The Oncogenic Cysteine-rich LIM domain protein Rbtn2 is essential for erythroid development

Warren, Alan J.; Colledge, William H; Carlton, Mark; Evans, Martin; Smith, Andrew J.H.; Rabbitts, Terence H.

doi:10.1016/0092-8674(94)90571-1

Cited by 565 publications

(429 citation statements)

References 46 publications

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“…Genetic and molecular analyses in mice indicated that these regulators are critical for both primitive (Warren et al, 1994;Shivdasani et al, 1995;Porcher et al, 1996;Robb et al, 1996;Takahashi et al, 1997) and definitive erythropoiesis (Shivdasani et al, 1995;Porcher et al, 1996;Robb et al, 1996;Yamada et al, 1998;Shimizu et al, 2001). We, therefore, asked whether they are also involved in definitive erythropoiesis in chicken yolk sac.…”

Section: Definitive Erythropoiesis In the Yolk Sac Is Accompanied By mentioning

confidence: 99%

Definitive erythropoiesis in chicken yolk sac

Nagai¹,

Sheng²

2008

Developmental Dynamics

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The first wave of erythropoiesis in amniotic animals generates all primitive erythrocytes and takes place exclusively in yolk sac mesoderm. It is less clear, however, to what extend and for how long the yolk sac contributes to the second wave of erythropoiesis which gives rise to definitive erythrocytes for later embryonic and adult use. Here, we examine the initiation, duration, and site of definitive erythrocyte formation in chicken yolk sac. We show that the earliest definitive erythrocytes are generated in yolk sac venous vessels surrounding major arteries at embryonic day (E) 4 -4.5, and that mature definitive erythrocytes enter circulating at E4.5-E5. This takes place at a time when yolk sac vasculature remodels extensively to generate paired arterial/venous vessels. The yolk sac remains the predominant site for definitive erythropoiesis from E5 to E10, and continues to generate definitive erythrocytes at least until E15. Similar to primitive erythropoiesis, definitive erythropoiesis in the yolk sac is accompanied by the expression of transcriptional regulators gata1, scl, and lmo2. Furthermore, our data suggest that one main source of definitive erythropoietic cells is the pre-existing vascular endothelial cells. It remains unclear whether yolk sac derived hematopoietic progenitors that do not undergo erythropoiesis in the yolk sac may take up intraembryonic niches and contribute to erythropoietic stem cell population after hatching.

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Section: Definitive Erythropoiesis In the Yolk Sac Is Accompanied By mentioning

confidence: 99%

Definitive erythropoiesis in chicken yolk sac

Nagai¹,

Sheng²

2008

Developmental Dynamics

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“…insulin) or direct cellular differentiation in cooperation with other nuclear factors (Perez-Alvarado et al, 1994). Other examples include, E47/Pan-1 and Lmx1.1 transcription factors, which have been shown to interact with LMO2, a LIM only protein, and with TAL1, LYL1, and LYL2 to regulate gene activation (Sadler et al, 1992;Warren et al, 1994;Larson et al, 1996;Johnson et al, 1997;Dawid et al, 1998). Similarly, the rat cysteine-rich intestinal protein (CRIP), the human cysteine-rich protein (CRP), and rhombotin-1 and -2 have been implicated as protein crosslinkers in transcription regulation (Dawid et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

A novel IL-10 signalling mechanism regulates TIMP-1 expression in human prostate tumour cells

Wang

Stearns

2003

Br J Cancer

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We have previously reported that interleukin 10 (IL-10) signalling stimulated activation of a specific enhancer element, termed HTE-1, to promote tissue inhibitor of matrix metalloproteinase1 (TIMP-1) expression in human bone metastatic PC-3 subclone (PC-3 ML) cells. Recently, we have identified an IL-10 responsive signal molecule, termed IL-10E1, which binds the HTE-1 element and cloned the gene encoding for the 22 kDa protein. In this paper, we have examined the mechanism of IL-10/IL-10 receptor signalling in two distinct human prostate cell lines, a 'normal' prostate epithelial cell line, termed NPTX-1532 and highly metastatic PC-3 ML tumour cells. Signalling cascade studies revealed that IL-10 stimulated tyrosine phosphorylation of JAK1 and TYK2 receptor kinases and tyrosine phosphorylation of IL-10E1. Phosphorylation, triggered IL-10E1's rapid translocation to the nucleus by 10 -30 min. Deletion analysis combined with transient transfection experiments revealed that the n-terminal domain (B74 a.a.) of the IL-10E1 protein, the nt-nls peptide, was stimulated by IL-10 to translocate to the nucleus and induce TIMP-1 expression. Site-directed mutagenesis further showed that phosphorylation of two tyrosine moieties (Y57 and Y62) of the nt-nls peptide was required for IL-10 activation of signalling and TIMP-1 expression. The data demonstrate, for the first time, that IL-10 receptor signalling of TIMP-1 expression is regulated by tyrosine phosphorylation of a novel gene, IL-10E1, in human prostate cells.

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“…This raises the possibility that Af4 plays a role in both intra and extra-embryonic hematopoiesis (Zion, 1995). The pattern of expression of the putative transcription factor Af4 suggests it plays a role in hematopoiesis and is reminiscent of the hematopoietic transcription factors AML1 (Okuda et al, 1996), GATA2 (Tsai et al, 1994), Ttg2/rbnt2 (Warren et al, 1994) and SCL/tal1 Kehrl, 1995). Af4 expression is down-regulated in some mature B-and T-cells suggesting its down regulation may be required for lymphocyte maturation.…”

Section: Discussionmentioning

confidence: 99%