The oncogenic roles of nuclear receptor coactivator 1 in human esophageal carcinoma

Wang, Lu; Li, Weiwei; Li, Kai; Guo, Yi; Liu, Ditian; Yao, Ziting; Lin, Xianjie; Li, Shujun; Jiang, Zuo-Jie; Liu, Qing; Jiang, Yi; Zhang, Beien; Chen, Lei; Zhou, Fuyou; Ren, Hongzheng; Lin, Dong‐Tsamn; Zhang, Dianzheng; Yeung, Sai‐Ching Jim; Zhang, Hao

doi:10.1002/cam4.1786

Cited by 14 publications

(11 citation statements)

References 57 publications

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“…In brief, 3,000 cells were seeded in E-plates, and the plates were locked into the RTCA DP device supplied with humidified air with 5% CO 2 at 37°C. The proliferative ability was monitored by the xCELLigence RTCA Analyzer (Roche Applied Science, Mannheim, Germany) (34, 35).…”

Section: Methodsmentioning

confidence: 99%

MTA3-SOX2 Module Regulates Cancer Stemness and Contributes to Clinical Outcomes of Tongue Carcinoma

Yao

et al. 2019

Front. Oncol.

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Cancer cell plasticity plays critical roles in both tumorigenesis and tumor progression. Metastasis-associated protein 3 (MTA3), a component of the nucleosome remodeling and histone deacetylase (NuRD) complex and multi-effect coregulator, can serve as a tumor suppressor in many cancer types. However, the role of MTA3 in tongue squamous cell cancer (TSCC) remains unclear although it is the most prevalent head and neck cancer and often with poor prognosis. By analyzing both published datasets and clinical specimens, we found that the level of MTA3 was lower in TSCC compared to normal tongue tissues. Data from gene set enrichment analysis (GSEA) also indicated that MTA3 was inversely correlated with cancer stemness. In addition, the levels of MTA3 in both samples from TSCC patients and TSCC cell lines were negatively correlated with SOX2, a key regulator of the plasticity of cancer stem cells (CSCs). We also found that SOX2 played an indispensable role in MTA3-mediated CSC repression. Using the mouse model mimicking human TSCC we demonstrated that the levels of MTA3 and SOX2 decreased and increased, respectively, during the process of tumorigenesis and progression. Finally, we showed that the patients in the MTA3low/SOX2high group had the worst prognosis suggesting that MTA3low/SOX2high can serve as an independent prognostic factor for TSCC patients. Altogether, our data suggest that MTA3 is capable of repressing TSCC CSC properties and tumor growth through downregulating SOX2 and MTA3low/SOX2high might be a potential prognostic factor for TSCC patients.

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Section: Methodsmentioning

confidence: 99%

MTA3-SOX2 Module Regulates Cancer Stemness and Contributes to Clinical Outcomes of Tongue Carcinoma

Yao

et al. 2019

Front. Oncol.

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“…Numerous coregulators of AR have previously been identified, not only in regulating the activation of the AR pathway but also in regulating the proliferation of prostate carcinoma cells. These cofactors often function by directly modulating the transcription factor activation of AR, for example: (i) Modulating the interaction between AR and RNA polymerase II; (ii) modulating the interaction between AR and DNA; and (iii) modulating the widespread unfolding of chromosomes [ 31 – 35 ]. The translocation of AR in cells is of great significance to its well-established activity; however, very few studies have focused on identifying the specific coactivator that promotes the translocation of AR from the cytoplasm to the nucleus.…”

Section: Discussionmentioning

confidence: 99%

Target Protein for Xklp2 Functions as Coactivator of Androgen Receptor and Promotes the Proliferation of Prostate Carcinoma Cells

Sun

Liang

Xiao

et al. 2022

Journal of Oncology

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The activation of the androgen receptor (AR) pathway is crucial in the progression of human prostate cancer. Results of the present study indicated that the target protein xenopus kinesin-like protein (TPX2) enhanced the transcription activation of AR and promoted the proliferation of LNCaP (ligand-dependent prostate carcinoma) cells. The protein-protein interaction between AR and TPX2 was investigated using coimmunoprecipitation assays. Results of the present study further demonstrated that TPX2 enhanced the transcription factor activation of AR and enhanced the expression levels of the downstream gene prostate-specific antigen (PSA). TPX2 did this by promoting the accumulation of AR in the nucleus and also promoting the recruitment of AR to the androgen response element, located in the promoter region of the PSA gene. Overexpression of TPX2 enhanced both the in vitro and in vivo proliferation of LNCaP cells. By revealing a novel role of TPX2 in the AR signaling pathway, the present study indicated that TPX2 may be an activator of AR and thus exhibits potential as a novel target for prostate carcinoma treatment.

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“…However, the ultimate unit of executive function was protein, so when the expression of LRP16 protein was inhibited, the expression of ERα protein was still decreased. The possible reasons for the increase in mRNA level are the participation of ncRNA, the post-transcriptional modi cation of mRNA or the low expression of ERα in ESCC, which leads to a great change in RNA level [41,42] . Similarly, the expression of ERβ decreased accordingly.…”

Section: Discussionmentioning

confidence: 99%

LRP16 Promotes Proliferation and Migration of Esophageal Squamous Cell Carcinoma by Regulating Hippo Signaling Pathway

Liu

Xiong

Wang

et al. 2021

Preprint

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The present study aimed to investigate the expression of LRP16 in the development of ESCC and the relationship between Hippo signaling pathway and LRP16. Immunohistochemistry was used to detect the expression of LRP16 in ESCC tissues. After transfection, the expression of LRP16 was detected by reverse transcription quantitative PCR (RT-qPCR) and western blot techniques. Cell counting kit (CCK-8), clone formation experiment, flow cytometry and wound healing were used to determine the proliferation, apoptosis, cell cycle and migration of ESCC cells. The changes of factors related to Hippo signaling pathway were determined via RT-qPCR and western blot experiments. The results showed that the LRP16 expression in ESCC tissues was higher than that in normal tissues. High expression of LRP16 was related to the depth of invasion, TNM stage and lymph node metastasis of ESCC. Furthermore, the knockdown of LRP16 inhibited proliferation, migration and promoted cell apoptosis and made cells arrested in G2/M phase. It also resulted in decreased expression of Yes-associated protein (YAP), and increased expression of mammalian STE20-like protein kinase (MST1/2), suggesting that LRP16 promoted the development of ESCC through Hippo signaling pathway. The results of this study suggest that LRP16 may be a carcinogenic gene of ESCC and promotes the progression of ESCC through the regulation of Hippo signaling pathway. Our study provides a new idea for the diagnosis and treatment of ESCC in the future.

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The oncogenic roles of nuclear receptor coactivator 1 in human esophageal carcinoma

Cited by 14 publications

References 57 publications

MTA3-SOX2 Module Regulates Cancer Stemness and Contributes to Clinical Outcomes of Tongue Carcinoma

MTA3-SOX2 Module Regulates Cancer Stemness and Contributes to Clinical Outcomes of Tongue Carcinoma

Target Protein for Xklp2 Functions as Coactivator of Androgen Receptor and Promotes the Proliferation of Prostate Carcinoma Cells

LRP16 Promotes Proliferation and Migration of Esophageal Squamous Cell Carcinoma by Regulating Hippo Signaling Pathway

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