The oomycete Lagenisma coscinodisci hijacks host alkaloid synthesis during infection of a marine diatom

Vallet, Marine; Baumeister, Tim U. H.; Kaftan, Filip; Grabe, Veit; Buaya, Anthony T.; Thines, Marco; Svatoš, Aleš; Pohnert, Georg

doi:10.1038/s41467-019-12908-w

Cited by 20 publications

(12 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several factors, e.g. chemical defense, genotype adaptation, morphological barriers (frustule thickness, pore size), are known to interfere in host-parasite interaction (Raven and Waite 2004;Pohnert 2005;Pancic et al 2019;Vallet et al 2019;Zhang et al 2017). In crossinfection trials, the spread and rate of infection were noted to be different when two strains of D. perforans s.l.…”

Section: Discussionmentioning

confidence: 99%

Phylogeny and cultivation of the holocarpic oomycete Diatomophthora perforans comb. nov., an endoparasitoid of marine diatoms

et al. 2020

Self Cite

View full text Add to dashboard Cite

Oomycetes infecting diatoms are biotrophic parasitoids and live in both marine and freshwater environments. They are ubiquitous, but the taxonomic affinity of many species remains unclear and the majority of them have not been studied for their molecular phylogeny. Only recently, the phylogenetic and taxonomic placement of some diatom-infecting, early-diverging oomycetes was resolved, including the genera Ectrogella, Miracula, Olpidiopsis, and Pontisma. A group of holocarpic diatom parasitoids with zoospores swarming within the sporangium before release were found to be unrelated to the known genera with diatom-infecting species, and were reclassified to a new genus, Diatomophthora. However, about a dozen species of holocarpic diatom parasitoids with unclear affinity remained unsequenced, which includes a commonly occurring species so far identified as Ectrogella perforans. However, this assignment to Ectrogella is doubtful, as the species was not reported to feature a clear-cut diplanetism, a hallmark of Ectrogella s. str. and the whole class Saprolegniomycetes. It was the aim of the current study to clarify the phylogenetic affinities of the species and if the rather broad host range reported is correct or a reflection of cryptic species. By targeted screening, the parasitoid was rediscovered from Helgoland Roads, North Sea and Oslo Fjord, Southern Norway and investigated for its phylogenetic placement using small ribosomal subunit (18S) sequences. Stages of its life cycle on different marine diatoms were described and its phylogenetic placement in the genus Diatomophthora revealed. A stable host-parasite axenic culture from single spore strains of the parasitoid were established on several strains of Pleurosigma intermedium and Coscinodiscus concinnus. These have been continuously cultivated along with their hosts for more than 2 years, and cultural characteristics are reported. Cross-infection trials revealed the transferability of the strains between hosts under laboratory conditions, despite some genetic distance between the pathogen strains. Thus, we hypothesise that D. perforans might be in the process of active radiation to new host species.

show abstract

Section: Discussionmentioning

confidence: 99%

Phylogeny and cultivation of the holocarpic oomycete Diatomophthora perforans comb. nov., an endoparasitoid of marine diatoms

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Fungal and Amoeba parasites associated with algae cultures are one of the factors related to the regulation of the algae population. These parasites have been shown to have detrimental effects on algal blooms, especially in monocultures, by preventing the algae from proliferating (Carney and Lane, 2014; Vallet et al, 2019). However, there is still a lack of extensive information on their role in algal production.…”

Section: Multi-marker Microbiome Characterization and Effect Of Ald O...mentioning

confidence: 99%

Microalgae growth and diversity in anaerobic digestate compared to synthetic media

Ermiş

Guven-Gulhan²,

Çakır

et al. 2022

Biofuel Res. J.

