The oncogenic impact of RNF2 on cell proliferation, invasion and migration through EMT on mammary carcinoma

Wu, Jin; Wang, Hao; Li, Qing; Guo, Qianying; Tao, Si-Qi; Shen, Yuxian; Wu, Zhengsheng

doi:10.1016/j.prp.2019.152523

Cited by 12 publications

(9 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2a - e ). These data are consistent with recent findings of overexpression and amplification of RNF2 in BRCA 22 , 23 , 31 . The overexpression 31 /amplification of RNF2 , CBX2 , CBX8 and RSF1 were unfavorable prognostic factors for overall survival in invasive BRCA patients ( Extended Data Fig.…”

Section: Resultssupporting

confidence: 93%

“…These data are consistent with recent findings of overexpression and amplification of RNF2 in BRCA 22 , 23 , 31 . The overexpression 31 /amplification of RNF2 , CBX2 , CBX8 and RSF1 were unfavorable prognostic factors for overall survival in invasive BRCA patients ( Extended Data Fig. 2f - i ), in addition to the recently reported negative correlation of RNF2 amplification to the survival in patients with the basal subtype of BRCA 22 .…”

Section: Resultssupporting

confidence: 93%

See 1 more Smart Citation

RNF2 ablation reprograms the tumor-immune microenvironment and stimulates durable NK and CD4+ T-cell-dependent antitumor immunity

et al. 2021

View full text Add to dashboard Cite

Expanding the utility of immune-based cancer treatments is a clinical challenge due to tumor-intrinsic factors that suppress the immune response. Here we report the identification of tumoral Ring Finger Protein 2 (RNF2), the core subunit of the Polycomb Repressor Complex 1 (PRC1), as a negative regulator of antitumor immunity in various human cancers, including breast cancer. In syngeneic murine models of triple negative breast cancer, we found that deleting genes encoding PRC1 subunits Rnf2 or BMI1 proto-oncogene, polycomb ring finger (Bmi1), or the downstream effector of Rnf2, Remodeling and Spacing Factor 1 (Rsf1), was sufficient by itself to induce durable tumor rejection and establish immune memory by enhancing infiltration and activation of NK and CD4 + T-cells, but not CD8 + T-cells, into the tumor and enabled their cooperativity. These findings uncover an epigenetic reprogramming of the tumor-immune microenvironment which fosters durable antitumor immunity and memory.

show abstract

Section: Resultssupporting

confidence: 93%

Section: Resultssupporting

confidence: 93%

RNF2 ablation reprograms the tumor-immune microenvironment and stimulates durable NK and CD4+ T-cell-dependent antitumor immunity

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Studies have shown that the inhibition of MMP2 and/or MMP9 activity or expression decrease the migratory ability of colon cancer cells 35,37,38 . Also, TNF‐α is well‐known for its ability to induce EMT, 39 a process whereby cells lose their epithelial characteristics and gain mesenchymal properties including increased cell migration 40 . Therefore, the suppressed migratory ability of HT‐29 cells could be attributed to the reduction of MMP2, MMP9, and in part TNF‐α protein expression.…”

Section: Resultsmentioning

confidence: 99%

In vitro antimetastatic activity of Momordica balsamina crude acetone extract in HT‐29 human colon cancer cells

Serala

Steenkamp

Mampuru

et al. 2021

Environmental Toxicology

View full text Add to dashboard Cite

Plant‐derived compounds and/or extracts have proven to be beneficial for the treatment of a broad spectrum of cancers with minimal side effects. In this study, we investigated whether a crude acetone extract of Momordica balsamina (MBE) can interfere with the metastatic ability of HT‐29 colorectal cancer (CRC) cells. The phytochemical composition of MBE was determined by ultra‐performance liquid chromatography and cytotoxic effects by the MTT and acridine orange/ethidium bromide staining assays. The effect of MBE on the formation of reactive oxygen species was assessed using the DCFH2‐DA assay. Wound healing assay, transwell cell invasion assay, cell adhesion assay, and the extracellular matrix‐cell adhesion array were used to assess the antimetastatic effects of MBE. The effect of MBE on the expression of TNF‐α, NF‐κB, TIMP‐3, MMP‐2, and MMP‐9 was assessed by western blot analysis. Our results showed that MBE consists of a mixture of compounds without a known anticancer activity in CRC and exhibits cytotoxicity against HT‐29 cells. MBE also suppressed reactive oxygen species formation, cell invasion, cell migration, and cell adhesion. The reduction of cell invasion was associated with the downregulation of TNF‐α, NF‐κB, MMP2, and MMP9 and upregulation of TIMP‐3 proteins. We concluded that MBE inhibits the metastatic ability of HT‐29 CRC cells in vitro.

