methods and Analysismentioning

confidence: 99%