The ontogeny of creatine kinase isozymes

Eppenberger, Hans M.; Eppenberger, Monika E.; Richterich, R.; Aebi, H.

doi:10.1016/0012-1606(64)90002-8

Cited by 253 publications

(107 citation statements)

References 24 publications

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“…Isoenzyme patterns differ strikingly in activity and concentration at various stages of tissue development and differentiation. For example, progression of the isoenzyme pattern from CK-BB to CK-MB to CK-MM has been demonstrated with increasing maturation of chick embryo skeletal muscle (Eppenberger et al, 1964). Since neoplasia is thought to involve alterations in gene expression, the finding of CK-BB and MB in tumor tissue may reflect reversion of neoplastic isoenzyme production to a more primitive form.…”

Section: Discussionmentioning

confidence: 99%

Elevated CK‐MB and CK‐BB in serum and tumor homogenate of a patient with lung cancer

Lee

Bach

Horton

et al. 1985

Clinical Cardiology

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Summary: Elevated serum levels of creatine kinase (CK) were found in a patient with small-(oat) cell carcinoma of the lung. Fractionation of the enzyme showed markedly elevated CK-MB and CK-BB isoenzymes. Clinical and subsequent pathological examination showed no evidence of infarction, inflammation, or tumor involvement of the heart; however, analysis of tumor tissue for CK showed predominance of CK-MB and CK-BB isoenzymes, thus implicating tumor as the source of the circulating levels of CK-MB and CK-BB. Our case is the first to document CK-MB from neoplastic tissue homogenates, and illustrates that markedly elevated circulating levels of CK-MB, or increased levels of CK-MB in combination with CK-BB may point away from a myocardial source, and toward the existence of a malignancy.

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Section: Discussionmentioning

confidence: 99%

Elevated CK‐MB and CK‐BB in serum and tumor homogenate of a patient with lung cancer

Lee

Bach

Horton

et al. 1985

Clinical Cardiology

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“…Eppenberger et al [1] ont montr6 qu'il existe trois types d'isoenzymes de la cr6atine-kinase (ATP cr6atine phosphotransf6rase, EC 2.7.3.2): le type BB du cerveau, le plus charg6 n6gativement fi pH neutre, le type MM propre au muscle stri6 et le type hybride MB pr6sent principalement dans le muscle cardiaque. Ces isoenzymes ont fair l'objet de nombreuses recherches [2].…”

Section: Introductionunclassified

Isolement et proprietes de la creatine‐kinase de muscle lisse de boeuf

Focant¹

1970

FEBS Letters

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Re~u le 17e juillet 1970A method of isolation of the isoenzyme BB of creatine kinase from ox stomach is described taking into account its high instability and the presence of notable amounts of tropomyosin in vertebrate smooth muscle extracts of low ionic strength. The molecular parameters of the enzyme isolated from ox stomach do not differ from those of the creatine kinases MM and BB of vertebrates. The amino acid composition and the peptide maps reveals significant differences from that of the ox brain isoenzyme BB.

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“…In other control experiments we showed that pBR322 DNA bound to solid supports did not retain any RNA translated into M-CK (lanes 2, 7). No M-CK signal was observed when the RNA was absent from the assays (lanes 3,8) or when RNA enriched for globin mRNA was used (lanes 4, 9).…”

Section: Resultsmentioning

confidence: 99%

“…In early stages of differentiation of cultured-myogenic cells as well as in embryonic muscle, BB-CK is accumulated but, as differentiation reaches its terminal phases, isoenzymes containing the muscle-specific CK subunit (M-CK) progressively appear (3)(4)(5) until, in adult muscle, the latter subunit replaces the type B CK subunit (B-CK) previously present (5). In an early transitory phase of terminal differentiation the synthesis of B-CK is enhanced and the synthesis of M-CK is initiated; finally differentiated cells that synthesize predominantly M-CK and only traces of B-CK are produced (6).…”

mentioning

confidence: 99%

“…In the course of differentiation, these proteins replace the cytoplasmic contractile isoproteins, which are expressed in nonmuscle cells and in the nonterminally differentiated myogenic progenitor cells. Hence, isoprotein "switches" occur in differentiating myogenic cells and have been described for some contractile proteins (1, 2) as well as for enzymes such as creatine kinase (CK) (3)(4)(5) that have an important role in energy metabolism in muscle.…”

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confidence: 99%

See 1 more Smart Citation

Molecular cloning and expression during myogenesis of sequences coding for M-creatine kinase.

Rosenberg¹,

Kunz²,

Frischauf³

et al. 1982

Proc. Natl. Acad. Sci. U.S.A.

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Sequences complementary to muscle poly(A)+RNA were cloned in the plasmid pBR322 and the resulting colonies were screened by colony hybridization with labeled cDNA derived from skeletal muscle and smooth muscle (gizzard). The skeletal musclespecific clones were further screened by RNA blotting hybridization for a muscle mRNA having the size expected for a putative type M creatine kinase (M-CK) mRNA. The remaining clones with the expected hybridization properties were finally characterized by hybrid-selected translation, and a cloned sequence was shown to contain DNA hybridizing to mRNA that could be translated into M-CK. This plasmid, pMCKI, was further characterized by restriction mapping. Blot analysis of total cell RNA from differentiating myogenic cell cultures showed accumulation of M-CK mRNA in cultures older than 42 hr but not in young little-differentiated cultures.During the development of myogenic cells, mononucleated cells fuse to form myotubes which become contractile at the time when well-organized myofibrils, the characteristic organelles of cross-striated muscle, appear in the cytoplasm of fully differentiated cells. The myofibrils are composed of a set of myofibrillar contractile proteins that are produced only in terminally differentiated muscle cells. In the course of differentiation, these proteins replace the cytoplasmic contractile isoproteins, which are expressed in nonmuscle cells and in the nonterminally differentiated myogenic progenitor cells. Hence, isoprotein "switches" occur in differentiating myogenic cells and have been described for some contractile proteins (1, 2) as well as for enzymes such as creatine kinase (CK) (3-5) that have an important role in energy metabolism in muscle.In early stages of differentiation of cultured-myogenic cells as well as in embryonic muscle, BB-CK is accumulated but, as differentiation reaches its terminal phases, isoenzymes containing the muscle-specific CK subunit (M-CK) progressively appear (3-5) until, in adult muscle, the latter subunit replaces the type B CK subunit (B-CK) previously present (5). In (Feldbach, Switzerland), and "Gene-Screen" membranes were obtained from New England Nuclear (Dreieich,

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The ontogeny of creatine kinase isozymes

Cited by 253 publications

References 24 publications

Elevated CK‐MB and CK‐BB in serum and tumor homogenate of a patient with lung cancer

Elevated CK‐MB and CK‐BB in serum and tumor homogenate of a patient with lung cancer

Isolement et proprietes de la creatine‐kinase de muscle lisse de boeuf

Molecular cloning and expression during myogenesis of sequences coding for M-creatine kinase.

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