show abstract

“…demonstrated that RNF1 serves a crucial role in the existence of a redox regulatory mechanism in A. vinelandii, and controls the rate of expression and maturation of nitrogenase by the activity of the Rnf protein complexes. Recent studies reported that RNF2 is highly expressed in malignant tumors and serves a role in promoting cancer (28,29). For example, previous studies demonstrated that RNF2 protein is highly expressed in esophageal, pancreatic, bladder, breast and ovarian cancers, and that RNF2 is manly located in tumor cell nucleus (11,30,31).…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies reported that RNF2 is highly expressed in malignant tumors and serves a role in promoting cancer ( 28 , 29 ). For example, previous studies demonstrated that RNF2 protein is highly expressed in esophageal, pancreatic, bladder, breast and ovarian cancers, and that RNF2 is manly located in tumor cell nucleus ( 11 , 30 , 31 ).…”

Section: Discussionmentioning

confidence: 99%

Clinical significance of ring finger protein 2 high expression in skin squamous cell carcinoma

Bai

Yao²,

Li³

et al. 2020

Oncol Lett

View full text Add to dashboard Cite

Although ring finger protein 2 (RNF2) serves an important role in the occurrence, development and regulation of various types of cancer, RNF2 expression in skin squamous cell carcinoma (SCC) remains unknown. The aim of the present study was to investigate the role of RNF2 expression in SCC and adjacent tissues from patients. The protein and gene expression levels of RNF2 in SCC and adjacent tissues were detected by immunohistochemistry (IHC), western blot analysis and semi-quantitative reverse transcription (RT) PCR. Single factor analysis was used to study the association between RNF2 expression level and the clinicopathological characteristics of patients with SCC. Multifactor Cox survival analysis was used to examine the association between RNF2 expression and the overall survival rate of postoperative patients with SCC. The results from IHC staining demonstrated that the positive expression rate of RNF2 was 84.68% (210/248) and 56.05% (139/248) in SCC and in adjacent tissues, respectively. Furthermore, results from western blot analysis demonstrated that RNF2 protein expression in SCC tissues was significantly higher compared with that in the adjacent tissues (P<0.05). The positive rate of RNF2 mRNA in SCC was 81.05% (201/248), which was significantly higher compared with that in the adjacent tissues 54.44% (135/248; P<0.05). Furthermore, RNF2 protein and gene expression levels were associated with tumor diameter, tumor stage, tumor metastasis and the degree of tumor differentiation in patients with SCC. Patients exhibiting higher RNF2 protein expression in SCC tissues had a significantly shorter disease-specific survival rate compared with patients with low RNF2 expression. In addition, RNF2 protein expression, tumor diameter, tumors site and tumor stage were independent factors affecting the overall survival rate of postoperative patients. High protein and gene expression levels of RNF2 in SCC tissues may be associated with the occurrence and development of SCC and prognosis of patients. The results form this study may serve the development of novel therapeutic options and diagnostic strategies for patients with SCC.

show abstract

The oncogenic impact of RNF2 on cell proliferation, invasion and migration through EMT on mammary carcinoma

Cited by 12 publications

References 31 publications

RNF2 ablation reprograms the tumor-immune microenvironment and stimulates durable NK and CD4+ T-cell-dependent antitumor immunity

RNF2 ablation reprograms the tumor-immune microenvironment and stimulates durable NK and CD4+ T-cell-dependent antitumor immunity

In vitro antimetastatic activity of Momordica balsamina crude acetone extract in HT‐29 human colon cancer cells

Clinical significance of ring finger protein 2 high expression in skin squamous cell carcinoma

Contact Info

Product

Resources

About