View full text Add to dashboard Cite

Economizing microalgal cultivation is a considerable milestone targeted by efforts put into microalgal biorefineries. In light of that, the present study was aimed to explore the potential of using anaerobic liquid digestate (ALD) as culture media to grow microalgae and compared it with three different synthetic media (i.e., N8, BBM, and M8) in terms of biomass yield, fatty acid composition, and nutrient utilization/recovery. Moreover, a mixed culture of wild-type microalgae was employed in this study owing to the ability of mixed cultures to survive extreme conditions, eliminating the risk of losing the culture easily, as it mostly happens with pure cultures. The highest nutrient yield coefficients were achieved when the mixed microalgae culture was cultivated in ALD, where the yield coefficient for nitrogen (YN) and yield coefficient for phosphorus (YP) were 10.7 mg biomass mg-1 N and 98 mg biomass mg-1 P, respectively. The highest lipid content (34%) and the highest concentrations of C16:0 (114 mg L-1) and C18:0 (60.9 mg L-1) were also recorded when the mixed microalgae culture was cultivated in ALD. Furthermore, the polyunsaturated fatty acids (PUFA) content also increased significantly in ALD, a beneficial phenomenon as PUFAs in microalgae allow them to adapt more effectively to extreme conditions. Based on the microbial community analysis performed using the multi-marker metabarcoding approach, Diphylleia rotans, Synechocystis PCC-6803, Cyanobium gracile PCC 6307, and Chlorella sorokiniana were identified as the most abundant species in the ALD growth. Overall, based on the findings of the present study, ALD could be used as a promising cultivation medium for microalgae, offering a process integration approach to combine anaerobic digestion and algae cultivation as an effective way to simultaneously treat the high-strength dark-colored ALD and valorize it into profitable byproducts.

show abstract

“…This approach can provide in-depth insights into the physiological responses to (a)biotic stresses, thereby revealing metabolic changes in algal populations (Baumeister et al, 2019). Knowledge about the metabolic processes also allows for the identification of pathogenicity mechanisms (Vallet et al, 2019). Common metabolomics approaches are suitable for tracking the metabolic changes in cultures and complex heterogeneous plankton communities, but they average thousands of cells and neglect cellular individuality.…”

Section: Introductionmentioning

confidence: 99%

Single-cell metabolome profiling for phenotyping parasitic diseases in phytoplankton

Vallet¹,

Kaftan²,

Buaya³

et al. 2023

Front. Anal. Sci.

View full text Add to dashboard Cite

Bloom-forming phytoplankton are key players in aquatic ecosystems, fixing carbon dioxide and forming the base of the marine food web. Diverse stresses, such as nutrient depletion, temperature increase, and pathogen emergence can influence the health and dynamics of algal populations. While population responses to these stressors are well-documented in the aquatic ecosystems, little is known about the individual cellular adaptations. These are however the key to an in-depth physiological understanding of microbiome dynamics in the plankton. Finding solutions to disease control in aquaculture also depends on knowledge of infection dynamics and physiology in algae. Single-cell metabolomics can give insight into infection processes by providing a snapshot of small molecules within a biological system. We used a single-cell metabolome profiling workflow to track metabolic changes of diatoms and dinoflagellates subjected to parasite infection caused by the oomycete Lagenisma coscinodisci and the alveolate Parvilucifera spp. We accurately classified the healthy phenotype of bloom-forming phytoplankton, including the diatoms Coscinodiscus granii and Coscinodiscus radiatus, and the toxic dinoflagellate Alexandrium minutum. We discriminated the infection of the toxic dinoflagellate A. minutum with the alveolate parasitoids Parvilucifera infectans and P. rostrata down to the single-cell resolution. Strain and species-specific responses of the diatom hosts Coscinodiscus spp. Infected with the oomycete pathogen Lagenisma coscinodisci could be recognized. LC-HRMS and fragmentation pattern analysis enabled the structure elucidation of metabolic predictors of infection (guanine, xanthine, DMSP, and pheophorbide). The purine salvage pathway and DMSP lysis could be assigned as regulated processes during host invasion. The findings establish single-cell metabolome profiling with LDI-HRMS coupled with classification analysis as a reliable diagnostic tool to track metabolic changes in algae.

show abstract

The oomycete Lagenisma coscinodisci hijacks host alkaloid synthesis during infection of a marine diatom

Cited by 20 publications

References 41 publications

Phylogeny and cultivation of the holocarpic oomycete Diatomophthora perforans comb. nov., an endoparasitoid of marine diatoms

Phylogeny and cultivation of the holocarpic oomycete Diatomophthora perforans comb. nov., an endoparasitoid of marine diatoms

Microalgae growth and diversity in anaerobic digestate compared to synthetic media

Single-cell metabolome profiling for phenotyping parasitic diseases in phytoplankton

Contact Info

Product

Resources

About